DTPA
From Bioblast
{{MitoPedia |abbr=DTPA |description=DTPA (Diethylentriamin-N,N,N',N,N-pentaacetic acid, pentetic acid,[[(Carboxymethyl)imino]bis(ethylenenitrilo)]-tetra-acetic acid) is a polyaminopolycarboxylic acid (like EDTA) chelator of transition metals, which as a form of calcium or zinc trisodium salt acts by exchanging calcium or zinc ions for a metal with a higher binding capacity. The compound is not cell membrane permeable. In general it chelates multivalent ions stronger than EDTA. The pKa1 = 2.14, pKa2 = 2.38, pKa3 = 4.26 pKa4 = 8.60, pKa5 = 10.53 }}
Description
- CAS: 67-43-6
- Source: EHRENSTORFER GMBH (EHERE13095000) or Sigma Aldrich (D-1133).
- FW: 393.35
- Chemical formula: C2H2O2
- Solubility: It is soluble in water (4,8 g/l at 25 °C), but not readily soluble in ethanol or apolar solvents.
- Stability constant: see: [1]:
Metal DTPA EDTA Ca(II) 10.74 10.96 Mg(II) 9.3 8.69 Fe(II) 16.5 14.3 Fe(III) 28.6 25.1 Mn(II) 15.6 14.04 Zn (II) 18.75 16.5
DTPA in HRFR
Fluorimetry:
- Final concentration in O2k-chamber: 15 µM
- Stock solution: 5 mM
- Preparation of the 5 mM stock solution of DTPA:
- Weigh 1.967 mg of DTPA in a 2 ml glass vial.
- Dissolve in 500 ul ddH2O and add 6 ul of 5M KOH to set the pH.
- Heat up the solution up to 33 °C.
- Add 2 ul 5M KOH to adjust the pH~7.
- Add 500 ul ddH2O to get 5 mM stock solution.
- The solution has to be prepared in glass vials, but after then it can be stored in Eppendorf tubes at -20 °C.
