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Doerrier 2018 Methods Mol Biol - Revision history
2024-03-29T06:02:14Z
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Gnaiger Erich at 10:27, 5 March 2024
2024-03-05T10:27:22Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 10:27, 5 March 2024</td>
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<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|title=Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. https://doi.org/10.1007/978-1-4939-7831-1_3</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|title=Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria<ins style="font-weight: bold; text-decoration: none;">. Methods Mol Biol 1782:31-70</ins>. https://doi.org/10.1007/978-1-4939-7831-1_3</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|info=<del style="font-weight: bold; text-decoration: none;">Methods Mol Biol 1782:31-70. </del>[https://www.ncbi.nlm.nih.gov/pubmed/29850993 PMID: 29850993] »[[File:O2k-brief.png|36px|link=http://wiki.oroboros.at/images/4/46/Doerrier_2018_Methods_Mol_Biol_O2k-brief.pdf|O2k-brief]][[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|info=[https://www.ncbi.nlm.nih.gov/pubmed/29850993 PMID: 29850993] »[[File:O2k-brief.png|36px|link=http://wiki.oroboros.at/images/4/46/Doerrier_2018_Methods_Mol_Biol_O2k-brief.pdf|O2k-brief]][[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] </div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|authors=Doerrier Carolina, Garcia-Souza Luiz F, Krumschnabel Gerhard, Wohlfarter Yvonne, Meszaros Andras T, Gnaiger Erich</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|authors=Doerrier Carolina, Garcia-Souza Luiz F, Krumschnabel Gerhard, Wohlfarter Yvonne, Meszaros Andras T, Gnaiger Erich</div></td></tr>
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Gnaiger Erich
https://wiki.oroboros.at/index.php?title=Doerrier_2018_Methods_Mol_Biol&diff=246158&oldid=prev
Gnaiger Erich at 07:56, 23 February 2024
2024-02-23T07:56:36Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 07:56, 23 February 2024</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|info=Methods Mol Biol 1782:31-70. [https://www.ncbi.nlm.nih.gov/pubmed/29850993 PMID: 29850993] »[[File:O2k-brief.png|36px|link=http://wiki.oroboros.at/images/4/46/Doerrier_2018_Methods_Mol_Biol_O2k-brief.pdf|O2k-brief]][[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|info=Methods Mol Biol 1782:31-70. [https://www.ncbi.nlm.nih.gov/pubmed/29850993 PMID: 29850993] »[[File:O2k-brief.png|36px|link=http://wiki.oroboros.at/images/4/46/Doerrier_2018_Methods_Mol_Biol_O2k-brief.pdf|O2k-brief]][[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] </div></td></tr>
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Gnaiger Erich
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Gnaiger Erich at 12:55, 4 June 2022
2022-06-04T12:55:12Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 12:55, 4 June 2022</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|title=Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. https://doi.org/10.1007/978-1-4939-7831-1_3</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|title=Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. https://doi.org/10.1007/978-1-4939-7831-1_3</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|info=[https://www.ncbi.nlm.nih.gov/pubmed/29850993 PMID: 29850993] <del style="font-weight: bold; text-decoration: none;">Methods Mol Biol 1782:31-70. </del>»[[File:O2k-brief.png|36px|link=http://wiki.oroboros.at/images/4/46/Doerrier_2018_Methods_Mol_Biol_O2k-brief.pdf|O2k-brief]][[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|info=<ins style="font-weight: bold; text-decoration: none;">Methods Mol Biol 1782:31-70. </ins>[https://www.ncbi.nlm.nih.gov/pubmed/29850993 PMID: 29850993] »[[File:O2k-brief.png|36px|link=http://wiki.oroboros.at/images/4/46/Doerrier_2018_Methods_Mol_Biol_O2k-brief.pdf|O2k-brief]][[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] </div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|authors=Doerrier Carolina, Garcia-Souza Luiz F, Krumschnabel Gerhard, Wohlfarter Yvonne, Meszaros Andras, Gnaiger Erich</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|authors=Doerrier Carolina, Garcia-Souza Luiz F, Krumschnabel Gerhard, Wohlfarter Yvonne, Meszaros Andras, Gnaiger Erich</div></td></tr>
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Gnaiger Erich
https://wiki.oroboros.at/index.php?title=Doerrier_2018_Methods_Mol_Biol&diff=228261&oldid=prev
Gnaiger Erich at 12:53, 4 June 2022
2022-06-04T12:53:04Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 12:53, 4 June 2022</td>
