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Difference between revisions of "Closed chamber"

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{{Technical support}}
{{Technical support}}
{{MitoPedia without banner
{{MitoPedia without banner
|abbr=
|abbr=C
|description=The O2k chamber can be used as a [[closed system]] or [[open system]]. Gas bubbles must be avoided.
|description=The O2k-chamber can be used as a [[closed system]] or [[open system]]. Gas bubbles must be avoided.
|info=
|info=[[System]], [[MiPNet22.11 O2k-FluoRespirometer manual]], [[MiPNet24.14 O2k-sV-Module manual]]
}}
}}
== Application in [[HRR]] ==


:::: '''Closed chamber''' means that the chamber and the capillary of the stopper is filled with liquid and no air space or bubble is present in the chamber. For closing the chamber without a bubble an excessive amount of liquid has to be present in the chamber (2.5 mL for 2 mL chamber). The conical shape of the standard stopper facilitates bubble-free closing of the chamber with stirrers switched on - the bubbles will escape through the capillary. If some bubble remains in the chamber, the capillary should be relieved by inserting long needle into the capillary. After closing the chamber the excessive liquid should be aspirated from the top of the stopper.
:::: '''Closed chamber''' means that the experimental chamber and the capillary of the stopper are filled with liquid and no air space or bubble is present in the chamber with the stopper fully inserted into the [[Chamber volume |volume-calibrated position]]. Any titrations are performed between closed states, but not during a closed state. For closing the chamber without a gas bubble, an excessive volume of liquid has to be present in the chamber (minimum 2.1 mL for the 2-mL chamber; 0.54 mL for the 0.5-mL chamber). The stirrer can be switched on. Any foam should be avoided. Before inserting the stopper, the capillary of the stopper must be free of aqueous medium. The conical shape of the standard stopper facilitates bubble-free closing of the chamber with stirrers switched on - the bubbles escape through the central capillary. After closing the chamber the excessive liquid must be aspirated from the receptacle of the stopper. ย 
:::: The procedure of bubble-free closing closingย  a chamber with a multisensor stopper is described in the manual for ion selective electrodes [[MiPNet15.03]].
:::: The procedure of bubble-free closing a chamber with a multisensor stopper is described in the manual for ion selective electrodes [[MiPNet15.03]].


:::: See also: [[Open chamber]]
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== Reconsiderations ==
:::: '''Close C''': Defining an event C as 'closing the chamber' is simple and understandable. But is it understood by a critical mind? A stopper inserted at any position when the O-ring engages with the glass chamber may be considered to close the chamber. Thus the stopper moved slightly up into the stopper-spacer-defined 'open' position technically still maintains the chamber as a closed system with a new volume and a combination of aqueous and gaseous phases. On the other hand, after closing the chamber (marked by C in DatLab), the chamber may be operated as a closed system (the typical mode of operation between titrations in SUIT protocols). But the fact that mass transfer is possible by titrations into the chamber defines the chamber as an open system. Steady-state injections by the TIP-2k are a paradigm of operating the O2k in a continuous open-system mode of operation. Thus the meaning of C may be (or certainly is) ambiguous and thus potentially misleading.
:::: C should stand for "stopper in volume-'''C'''alibrated experimental position".
ย 
::::ยป ''Compare'': [[Open chamber]]
ย 
== Keywords ==
{{Template:Keywords: Chamber volume}}
{{Template:Keywords: Oxygen signal}}
ย 
{{Template:Oroboros QM}} [[Gnaiger Erich]] 2020-09-07

Latest revision as of 16:06, 10 November 2020


                  


O2k-Open Support

Closed chamber



Template NextGen-O2k.jpg


MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



Description

The O2k-chamber can be used as a closed system or open system. Gas bubbles must be avoided.

Abbreviation: C

Reference: System, MiPNet22.11 O2k-FluoRespirometer manual, MiPNet24.14 O2k-sV-Module manual

Application in HRR

Closed chamber means that the experimental chamber and the capillary of the stopper are filled with liquid and no air space or bubble is present in the chamber with the stopper fully inserted into the volume-calibrated position. Any titrations are performed between closed states, but not during a closed state. For closing the chamber without a gas bubble, an excessive volume of liquid has to be present in the chamber (minimum 2.1 mL for the 2-mL chamber; 0.54 mL for the 0.5-mL chamber). The stirrer can be switched on. Any foam should be avoided. Before inserting the stopper, the capillary of the stopper must be free of aqueous medium. The conical shape of the standard stopper facilitates bubble-free closing of the chamber with stirrers switched on - the bubbles escape through the central capillary. After closing the chamber the excessive liquid must be aspirated from the receptacle of the stopper.
The procedure of bubble-free closing a chamber with a multisensor stopper is described in the manual for ion selective electrodes MiPNet15.03.


Reconsiderations

Close C: Defining an event C as 'closing the chamber' is simple and understandable. But is it understood by a critical mind? A stopper inserted at any position when the O-ring engages with the glass chamber may be considered to close the chamber. Thus the stopper moved slightly up into the stopper-spacer-defined 'open' position technically still maintains the chamber as a closed system with a new volume and a combination of aqueous and gaseous phases. On the other hand, after closing the chamber (marked by C in DatLab), the chamber may be operated as a closed system (the typical mode of operation between titrations in SUIT protocols). But the fact that mass transfer is possible by titrations into the chamber defines the chamber as an open system. Steady-state injections by the TIP-2k are a paradigm of operating the O2k in a continuous open-system mode of operation. Thus the meaning of C may be (or certainly is) ambiguous and thus potentially misleading.
C should stand for "stopper in volume-Calibrated experimental position".
ยป Compare: Open chamber

Keywords


Questions.jpg


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ยปBioblast links: Chamber volume - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>



Questions.jpg


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Bioblast links: Oxygen signal - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>


Quality management

Oroboros quality management from the O2k to NextGen-O2k
Gnaiger Erich 2020-09-07