Difference between revisions of "DTPA"

Revision as of 14:24, 22 August 2017

{{MitoPedia |abbr=DTPA |description=DTPA (Diethylentriamin-N,N,N',N,N-pentaacetic acid, pentetic acid,[[(Carboxymethyl)imino]bis(ethylenenitrilo)]-tetra-acetic acid) is a polyaminopolycarboxylic acid (like EDTA) chelator of transition metals, which as a form of calcium or zinc trisodium salt acts by exchanging calcium or zinc ions for a metal with a higher binding capacity. The compound is not cell membrane permeable. In general it chelates multivalent ions stronger than EDTA. The pKa1 = 2.14, pKa2 = 2.38, pKa3 = 4.26 pKa4 = 8.60, pKa5 = 10.53 }}

Description

CAS: 67-43-6
Source: EHRENSTORFER GMBH (EHERE13095000) or Sigma Aldrich (D-1133).
FW: 393.35
Chemical formula: C₂H₂O₂
Solubility: It is soluble in water (4,8 g/l at 25 °C), but not readily soluble in ethanol or apolar solvents.
Stability constant: see: [1]:

Metal	DTPA	EDTA
Ca(II)	10.74	10.96
Mg(II)	9.3	8.69
Fe(II)	16.5	14.3
Fe(III)	28.6	25.1
Mn(II)	15.6	14.04
Zn (II)	18.75	16.5

DTPA in HRFR

Fluorimetry:

Final concentration in O2k-chamber: 15 µM
Stock solution: 5 mM
Preparation of the 5 mM stock solution of DTPA:

Weigh 1.967 mg of DTPA in a 2 ml glass vial.
Dissolve in 500 ul ddH₂O and add 6 ul 5M KOH to set the pH.
Heat up the solution up to 33 °C.
Add 2 ul 5M KOH to adjust the pH~7.
Add 500 ul ddH₂O to get 5 mM stock solution.
The solution has to be prepared in glass vials, but after then it can be stored in Eppendorf tubes at -20 °C.

@@ Line 37: / Line 37: @@
 ::::* '''Stock solution:''' 5 mM
 ::::* '''Preparation of the 5 mM stock solution of DTPA:'''
+::::# Weigh 1.967 mg of DTPA in a 2 ml glass vial.
+::::# Dissolve in 500 ul ddH<sub>2</sub>O and add 6 ul 5M KOH to set the pH.
+::::# Heat up the solution up to 33 °C.
+::::# Add 2 ul 5M KOH to adjust the pH~7.
+::::# Add 500 ul ddH<sub>2</sub>O to get 5 mM stock solution.
+::::# The solution has to be prepared in glass vials, but after then it can be stored in Eppendorf tubes at -20 °C.