Difference between revisions of "Chavanelle 2015 Fatty Acid Oxidation O2k-Network Discussion Forum"
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|year=2015 | |year=2015 | ||
|event=Fatty Acid Oxidation O2k-Network Discussion Forum | |event=Fatty Acid Oxidation O2k-Network Discussion Forum | ||
|abstract=Fatty oxidation in permeabilised db/db mice muscle fibres, in Mir05 + Cr +37°C. We found that ADP-stimulated respiration in presence of 0, | |abstract=Fatty oxidation in permeabilised db/db mice muscle fibres, in Mir05 + Cr +37°C. We found that ADP-stimulated respiration in presence of 0,02 mM of palmitoyl-carnitine (OXPHOS) is very close to the non-ADP-stimulated respiration (LEAK state) when working with these conditions. (Values around 14 to 20 (pmol/s/mg w.t.) for LEAK state and 15 to 24 (pmol/s/mg w.t.) for fatty acid triggered-OXPHOS). These data suggest either a very poor ability for this animal model to use palmitoylcarnitine or a very high LEAK state (or both!) | ||
'''Protocol''' | |||
#2,2 mL mir05+Cr was added in the chambers and oxygen was added to the medium up to 400 – 450 nmol/mL | |||
#Chambers were closed | |||
#10µM Amplex Red was added to the chambers | |||
#2 U HRP was added | |||
#10 U SOD was added | |||
#20µM Palmitoyl-Carnitine was added | |||
#2 mM Malate was added | |||
#1 to 3 mg wet permeabilised muscle fibers was added | |||
#LEAK state was recorded | |||
#Mg+ was added | |||
#2,5 mM ADP was added | |||
#Pal-C stimulated OXPHOS was recorded | |||
The tissue used was skeletal muscle (Gastrocnemius) | |||
|keywords=Amplex Red | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
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|tissues=Skeletal muscle | |tissues=Skeletal muscle | ||
|preparations=Permeabilized tissue | |preparations=Permeabilized tissue | ||
|diseases=Diabetes | |||
|couplingstates=LEAK, OXPHOS | |couplingstates=LEAK, OXPHOS | ||
| | |pathways=F, N | ||
|additional=Palmitoylcarnitine | |additional=Palmitoylcarnitine | ||
}} | }} | ||
Latest revision as of 15:57, 7 November 2016
| Chavanelle 2015 Fatty Acid Oxidation O2k-Network Discussion Forum |
Link: O2k-Network Discussion Forum
Chavanelle V (2015)
Event: Fatty Acid Oxidation O2k-Network Discussion Forum
Fatty oxidation in permeabilised db/db mice muscle fibres, in Mir05 + Cr +37°C. We found that ADP-stimulated respiration in presence of 0,02 mM of palmitoyl-carnitine (OXPHOS) is very close to the non-ADP-stimulated respiration (LEAK state) when working with these conditions. (Values around 14 to 20 (pmol/s/mg w.t.) for LEAK state and 15 to 24 (pmol/s/mg w.t.) for fatty acid triggered-OXPHOS). These data suggest either a very poor ability for this animal model to use palmitoylcarnitine or a very high LEAK state (or both!)
Protocol
- 2,2 mL mir05+Cr was added in the chambers and oxygen was added to the medium up to 400 – 450 nmol/mL
- Chambers were closed
- 10µM Amplex Red was added to the chambers
- 2 U HRP was added
- 10 U SOD was added
- 20µM Palmitoyl-Carnitine was added
- 2 mM Malate was added
- 1 to 3 mg wet permeabilised muscle fibers was added
- LEAK state was recorded
- Mg+ was added
- 2,5 mM ADP was added
- Pal-C stimulated OXPHOS was recorded
The tissue used was skeletal muscle (Gastrocnemius)
• Keywords: Amplex Red
Labels: Pathology: Diabetes
Organism: Mouse Tissue;cell: Skeletal muscle Preparation: Permeabilized tissue
Coupling state: LEAK, OXPHOS
Pathway: F, N
Palmitoylcarnitine
