Difference between revisions of "Coenzyme Q"
From Bioblast
Line 10: | Line 10: | ||
== Application in [[HRR]] == | == Application in [[HRR]] == | ||
:::: '''Q2 : | :::: '''Q2 : Coenzyme Q2''' (Sigma Aldrich, C8081, MW: 318.41; 2,3-Dimethoxy-5-methyl-6-geranyl-1,4-benzoquinone, Ubiquinone-2, oxidized form) | ||
:::: <span style="color:#8B008B"> '''Caution:''' Light sensitive (store solution in a dark glass vial).</span> | :::: <span style="color:#8B008B"> '''Caution:''' Light sensitive (store solution in a dark glass vial).</span> |
Revision as of 10:20, 3 September 2019
Description
Coenzyme Q, redox system (oxidized ubiquinone, partially reduced semiquinone, fully reduced ubiquinol) of the ET-pathway.
More details » Q-junction.
Abbreviation: Q, CoQ
Reference: Gnaiger 2019 MitoPathways
Application in HRR
- Q2 : Coenzyme Q2 (Sigma Aldrich, C8081, MW: 318.41; 2,3-Dimethoxy-5-methyl-6-geranyl-1,4-benzoquinone, Ubiquinone-2, oxidized form)
- Caution: Light sensitive (store solution in a dark glass vial).
- Preparation of 10 mM stock solution (dissolved in ethanol) (to run cyclic voltammetry)
- Dissolve one commercial vial of Q2 (2 mg) Q2 in 628 µL 99.9% ethanol.
- Divide into 50 µL aliquots in dark glass vials.
- Store frozen at -20 °C.
- Preparation of 1 mM stock solution (to measure the Q redox ratio)
- Dilute 50 µL of the 10 mM stock with 450 µL 99.9% ethanol.
- Divide into 50 µL aliquots in dark glass vials.
- Store frozen at -20 °C.
- O2k manual titrations MiPNet09.12 O2k-Titrations
- Q redox ratio
- Titration volume: 2 µL of the 1 mM stock solution using a 10 µL syringe (2 mL O2k-Chamber).
- Final concentration: 1 µM
- Cyclic voltammetry
- Titration volume: 6 µL of the 10 mM stock solution using a 10 µL syringe (2 mL O2k-Chamber).
- Final concentration: 30 µM
MitoPedia topics: Enzyme, Substrate and metabolite