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Description
Condensation of oxaloacetate with acetyl-CoA yields citrate as an entry into the TCA cycle. CS is located in the mt-matrix. CS activity is frequently used as a functional marker of the amount of mitochondria (mitochondrial elementary marker, mtE) for normalization of respiratory flux.
Abbreviation: CS
Reference: MiPNet17.04 CitrateSynthase
Normalization of respiratory flux for CS as a mitochondrial marker
Communicated by Gnaiger Erich last update 2020-04-19
- Respiration of living or permeabilized cells, tissue homogenate or permeabilized tissue, and isolated mitochondria can be normalized for CS activity. Then marker-specific oxygen flux, JO2/CS [pmol∙s-1∙IU-1], is a quantitative measure of respiration that reflects mitochondrial quality, independent of mitochondrial density.
- Several publications in peer-reviewed journals report respiration normalized for CS activity in units which do not make sense, e.g. [pmol∙s-1∙mL-1∙IU-1], such that those numerical results are pointless. Clarification is obtained by strict definitions of normalization of rates (BEC 2020.1) and dimensional analysis, summarized in MiPNet 17.04.
References
Bioblast link | Reference | Year |
---|---|---|
Gnaiger 2020 BEC MitoPathways | Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002 | 2020 |
BEC 2020.1 doi10.26124bec2020-0001.v1 | Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1 | 2020 |
MiPNet17.04 CitrateSynthase | Laboratory protocol: Citrate synthase a mitochondrial marker enzyme. | 2020-04-18 |
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- Quantities for normalization
- » Count in contrast to Number
- » Mitochondrial marker
- » O2k-Protocols: mitochondrial and marker-enzymes
- » Citrate synthase activity
- Quantities for normalization
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MitoPedia topics:
Enzyme