Difference between revisions of "Chavanelle 2015 Fatty Acid Oxidation O2k-Network Discussion Forum"

Latest revision as of 15:57, 7 November 2016

Chavanelle 2015 Fatty Acid Oxidation O2k-Network Discussion Forum

Link: O2k-Network Discussion Forum

Chavanelle V (2015)

Event: Fatty Acid Oxidation O2k-Network Discussion Forum

Fatty oxidation in permeabilised db/db mice muscle fibres, in Mir05 + Cr +37°C. We found that ADP-stimulated respiration in presence of 0,02 mM of palmitoyl-carnitine (OXPHOS) is very close to the non-ADP-stimulated respiration (LEAK state) when working with these conditions. (Values around 14 to 20 (pmol/s/mg w.t.) for LEAK state and 15 to 24 (pmol/s/mg w.t.) for fatty acid triggered-OXPHOS). These data suggest either a very poor ability for this animal model to use palmitoylcarnitine or a very high LEAK state (or both!)

Protocol

2,2 mL mir05+Cr was added in the chambers and oxygen was added to the medium up to 400 – 450 nmol/mL
Chambers were closed
10µM Amplex Red was added to the chambers
2 U HRP was added
10 U SOD was added
20µM Palmitoyl-Carnitine was added
2 mM Malate was added
1 to 3 mg wet permeabilised muscle fibers was added
LEAK state was recorded
Mg+ was added
2,5 mM ADP was added
Pal-C stimulated OXPHOS was recorded

The tissue used was skeletal muscle (Gastrocnemius)

• Keywords: Amplex Red

Labels: Pathology: Diabetes

Organism: Mouse Tissue;cell: Skeletal muscle Preparation: Permeabilized tissue

Coupling state: LEAK, OXPHOS Pathway: F, N

Palmitoylcarnitine

@@ Line 1: / Line 1: @@
 {{Abstract
 |title=Chavanelle 2015 Fatty Acid Oxidation O2k-Network Discussion Forum
 |info=[http://www.bioblast.at/index.php/Talk:Fatty_acid_oxidation O2k-Network Discussion Forum]
 |authors=Chavanelle V
@@ Line 31: / Line 30: @@
 |diseases=Diabetes
 |couplingstates=LEAK, OXPHOS
-|substratestates=CI, FAO
+|pathways=F, N
 |additional=Palmitoylcarnitine
 }}