Cottingham 1983 Biochim Biophys Acta

Cottingham IR, Moore AL (1983) Ubiquinone pool behaviour in plant mitochondria. Biochim Biophys Acta 724:191-200.

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Cottingham IR, Moore AL (1983) Biochim Biophys Acta

Abstract: A kinetic analysis of oxygen uptake was carried out in order to investigate the role of ubiquinone pool behaviour in plant mitochondria. The interaction of the external NADH dehydrogenase with either the cytochrome system or the cyanide-insensitive oxidase was examined under various conditions. The involvement of a ubiquinone pool can be deduced from the shape of the titration curve as the appropriate oxidase system is inhibited, by antimycin A for the cytochrome system and salicylhydroxamic acid for the cyanide-insensitive oxidase, at different activities of the NADH dehydrogenase. In the absence of a specific inhibitor, the turnover of the external NADH dehydrogenase was adjusted using a novel NADH-generating system involving the recycling of a low concentration of NAD+ by added glucose 6-phosphate dehydrogenase in the presence of substrate. The results show that ubiquinone pool behaviour is observed between the external NADH dehydrogenase and either the cytochrome b-c1 complex or the cyanide-insensitive oxidase. However, there is a substantial departure from pool behaviour during the simultaneous operation of both oxidases.

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