Carnitine

high-resolution terminology - matching measurements at high-resolution

Carnitine

Description

Carnitine is an important factor for the transport of long-chain fatty acids bound to carnitine (carnitine acyltransferase) into the mitochondrial matrix for subsequent β-oxidation. There are two enantiomers: D- and L-carnitine. Only the L-isomer is physiologically active.

Abbreviation: Car

Application in HRR

• Car: L-carnitine: Sigma Aldrich; C0158; FW=161.2

Preparation of 250 mM stock solution (dissolved in H₂O):

1. Weigh 100 mg of Carnitine.
2. Add 2.48 mL H₂O.
3. Divide into 100 µL portions.
4. Store frozen at -20°C.

O2k Manual titration

• Titration volume: 4 µL 250 mM stock using a 10 µL Hamilton syringe (2 mL O2k-Chamber).
• Final concentration: 0.5 mM.

Feeding carnitine

• Liu_2002_Proc_Natl_Acad_Sci_U_S_A
• La_Guardia_2013_Front_Physiol
• Morand_2014_Eur_J_Nutr
• Cahova_2014_Appl_Physiol_Nutr_Metab
• Wessels_2015_Am_J_Physiol

MitoPedia topics: Substrate and metabolite