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Difference between revisions of "DTPA"

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{{MitoPedia
{{MitoPedia
|abbr=DTPA
|abbr=DTPA
|description='''DTPA''' (Diethylentriamin-N,N,N',N,N-penta acetic acid, "Triplex V") is a chelator of transition metals, not cell membrane permeable.  
|description='''DTPA''' (Diethylentriamin-N,N,N',N,N-pentaacetic acid, pentetic acid) is a chelator of transition metals, not cell membrane permeable. general chelates multivalent ions stronger than EDTA; pKa1 = 2.14, pKa2 = 2.38, pKa3 = 4.26 pKa4 = 8.60, pKa5 = 10.53
}}
}}


== Description ==
== Description ==


::::* CAS: 67-43-6
::::* '''CAS:''' 67-43-6
::::* Source: EHERE13095000 VWR 0.25 g
::::* '''Source:''' EHRENSTORFER GMBH (EHERE13095000) or Sigma Aldrich (D-1133).
::::* M: 393,35 g/mol
::::* '''FW:''' 393.35
::::* Stability constants not included in NIST version of Martell 1989, constants from [http://www.dojindo.com/Images/Product%20Photo/Chelate_Table_of_Stability_Constants.pdf]: Ca2+: 10.74 (EDTA 10.96), Mg2+: 9.3 (EDTA: 8.69) Fe II 16.5 (EDTA 14.3), Fe III 28.6 (EDTA 25.1) Mn II 15.6 (EDTA 14.04), ZN 18.75 (EDTA 16.5), general chelates multivalent ions stronger than EDTA; pKa1 = 2.14, pKa2 = 2.38, pKa3 = 4.26 pKa4 = 8.60, pKa5 = 10.53
::::* '''Solubility in H<sub>2</sub>O:''' ~ 4,8 g/l at 25 °C = 12 mM
::::* Solubility in H2O: ca 4,8 g·l−1 bei 25 °C = 12 mM
::::* '''Stability constant:''' see: [http://www.dojindo.com/Images/Product%20Photo/Chelate_Table_of_Stability_Constants.pdf]:  
:::::{| class="wikitable"
|-
!Metal !!  DTPA !! EDTA
|-
|Ca(II)|| 10.74 ||10.96
|-
|Mg(II)|| 9.3 ||8.69
|-
|Fe(II)|| 16.5 ||14.3
|-
|Fe(III) || 28.6 ||25.1
|-
|Mn(II) || 15.6 ||14.04
|-
|Zn (II) || 18.75 || 16.5
|-
|}
 


== DTPA in O2k-FluoRespirometry ==
== DTPA in O2k-FluoRespirometry ==
::::* Final concentration in O2k-chamber: 15 µM
::::* Final concentration in O2k-chamber: 15 µM
::::* Stock solution:
::::* Stock solution:

Revision as of 13:03, 22 August 2017


high-resolution terminology - matching measurements at high-resolution


DTPA

Description

DTPA (Diethylentriamin-N,N,N',N,N-pentaacetic acid, pentetic acid) is a chelator of transition metals, not cell membrane permeable. general chelates multivalent ions stronger than EDTA; pKa1 = 2.14, pKa2 = 2.38, pKa3 = 4.26 pKa4 = 8.60, pKa5 = 10.53

Abbreviation: DTPA


Description

  • CAS: 67-43-6
  • Source: EHRENSTORFER GMBH (EHERE13095000) or Sigma Aldrich (D-1133).
  • FW: 393.35
  • Solubility in H2O: ~ 4,8 g/l at 25 °C = 12 mM
  • Stability constant: see: [1]:
Metal DTPA EDTA
Ca(II) 10.74 10.96
Mg(II) 9.3 8.69
Fe(II) 16.5 14.3
Fe(III) 28.6 25.1
Mn(II) 15.6 14.04
Zn (II) 18.75 16.5


DTPA in O2k-FluoRespirometry

  • Final concentration in O2k-chamber: 15 µM
  • Stock solution: