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Difference between revisions of "Coenzyme Q"

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== Application in [[HRR]] ==
:::: '''Q2 : Coenzmye Q2''' (Sigma Aldrich,C8081, MW: 318.41; 2,3-Dimethoxy-5-methyl-6-geranyl-1,4-benzoquinone, Ubiquinone-2, oxidized form)
:::: <span style="color:#8B008B"> '''Caution:''' Light sensitive (store solution in a dark glass vial).</span>.
:::  '''Preparation of 10 mM stock solution''' (dissolved in ethanol) (to run cyclic voltammetry)
::::# Dissolve one commercial vial of Q2 (2 mg) Q2 in 628 µL 99.9% ethanol.
::::# Divide into 50 µL aliquots in dark glass vials.
::::# Store frozen at -20 °C.
::: '''Preparation of 1 mM stock solution''' (to measure the Q redox ratio)
::::# Dilute 50 µL of the 10 mM stock with 450 µL 99.9% ethanol.
::::# Divide into 50 µL aliquots in dark glass vials.
::::# Store frozen at -20 °C.
::: '''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
:::: '''Q redox ratio'''
::::* Titration volume: 2 µL of the 1 mM stock solution using a 10 µL syringe (2 mL O2k-Chamber).
::::* Final concentration: 1 µM
:::: '''Cyclic voltammetry'''
::::* Titration volume: 6 µL of the 10 mM stock solution using a 10 µL syringe (2 mL O2k-Chamber).
::::* Final concentration: 30 µM
{{MitoPedia concepts}}
{{MitoPedia concepts}}
{{MitoPedia methods}}
{{MitoPedia methods}}

Revision as of 10:19, 3 September 2019


high-resolution terminology - matching measurements at high-resolution


Coenzyme Q

Description

Coenzyme Q, redox system (oxidized ubiquinone, partially reduced semiquinone, fully reduced ubiquinol) of the ET-pathway.

More details » Q-junction.

Abbreviation: Q, CoQ

Reference: Gnaiger 2019 MitoPathways


Application in HRR

Q2 : Coenzmye Q2 (Sigma Aldrich,C8081, MW: 318.41; 2,3-Dimethoxy-5-methyl-6-geranyl-1,4-benzoquinone, Ubiquinone-2, oxidized form)
Caution: Light sensitive (store solution in a dark glass vial)..
Preparation of 10 mM stock solution (dissolved in ethanol) (to run cyclic voltammetry)
  1. Dissolve one commercial vial of Q2 (2 mg) Q2 in 628 µL 99.9% ethanol.
  2. Divide into 50 µL aliquots in dark glass vials.
  3. Store frozen at -20 °C.


Preparation of 1 mM stock solution (to measure the Q redox ratio)
  1. Dilute 50 µL of the 10 mM stock with 450 µL 99.9% ethanol.
  2. Divide into 50 µL aliquots in dark glass vials.
  3. Store frozen at -20 °C.


O2k manual titrations MiPNet09.12 O2k-Titrations
Q redox ratio
  • Titration volume: 2 µL of the 1 mM stock solution using a 10 µL syringe (2 mL O2k-Chamber).
  • Final concentration: 1 µM


Cyclic voltammetry
  • Titration volume: 6 µL of the 10 mM stock solution using a 10 µL syringe (2 mL O2k-Chamber).
  • Final concentration: 30 µM








MitoPedia topics: Enzyme, Substrate and metabolite