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Difference between revisions of "Chang 2015 Abstract EB2015"

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|title=Different effects of interval and continuous exercise regimens on capacity of mitochondria oxidative phosphorylation in lymphocyte.
|title=Different effects of interval and continuous exercise regimens on capacity of mitochondria oxidative phosphorylation in lymphocyte.
|info=[http://bioblast.at/images/1/15/Chang_2015_Poster_EB2015.pdf Poster]
|info=[http://bioblast.at/images/1/15/Chang_2015_Poster_EB2015.pdf Poster]
|authors=Chang SC, Wang JS
|authors=Chang Shao-Chiang, Wang Jong-Shyan
|year=2015
|year=2015
|event=EB2015
|event=EB2015
|abstract=Mitochondrial biogenesis is a critical metabolic adaptation to aerobic exercise training. What kind of the exercise strategy that enhances mitochondria oxidative phosphorylation in lymphocyte and subsequently improves immune function has not yet been established. This study elucidates how interval and continuous exercise regimens affect capacity of mitochondria oxidative phosphorylation in lymphocytes.ย  ย 
|abstract=Mitochondrial biogenesis is a critical metabolic adaptation to aerobic exercise training. What kind of the exercise strategy that enhances mitochondria oxidative phosphorylation in lymphocyte and subsequently improves immune function has not yet been established. This study elucidates how interval and continuous exercise regimens affect capacity of mitochondria oxidative phosphorylation in lymphocytes.ย  ย 


Twenty-four sedentary males were randomized to perform either aerobic interval training (AIT; 3-minute intervals at 40% and 80%VO<sub>2max</sub>, n=12) or moderate continuous training (MCT; sustained 60%VO<sub>2max</sub>, n=12) for 30 minutes/day, 5 days/week for 6 weeks. According to a novelistically designed Substrate-Uncoupler-Inhibitor Titrations (SUIT) protocol, various modes of mitochondrial respiratory control were analyzed by a high resolution respirometer (OROBOROS Oxygraph-2k). ย 
Twenty-four sedentary males were randomized to perform either aerobic interval training (AIT; 3-minute intervals at 40% and 80%VO<sub>2max</sub>, n=12) or moderate continuous training (MCT; sustained 60%VO<sub>2max</sub>, n=12) for 30 minutes/day, 5 days/week for 6 weeks. According to a novelistically designed Substrate-Uncoupler-Inhibitor Titrations (SUIT) protocol, various modes of mitochondrial respiratory control were analyzed by a high resolution respirometer (Oroboros Oxygraph-2k). ย 


The results demonstrated that both AIT and MCT regimens significantly increased ATP-linked O<sub>2</sub> consumption rate (OCR), elevated reserve capacity of OCR, and lowered non-mitochondrial OCR in intact lymphocytes. Moreover, AIT modestly enhanced pyruvate plus glutamate-mediated OCR, whereas MCT elicited predominant succinate- and palmitoyl carnitine-mediated OCR in permeabilized lymphocytes. ย 
The results demonstrated that both AIT and MCT regimens significantly increased ATP-linked O<sub>2</sub> consumption rate (OCR), elevated reserve capacity of OCR, and lowered non-mitochondrial OCR in intact lymphocytes. Moreover, AIT modestly enhanced pyruvate plus glutamate-mediated OCR, whereas MCT elicited predominant succinate- and palmitoyl carnitine-mediated OCR in permeabilized lymphocytes. ย 
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|area=Respiration, mt-Biogenesis;mt-density, Exercise physiology;nutrition;life style
|area=Respiration, mt-Biogenesis;mt-density, Exercise physiology;nutrition;life style
|organism=Human
|organism=Human
|model cell lines=Macrophage-derived
|tissues=Macrophage-derived
|preparations=Intact cells, Permeabilized cells
|preparations=Permeabilized cells, Intact cells
|pathways=F, N, NS
|pathways=F, N, NS
|instruments=Oxygraph-2k
|instruments=Oxygraph-2k
|additional=TW,
}}
}}
== Affiliations ==
== Affiliations ==
Graduate Inst Rehabilitation Sc, Chang Gung Univ, Tao-Yuan, Taiwan
Graduate Inst Rehabilitation Sc, Chang Gung Univ, Tao-Yuan, Taiwan

Latest revision as of 13:15, 2 March 2020

Different effects of interval and continuous exercise regimens on capacity of mitochondria oxidative phosphorylation in lymphocyte.

Link: Poster

Chang Shao-Chiang, Wang Jong-Shyan (2015)

Event: EB2015

Mitochondrial biogenesis is a critical metabolic adaptation to aerobic exercise training. What kind of the exercise strategy that enhances mitochondria oxidative phosphorylation in lymphocyte and subsequently improves immune function has not yet been established. This study elucidates how interval and continuous exercise regimens affect capacity of mitochondria oxidative phosphorylation in lymphocytes.

Twenty-four sedentary males were randomized to perform either aerobic interval training (AIT; 3-minute intervals at 40% and 80%VO2max, n=12) or moderate continuous training (MCT; sustained 60%VO2max, n=12) for 30 minutes/day, 5 days/week for 6 weeks. According to a novelistically designed Substrate-Uncoupler-Inhibitor Titrations (SUIT) protocol, various modes of mitochondrial respiratory control were analyzed by a high resolution respirometer (Oroboros Oxygraph-2k).

The results demonstrated that both AIT and MCT regimens significantly increased ATP-linked O2 consumption rate (OCR), elevated reserve capacity of OCR, and lowered non-mitochondrial OCR in intact lymphocytes. Moreover, AIT modestly enhanced pyruvate plus glutamate-mediated OCR, whereas MCT elicited predominant succinate- and palmitoyl carnitine-mediated OCR in permeabilized lymphocytes.

Either AIT or MCT effectively enhances capacity of mitochondria oxidative phosphorylation in lymphocytes. Furthermore, AIT has a comprehensive improvement in mitochondria Complex I-linked respiration, while MCT may shift mitochondrial energy transduction into Complex II- and electron transferring flavoprotein-linked respiration.


โ€ข O2k-Network Lab: TW Taoyuan Wang JS


Labels: MiParea: Respiration, mt-Biogenesis;mt-density, Exercise physiology;nutrition;life style 


Organism: Human  Tissue;cell: Macrophage-derived  Preparation: Permeabilized cells, Intact cells 


Pathway: F, N, NS  HRR: Oxygraph-2k 

TW 

Affiliations

Graduate Inst Rehabilitation Sc, Chang Gung Univ, Tao-Yuan, Taiwan