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Coupling-control efficiency

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{{MitoPedia |abbr=CCC |description=Coupling control capacities, CCC, are flux control capacities, FCC, at a constant mitochondrial substrate state. In mitochondrial preparations, there are three well-defined coupling states of respiration, L, P, E (LEAK, OXPHOS, ETS). In intact cells, state P cannot be induced, but a ROUTINE state of respiration, R, can be measured.

1. If the metabolic control variable, X, is an uncoupler, the reference state Z is E. Then two background states, Y, of coupling control are possible: The uncoupler may act on the L or P state in mt-preparations, and on the L or R state in intact cells. The corresponding coupling control capacities are:

  • E-L coupling control capacity, Ξ”jE-L = (E-L)/E = 1-L/E (mt-preparations and intact cells)
  • E-P coupling control capacity, Ξ”jE-P = (E-P)/E = 1-P/E (mt-preparations)
  • E-P coupling control capacity, Ξ”jE-P = (E-P)/E = 1-P/E (intact cells)

2. If the metablic control variable is stimulation by ADP, D, or release of an inhibitor of DT-phosphorylation (e.g. -Omy), the reference state Z is P in mt-preparations at saturating [[ADP], or R in intact cells at physiologically controlled steady states of ADP and ATP-turnover.

or P, and the such that the lower and upper limits of CCR are defined as 0.0 and 1.0. Then there are two mitochondrial CCR, L/E and P/E, and two CCR for intact cells, L/E and R/E. |info=Gnaiger 2009 Int J Biochem Cell Biol, Pesta 2012 Methods Mol Biol }}

MitoPedia methods: Respirometry 


MitoPedia topics: "Respiratory control ratio" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property. Respiratory control ratio"Respiratory control ratio" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property. 

See also