Difference between revisions of "Di Marcello 2019 MitoFit Preprint Arch EA"

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|mipnetlab=AT Innsbruck Oroboros, AT Innsbruck Gnaiger E
 
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== Affiliations ==
 
== Affiliations ==
 
Di Marcello M(1), Iglesias-Gonzalez J(1), Meszaros A(1,2), Haider M(3), Gnaiger E(1,2), Huete-Ortega M(1)
 
Di Marcello M(1), Iglesias-Gonzalez J(1), Meszaros A(1,2), Haider M(3), Gnaiger E(1,2), Huete-Ortega M(1)
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::::# D Swarovski Research Lab, Dept Visceral, Transplant Thoracic Surgery, Med Univ Innsbruck, Austria
 
::::# D Swarovski Research Lab, Dept Visceral, Transplant Thoracic Surgery, Med Univ Innsbruck, Austria
 
::::# SHTech, Innsbruck, Austria
 
::::# SHTech, Innsbruck, Austria
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== References ==
 +
:::# Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. - [[Doerrier 2018 Methods Mol Biol |»Bioblast link«]]
 +
:::# Young L, Shiba T, Harada S, Kita K, Albury MS, Moore AL (2013) The alternative oxidases: simple oxidoreductase proteins with complex functions. Biochem Soc Trans 41:1305-11. - [[Young 2013 Biochem Soc Trans |»Bioblast link«]]
 +
:::# [[SUIT-022_O2_ce_D051 |www.bioblast.at/index.php/SUIT-022_O2_ce_D051]]
 +
:::# [[SUIT-023_O2_ce_D053 |www.bioblast.at/index.php/SUIT-023_O2_ce_D053]]
 +
:::# Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128:277-97. - [[Gnaiger 2001 Respir Physiol |»Bioblast link«]]
 +
:::# Meszaros AT, Haider M, Di Marcello M, Gnaiger E (2018) High-resolution mitochondrial oxygen kinetics as diagnostic tool in Complex IV impairments. Abstract Mitochondrial Medicine 2018 Hinxton UK. -  - [[Meszaros_2018_Mt_Med_Hinxton |»Bioblast link«]]
 +
:::# Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662:7-25. - [[Scandurra 2010 Adv Exp Med Biol |»Bioblast link«]]
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Revision as of 06:59, 16 June 2019

Publications in the MiPMap
Di Marcello M, Iglesias-Gonzalez J, Meszaros A, Haider M, Gnaiger E, Huete-Ortega M (2019) Partitioning between cytochrome c oxidase and alternative oxidase studied by oxygen kinetics of dark respiration in Chlamydomonas reinhardtii: a microalgae model organism. MitoFit Preprint Arch doi:10.26124/mitofit:ea19.MiPSchool.0005.

»

MitoFit pdf

Partitioning between cytochrome c oxidase and alternative oxidase studied by oxygen kinetics of dark respiration in Chlamydomonas reinhardtii: a microalgae model organism

Di Marcello M, Iglesias-Gonzalez J, Meszaros A, Haider M, Gnaiger E, Huete-Ortega M (2019) MitoFit Preprint Arch

Abstract: Version 1 (v1) 2019-06-15 doi:10.26124/mitofit:ea19.MiPSchool.0005 (in preparation)

Bioenergetics is the study of how living organisms acquire and transform energy to perform biological work. Energetic coupling between chloroplasts and mitochondria has been described in algae, demonstrating the a good functionality and interaction between both organelles is necessary to maintain metabolic integrity. High-resolution respirometry (HRR) is widely used to assess mitochondrial respiration and other bioenergetics parameters in the biomedical field of mitochondrial research and its clinical applications. In our interdisciplinary study, we adapted the multimodal approach of the Oroboros O2k high-resolution respirometer to investigate algal bioenergetics for biotechnological purposes. - Extended abstract

Keywords: AOX Bioblast editor: Gnaiger E O2k-Network Lab: AT Innsbruck Oroboros, AT Innsbruck Gnaiger E

Template NextGen-O2k.jpg

Affiliations

Di Marcello M(1), Iglesias-Gonzalez J(1), Meszaros A(1,2), Haider M(3), Gnaiger E(1,2), Huete-Ortega M(1)

  1. Oroboros Instruments, Innsbruck, Austria - marco.dimarcello@oroboros.at
  2. D Swarovski Research Lab, Dept Visceral, Transplant Thoracic Surgery, Med Univ Innsbruck, Austria
  3. SHTech, Innsbruck, Austria

References

  1. Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. - »Bioblast link«
  2. Young L, Shiba T, Harada S, Kita K, Albury MS, Moore AL (2013) The alternative oxidases: simple oxidoreductase proteins with complex functions. Biochem Soc Trans 41:1305-11. - »Bioblast link«
  3. www.bioblast.at/index.php/SUIT-022_O2_ce_D051
  4. www.bioblast.at/index.php/SUIT-023_O2_ce_D053
  5. Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128:277-97. - »Bioblast link«
  6. Meszaros AT, Haider M, Di Marcello M, Gnaiger E (2018) High-resolution mitochondrial oxygen kinetics as diagnostic tool in Complex IV impairments. Abstract Mitochondrial Medicine 2018 Hinxton UK. - - »Bioblast link«
  7. Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662:7-25. - »Bioblast link«


Labels: MiParea: Respiration, Instruments;methods, Comparative MiP;environmental MiP 


Organism: Plants 

Preparation: Intact cells  Enzyme: Complex IV;cytochrome c oxidase  Regulation: Oxygen kinetics  Coupling state: ROUTINE 

HRR: Oxygraph-2k