Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Dihydroethidium

From Bioblast
Revision as of 12:46, 3 August 2020 by Cardoso Luiza (talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision β†’ (diff)


high-resolution terminology - matching measurements at high-resolution


Dihydroethidium

Description

Dihydroethidium (also called hydroethidine) is a cell permeant fluorescent probe used to analyse superoxide presence. It is a reduced form of ethidium that presents blue fluorescence, and after oxidation by superoxide becomes able to intercalate DNA and emits red fluorescence (excitation wavelength ~518–535 nm, emission ~605–610 nm). It has been used to detect superoxide by HPLC and by fluorescence microscopy.

Abbreviation: DHE

Reference: Laurindo 2008 Methods Enzymol, Kalivendi 2003 Biochem J