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Difference between revisions of "Carnitine"

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|description='''Carnitine''' is an important factor for the transport of long-chain fatty acids bound to carnitine ([[carnitine acyltransferase]]) into the mitochondrial matrix for subsequent β-oxidation. There are two enantiomers: D- and L-carnitine. Only the L-isomer is physiologically active.
|description='''Carnitine''' is an important factor for the transport of long-chain fatty acids bound to carnitine ([[carnitine acyltransferase]]) into the mitochondrial matrix for subsequent β-oxidation. There are two enantiomers: D- and L-carnitine. Only the L-isomer is physiologically active.
}}
}}
{{MitoPedia topics
__TOC__
|mitopedia topic=Substrate and metabolite
}}
== Application in [[HRR]] ==
== Application in [[HRR]] ==
::::* Car:  '''L-carnitine:''' Sigma Aldrich; C0158; FW=161.2
{{Chemical_description
::::'''Preparation of 250 mM stock solution (dissolved in H<sub>2</sub>O):'''
|abbr=Car
|trivial name=Carnitine
|complete name=L-Carnitine inner salt
|chem formula=C<sub>7</sub>H<sub>15</sub>NO<sub>3</sub>
|molar mass=161.2
|vendor=Sigma-Aldrich
|product number=C0158
|store at=RT
|sensitivity=
|cas=541-15-1
|h statements=
|h info=
}}<!--::::* Car:  '''L-carnitine:''' Sigma Aldrich; C0158; M = 161.2 g·mol<sup>-1</sup>-->
 
 
:::: '''Preparation of 250 mM stock solution''' (dissolved in H<sub>2</sub>O)
 
::::# Weigh 100 mg of Carnitine.
::::# Weigh 100 mg of Carnitine.
::::# Add 2.48 mL H<sub>2</sub>O.
::::# Add 2.48 mL H<sub>2</sub>O.
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::::# Store frozen at -20°C.
::::# Store frozen at -20°C.


:::: '''O2k Manual titration'''
:::» '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]]
::::* Titration volume: 4 µL 250 mM stock using a 10 µL Hamilton syringe (2 mL O2k-Chamber).
 
::::* Titration volume ('''2-mL O2k-chamber'''): 4 µL using a 10 µL Hamilton syringe.
::::* Titration volume ('''0.5-mL O2k-chamber'''): 1 µL using a 10 µL Hamilton syringe
::::* Final concentration: 0.5 mM.
::::* Final concentration: 0.5 mM.




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::::* [[Cahova_2014_Appl_Physiol_Nutr_Metab]]
::::* [[Cahova_2014_Appl_Physiol_Nutr_Metab]]
::::* [[Wessels_2015_Am_J_Physiol]]
::::* [[Wessels_2015_Am_J_Physiol]]
{{MitoPedia topics
|mitopedia topic=Substrate and metabolite
}}
{{Labeling
|additional=MitoPedia:FAT4BRAIN
}}

Latest revision as of 16:39, 18 January 2022


high-resolution terminology - matching measurements at high-resolution


Carnitine

Description

Carnitine is an important factor for the transport of long-chain fatty acids bound to carnitine (carnitine acyltransferase) into the mitochondrial matrix for subsequent β-oxidation. There are two enantiomers: D- and L-carnitine. Only the L-isomer is physiologically active.

Abbreviation: Car

Application in HRR

Car: Carnitine (L-Carnitine inner salt; C7H15NO3), Sigma-Aldrich: C0158, store at RT, CAS: 541-15-1, M = 161.2 g·mol-1


Preparation of 250 mM stock solution (dissolved in H2O)
  1. Weigh 100 mg of Carnitine.
  2. Add 2.48 mL H2O.
  3. Divide into 100 µL portions.
  4. Store frozen at -20°C.
» O2k manual titrations MiPNet09.12 O2k-Titrations
  • Titration volume (2-mL O2k-chamber): 4 µL using a 10 µL Hamilton syringe.
  • Titration volume (0.5-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe
  • Final concentration: 0.5 mM.


Feeding carnitine


MitoPedia topics: Substrate and metabolite 


Labels:






MitoPedia:FAT4BRAIN