Dejean 2000 Biochim Biophys Acta

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Dejean L, Beauvoit B, Guérin B, Rigoulet M (2000) Growth of the yeast Saccharomyces cerevisiae on a non-fermentable substrate: Control of energetic yield by the amount of mitochondria. Biochim Biophys Acta 1457:45-56.

» PMID: 10692549 Open Access

Dejean L, Beauvoit B, Guerin B, Rigoulet M (2000) Biochim Biophys Acta

Abstract: The purpose of this study was to investigate the long-term control of ATP synthesis during the course of Saccharomyces cerevisiae batch grown on lactate, a purely respiratory substrate. For this, we used a respirometric and on-line calorimetric approach to analyse the energetic balances and the control of energetic metabolism during growth. Enthalpic growth yields assessed by enthalpy balance (taking account of substrate consumption, by-product accumulation, biomass formation and heat dissipation) remained constant during the entire exponential growth. Moreover, at the same time, a parallel decrease in basal respiratory rate and enthalpy flux occurred. It is shown that the decrease in respiration corresponds to a decrease in the amount of mitochondria per cell but not to a change of steady state of oxidative phosphorylation. Taking into account the part of energy used for maintenance, it can be concluded that mitochondria by themselves are the major heat dissipative system in a fully aerobic metabolism, and that the decrease in the amount of mitochondria when growth rate decreases leads to an enthalpic growth yield constant.

Keywords: Yeast, Microcalorimetry, Enthalpic growth yield, Mitochondria, Oxidative phosphorylation

O2k-Network Lab: FR Pessac Diolez P, FR Bordeaux Devin A

Labels: MiParea: Respiration, mt-Biogenesis;mt-density 

Organism: Saccharomyces cerevisiae, Fungi 

Preparation: Intact cells 

Regulation: Aerobic glycolysis, ADP, Temperature  Coupling state: OXPHOS 

HRR: Oxygraph-2k