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<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|title=Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. <del style="font-weight: bold; text-decoration: none;">Methods Mol Biol 1782</del>:<del style="font-weight: bold; text-decoration: none;">31-70</del>. <del style="font-weight: bold; text-decoration: none;">doi: </del>10.1007/978-1-4939-7831-1_3</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|title=Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. <ins style="font-weight: bold; text-decoration: none;">https</ins>:<ins style="font-weight: bold; text-decoration: none;">//doi</ins>.<ins style="font-weight: bold; text-decoration: none;">org/</ins>10.1007/978-1-4939-7831-1_3</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|info=[https://www.ncbi.nlm.nih.gov/pubmed/29850993 PMID: 29850993] »[[File:O2k-brief.png|36px|link=http://wiki.oroboros.at/images/4/46/Doerrier_2018_Methods_Mol_Biol_O2k-brief.pdf|O2k-brief]][[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]]</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|info=[https://www.ncbi.nlm.nih.gov/pubmed/29850993 PMID: 29850993] <ins style="font-weight: bold; text-decoration: none;">Methods Mol Biol 1782:31-70. </ins>»[[File:O2k-brief.png|36px|link=http://wiki.oroboros.at/images/4/46/Doerrier_2018_Methods_Mol_Biol_O2k-brief.pdf|O2k-brief]][[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] </div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|authors=Doerrier Carolina, Garcia-Souza Luiz F, Krumschnabel Gerhard, Wohlfarter Yvonne, Meszaros Andras, Gnaiger Erich</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|authors=Doerrier Carolina, Garcia-Souza Luiz F, Krumschnabel Gerhard, Wohlfarter Yvonne, Meszaros Andras, Gnaiger Erich</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|year=2018</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|year=2018</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|journal=Methods Mol Biol</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|journal=Methods Mol Biol</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|abstract=Protocols for high-resolution respirometry of intact cells, permeabilized cells, permeabilized muscle fibers, isolated mitochondria and tissue homogenates offer sensitive diagnostic tests of integrated mitochondrial function using standard cell culture techniques, small needle biopsies of muscle, and mitochondrial preparation methods. Multiple substrate-uncoupler-inhibitor titration (SUIT) protocols for analysis of oxidative phosphorylation (OXPHOS) improve our understanding of mitochondrial respiratory control and the pathophysiology of mitochondrial diseases. Respiratory states are defined in functional terms to account for the network of metabolic interactions in complex SUIT protocols with stepwise modulation of coupling control and electron transfer pathway states. A regulated degree of intrinsic uncoupling is a hallmark of oxidative phosphorylation, whereas pathological and toxicological dyscoupling is evaluated as a mitochondrial defect. The noncoupled state of maximum respiration is experimentally induced by titration of established uncouplers (CCCP, FCCP, DNP), to collapse the protonmotive force across the mitochondrial inner membrane and measure the electron transfer capacity (ET; open-circuit operation of respiration). Intrinsic uncoupling and dyscoupling are evaluated as the flux control ratio between non-phosphorylating LEAK respiration (electron flow coupled to proton pumping to compensate for proton leaks) and ET capacity. If OXPHOS capacity (maximally ADP stimulated O2 flux) is less than ET capacity, the phosphorylation pathway contributes to flux control. Physiological substrate combinations supporting the NADH&succinate-pathway are required to reconstitute tricarboxylic acid cycle function. This supports maximum ET and OXPHOS capacities, due to the additive effect of multiple electron supply pathways converging at the Q-junction. ET-pathways with electron entry separately through NADH (pyruvate&malate or glutamate&malate) or succinate (succinate&rotenone) restricts ET capacity and artificially enhances flux control upstream of the Q-cycle, providing diagnostic information on specific ET-pathway branches. O2 concentration is maintained above air saturation in protocols with permeabilized muscle fibers to avoid experimental O2 limitation of respiration. Standardized two-point calibration of the polarographic oxygen sensor (static sensor calibration), calibration of the sensor response time (dynamic sensor calibration), and evaluation of instrumental background O2 flux (systemic flux compensation) provide the unique experimental basis for high accuracy of quantitative results and quality control in high-resolution respirometry.