Difference between revisions of "Carnitine"
From Bioblast
| (3 intermediate revisions by one other user not shown) | |||
| Line 5: | Line 5: | ||
__TOC__ | __TOC__ | ||
== Application in [[HRR]] == | == Application in [[HRR]] == | ||
::::* Car: '''L-carnitine:''' Sigma Aldrich; C0158; | {{Chemical_description | ||
::::'''Preparation of 250 mM stock solution (dissolved in H<sub>2</sub>O) | |abbr=Car | ||
|trivial name=Carnitine | |||
|complete name=L-Carnitine inner salt | |||
|chem formula=C<sub>7</sub>H<sub>15</sub>NO<sub>3</sub> | |||
|molar mass=161.2 | |||
|vendor=Sigma-Aldrich | |||
|product number=C0158 | |||
|store at=RT | |||
|sensitivity= | |||
|cas=541-15-1 | |||
|h statements= | |||
|h info= | |||
}}<!--::::* Car: '''L-carnitine:''' Sigma Aldrich; C0158; M = 161.2 g·mol<sup>-1</sup>--> | |||
:::: '''Preparation of 250 mM stock solution''' (dissolved in H<sub>2</sub>O) | |||
::::# Weigh 100 mg of Carnitine. | ::::# Weigh 100 mg of Carnitine. | ||
::::# Add 2.48 mL H<sub>2</sub>O. | ::::# Add 2.48 mL H<sub>2</sub>O. | ||
| Line 12: | Line 28: | ||
::::# Store frozen at -20°C. | ::::# Store frozen at -20°C. | ||
::: | :::» '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | ||
::::* Titration volume: 4 µL | |||
::::* Titration volume ('''2-mL O2k-chamber'''): 4 µL using a 10 µL Hamilton syringe. | |||
::::* Titration volume ('''0.5-mL O2k-chamber'''): 1 µL using a 10 µL Hamilton syringe | |||
::::* Final concentration: 0.5 mM. | ::::* Final concentration: 0.5 mM. | ||
| Line 27: | Line 44: | ||
{{MitoPedia topics | {{MitoPedia topics | ||
|mitopedia topic=Substrate and metabolite | |mitopedia topic=Substrate and metabolite | ||
}} | |||
{{Labeling | |||
|additional=MitoPedia:FAT4BRAIN | |||
}} | }} | ||
Latest revision as of 17:39, 18 January 2022
- high-resolution terminology - matching measurements at high-resolution
Carnitine
Description
Carnitine is an important factor for the transport of long-chain fatty acids bound to carnitine (carnitine acyltransferase) into the mitochondrial matrix for subsequent β-oxidation. There are two enantiomers: D- and L-carnitine. Only the L-isomer is physiologically active.
Abbreviation: Car
Application in HRR
- Car: Carnitine (L-Carnitine inner salt; C7H15NO3), Sigma-Aldrich: C0158, store at RT, CAS: 541-15-1, M = 161.2 g·mol-1
- Preparation of 250 mM stock solution (dissolved in H2O)
- Weigh 100 mg of Carnitine.
- Add 2.48 mL H2O.
- Divide into 100 µL portions.
- Store frozen at -20°C.
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 4 µL using a 10 µL Hamilton syringe.
- Titration volume (0.5-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe
- Final concentration: 0.5 mM.
Feeding carnitine
MitoPedia topics:
Substrate and metabolite
Labels:
MitoPedia:FAT4BRAIN
