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Difference between revisions of "Carboxyatractyloside"

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<span  style="color:#8B008B"> '''Caution:''' Chemicals stored in  the fridge  or freezer should be allowed to reach room temperature  before  opening. Very toxic!</span>
<span  style="color:#8B008B"> '''Caution:''' Chemicals stored in  the fridge  or freezer should be allowed to reach room temperature  before  opening. '''Very toxic'''!</span>





Revision as of 07:57, 18 May 2012


high-resolution terminology - matching measurements at high-resolution


Carboxyatractyloside

Description

Carboxyatractyloside is a highly selective and potent inhibitor of the Adenine nucleotide translocator (ANT). Cat stabilizes the nucleoside binding site of ANT on the cytoplasmic side of the inner membrane and blocke the exchange of matrix ATP and cytoplasmic ADP. It causes stabilization of the c conformation of ANT leading to permeability transition pore (PTP) opening, loss of mitochondrial membrane potential, and apoptosis.

Abbreviation: Cat

Reference: MiPNet03.02



MitoPedia topics: Inhibitor 


Application in HRR

Cat: Carboxyatractyloside (Carboxyatractyloside potassium salt from Xanthium sibiricum, C31H44O18S2*Kx); Sigma C 4992, 2 mg, store at -20 °C; FW = 802.99 (free acid basis); Alternative source: Merck 216201-5MG (Calbiochem, Carboxyatractyloside potassium salt from Xanthium sibiricum, C31H43O18S2K3*3H2O), 5 mg, store at -20 °C, FW = 939.1)


Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening. Very toxic!


Preparation of 5 mM stock solution (dissolved in H20):

1) weigh 4.5 mg of Carboxyatractyloside
2) dissolve in 1 ml H2O
3) divide into 0.2 ml aliquots
4) store frozen at -20 °C


Comment: Cat is considered to be much more specific than Atractyloside (Atr), and the effective concentration required is much much lower (µM). With Atr, a very high concentration (mM) is required for full inhibition.


* Talk:Carboxyatractyloside