Difference between revisions of "DTPA"
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{{MitoPedia | {{MitoPedia | ||
|abbr=DTPA | |abbr=DTPA | ||
|description='''DTPA''' (Diethylentriamin-N,N,N',N,N- | |description='''DTPA''' (Diethylentriamin-N,N,N',N,N-pentaacetic acid, pentetic acid) is a chelator of transition metals, not cell membrane permeable. general chelates multivalent ions stronger than EDTA; pKa1 = 2.14, pKa2 = 2.38, pKa3 = 4.26 pKa4 = 8.60, pKa5 = 10.53 | ||
}} | }} | ||
== Description == | == Description == | ||
::::* CAS: 67-43-6 | ::::* '''CAS:''' 67-43-6 | ||
::::* Source: EHERE13095000 | ::::* '''Source:''' EHRENSTORFER GMBH (EHERE13095000) or Sigma Aldrich (D-1133). | ||
::::* | ::::* '''FW:''' 393.35 | ||
::::* Stability | ::::* '''Solubility in H<sub>2</sub>O:''' ~ 4,8 g/l at 25 °C = 12 mM | ||
::::* '''Stability constant:''' see: [http://www.dojindo.com/Images/Product%20Photo/Chelate_Table_of_Stability_Constants.pdf]: | |||
:::::{| class="wikitable" | |||
|- | |||
!Metal !! DTPA !! EDTA | |||
|- | |||
|Ca(II)|| 10.74 ||10.96 | |||
|- | |||
|Mg(II)|| 9.3 ||8.69 | |||
|- | |||
|Fe(II)|| 16.5 ||14.3 | |||
|- | |||
|Fe(III) || 28.6 ||25.1 | |||
|- | |||
|Mn(II) || 15.6 ||14.04 | |||
|- | |||
|Zn (II) || 18.75 || 16.5 | |||
|- | |||
|} | |||
== DTPA in O2k-FluoRespirometry == | == DTPA in O2k-FluoRespirometry == | ||
::::* Final concentration in O2k-chamber: 15 µM | ::::* Final concentration in O2k-chamber: 15 µM | ||
::::* Stock solution: | ::::* Stock solution: |
Revision as of 13:03, 22 August 2017
Description
DTPA (Diethylentriamin-N,N,N',N,N-pentaacetic acid, pentetic acid) is a chelator of transition metals, not cell membrane permeable. general chelates multivalent ions stronger than EDTA; pKa1 = 2.14, pKa2 = 2.38, pKa3 = 4.26 pKa4 = 8.60, pKa5 = 10.53
Abbreviation: DTPA
Description
- CAS: 67-43-6
- Source: EHRENSTORFER GMBH (EHERE13095000) or Sigma Aldrich (D-1133).
- FW: 393.35
- Solubility in H2O: ~ 4,8 g/l at 25 °C = 12 mM
- Stability constant: see: [1]:
Metal DTPA EDTA Ca(II) 10.74 10.96 Mg(II) 9.3 8.69 Fe(II) 16.5 14.3 Fe(III) 28.6 25.1 Mn(II) 15.6 14.04 Zn (II) 18.75 16.5
DTPA in O2k-FluoRespirometry
- Final concentration in O2k-chamber: 15 µM
- Stock solution: