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Dithionite

From Bioblast


high-resolution terminology - matching measurements at high-resolution


Dithionite

Description

Zero oxygen solution powder, Na2S2O4, used for calibration of oxygen sensors at zero oxygen, or for stepwise reduction of oxygen concentration in instrumental O2 background tests.

Abbreviation: Dit

Reference: MiPNet06.03 POS-calibration-SOP, MiPNet14.06 Instrumental O2 background



MitoPedia methods: Respirometry 




Preparation

Preparation of 30 mM dithionite solution for instrumental oxygen background (dissolved in Phosphate buffer, 50 mM, pH 8)
  1. Weigh 0.051 g dithionite, sodium hydrosulfite, and transfer to 10 mL volumetric glass flask.
  2. Adjust final volume to 10 mL with Phosphate buffer and keep closed to avoid decomposing.
Preparation of 10 mM dithionite solution for instrumental oxygen background (dissolved in Phosphate buffer, 50 mM, pH 8)
  1. Weigh 0.017 g dithionite, sodium hydrosulfite, and transfer to 10 mL volumetric glass flask.
  2. Adjust final volume to 10 mL with Phosphate buffer and keep closed to avoid decomposing.
Note: Experiments performed with 10 and 30 mM dithionite solution showed identical results. However, sodium dithionite is slowly oxidized by air. When dithionite is open for long time, we recommend to use a concentration of 30 mM for performing the instrumental oxygen background. For new dithionite, 10 mM dithionite solution may be used.


Further information Β»Talk:Dithionite

Template NextGen-O2k.jpg


MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



O2k open support cases

Template:OSC Dithionite

Anonymous user

Question:

Q1: The Oxygen Concentration couldn't go down while I tried zero oxygen calibration. No matter how much solution I added, the oxygen concentration still remain the same. I reassembled, but it still keeps the same. The data is attached.

No response to dithionite.PNG


Q2: The chamber B's oxygen slope is not stable. This is a new problem I have never seen. Please see the attachment.(2019-04-26)

Signal stability.PNG


Q3. The maximum Oxygen concentration of another Oroboros O2k is only about 140 ΞΌM. I don't how to increase the starting point of oxygen concentration.


Answer:

A1: I suspect that the dithionite solution was not correctly prepared or not fresh. Dithionite loses activity quite fast, so you need to make it freshly. Please check this site: http://bioblast.at/index.php/Dithionite

A2: While there is a difference between chambers A and B, the stability of the signal is still within the acceptable range of noise given by the specifications of the instrument, see: Oxygen_sensor_test.

A3: You may simply need to calibrate, please see here: http://wiki.oroboros.at/index.php/O2_calibration_-_DatLab


MitoPedia O2k and high-resolution respirometry: O2k-Open Support