Description
Carboxyatractyloside is an inhibitor of the Adenine nucleotide translocator (ANT). It is a highly selective inhibitor of cytosolic side-specific mitochondrial ADP/ATP carrier; i.e. adenine nucleotide translocase (ANT); causes stabilization of the c conformation of ANT leading to permeability transition pore (PTP) opening, loss of mitochondrial membrane potential, and apoptosis.
Abbreviation: Cat
Reference: MiPNet03.02
MitoPedia topics: Inhibitor
Application in HRR
Cat: Carboxyatractyloside (Carboxyatractyloside potassium salt from Xanthium sibiricum, C31H44O18S2*Kx); Sigma C 4992, 2 mg, store at -20 °C; FW = 802.99 (free acid basis); Alternative source: Carboxyatractyloside potassium salt from Xanthium sibiricum (C31H43O18S2K3*3H2O) Merck / Calbiochem 216291-5MG, 5 mg, store at -20 °C, FW = 939.1
Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening. Very toxic!
Preparation of 5 mM stock solution (dissolved in H20):
- 1) weigh 4.5 mg of Carboxyatractyloside
- 2) dissolve in 1 ml H2O
- 3) divide into 0.2 ml aliquots
- 4) store frozen at -20 °C
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 30 µl using a 50 µl syringe
- Final conc. in 2 ml O2k-chamber: 0.75 mM
Comment: Cat is considered to be much more specific than Atractyloside (Atr), and the effective concentration required is much much lower (µM). With Atr, a very high concentration (mM) is required for full inhibition.
Cat is available from Sigma (C4992; only 2 mg available), Calbiochem (216201-2MG or 216201-5MG).
* Talk:Carboxyatractyloside