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Revision as of 10:48, 22 March 2021 by Spitzer Gudrun (talk | contribs) (added instructions for small-volume-chamber)
Description
Carnitine is an important factor for the transport of long-chain fatty acids bound to carnitine (carnitine acyltransferase) into the mitochondrial matrix for subsequent β-oxidation. There are two enantiomers: D- and L-carnitine. Only the L-isomer is physiologically active.
Abbreviation: Car
Application in HRR
- Car: L-carnitine: Sigma Aldrich; C0158; M = 161.2 g·mol-1
- Preparation of 250 mM stock solution (dissolved in H2O)
- Weigh 100 mg of Carnitine.
- Add 2.48 mL H2O.
- Divide into 100 µL portions.
- Store frozen at -20°C.
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 4 µL using a 10 µL Hamilton syringe.
- Titration volume (0.5-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe
- Final concentration: 0.5 mM.
Feeding carnitine
MitoPedia topics:
Substrate and metabolite