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|abstract=Protocols for high-resolution respirometry of intact cells, permeabilized cells, permeabilized muscle fibers, isolated mitochondria and tissue homogenates offer sensitive diagnostic tests of integrated mitochondrial function using standard cell culture techniques, small needle biopsies of muscle, and mitochondrial preparation methods. Multiple substrate-uncoupler-inhibitor titration (SUIT) protocols for analysis of oxidative phosphorylation (OXPHOS) improve our understanding of mitochondrial respiratory control and the pathophysiology of mitochondrial diseases. Respiratory states are defined in functional terms to account for the network of metabolic interactions in complex SUIT protocols with stepwise modulation of coupling control and electron transfer pathway states. A regulated degree of intrinsic uncoupling is a hallmark of oxidative phosphorylation, whereas pathological and toxicological dyscoupling is evaluated as a mitochondrial defect. The noncoupled state of maximum respiration is experimentally induced by titration of established uncouplers (CCCP, FCCP, DNP), to collapse the protonmotive force across the mitochondrial inner membrane and measure the electron transfer capacity (ET; open-circuit operation of respiration). Intrinsic uncoupling and dyscoupling are evaluated as the flux control ratio between non-phosphorylating LEAK respiration (electron flow coupled to proton pumping to compensate for proton leaks) and ET capacity. If OXPHOS capacity (maximally ADP stimulated O2 flux) is less than ET capacity, the phosphorylation pathway contributes to flux control. Physiological substrate combinations supporting the NADH&succinate-pathway are required to reconstitute tricarboxylic acid cycle function. This supports maximum ET and OXPHOS capacities, due to the additive effect of multiple electron supply pathways converging at the Q-junction. ET-pathways with electron entry separately through NADH (pyruvate&malate or glutamate&malate) or succinate (succinate&rotenone) restricts ET capacity and artificially enhances flux control upstream of the Q-cycle, providing diagnostic information on specific ET-pathway branches. O2 concentration is maintained above air saturation in protocols with permeabilized muscle fibers to avoid experimental O2 limitation of respiration. Standardized two-point calibration of the polarographic oxygen sensor (static sensor calibration), calibration of the sensor response time (dynamic sensor calibration), and evaluation of instrumental background O2 flux (systemic flux compensation) provide the unique experimental basis for high accuracy of quantitative results and quality control in high-resolution respirometry.</div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|editor=[[Gnaiger E]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|editor=[[Gnaiger E]]</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck Gnaiger E, AT Innsbruck Oroboros</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck Gnaiger E, AT Innsbruck Oroboros</div></td></tr>
</table>
Gnaiger Erich
https://wiki.oroboros.at/index.php?title=Doerrier_2018_Methods_Mol_Biol&diff=226910&oldid=prev
Gnaiger Erich at 08:29, 5 May 2022
2022-05-05T08:29:25Z
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|instruments=Oxygraph-2k, TIP2k, O2k-Protocol</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|instruments=Oxygraph-2k, TIP2k, O2k-Protocol</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|additional=SUIT-001, SUIT-001 O2 mt D001, SUIT-001 O2 pfi D002, SUIT-001 O2 ce-pce D003, SUIT-001 O2 ce-pce D004, SUIT-002, SUIT-002 O2 mt D005, SUIT-002 O2 pfi D006, SUIT-002 O2 ce-pce D007, SUIT-002 O2 ce-pce D007a, SUIT-003 O2 ce-pce D020, SUIT-010, SUIT-010 O2 pce D008, SUIT-010, SUIT-010 O2 ce-pce D050, SUIT-010 O2 ce-pce D008, MitoPathways, MitoFitPublication, MitoEAGLEPublication, O2k-chemicals and media, MitoEAGLE blood cells reviews, O2k-brief, Flux control ratio, BEC 2020.1, BEC 2020.2, MitoFit 2021 MgG, MitoFit 2021 CoQ, MitoFit 2021.5 PB, MitoFit 2021 AmR-O2, MitoFit 2021 AmR, MitoFit 2021 Tissue normoxia, MitoFit 2022 NADH, MitoFit 2021 BCA, MitoFit 2021 PLT, PLoSONE2022ace-sce</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|additional=SUIT-001, SUIT-001 O2 mt D001, SUIT-001 O2 pfi D002, SUIT-001 O2 ce-pce D003, SUIT-001 O2 ce-pce D004, SUIT-002, SUIT-002 O2 mt D005, SUIT-002 O2 pfi D006, SUIT-002 O2 ce-pce D007, SUIT-002 O2 ce-pce D007a, SUIT-003 O2 ce-pce D020, SUIT-010, SUIT-010 O2 pce D008, SUIT-010, SUIT-010 O2 ce-pce D050, SUIT-010 O2 ce-pce D008, MitoPathways, MitoFitPublication, MitoEAGLEPublication, O2k-chemicals and media, MitoEAGLE blood cells reviews, O2k-brief, Flux control ratio, BEC 2020.1, BEC 2020.2, MitoFit 2021 MgG, MitoFit 2021 CoQ, MitoFit 2021.5 PB, MitoFit 2021 AmR-O2, MitoFit 2021 AmR, MitoFit 2021 Tissue normoxia, MitoFit 2022 NADH, MitoFit 2021 BCA, MitoFit 2021 PLT, PLoSONE2022ace-sce<ins style="font-weight: bold; text-decoration: none;">, MitoFit2022QC</ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td></tr>
</table>
Gnaiger Erich
https://wiki.oroboros.at/index.php?title=Doerrier_2018_Methods_Mol_Biol&diff=226789&oldid=prev
Gnaiger Erich at 10:16, 3 May 2022
2022-05-03T10:16:02Z
<p></p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 10:16, 3 May 2022</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Template:Cited by Gnaiger 2021 MitoFit BCA}}</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Template:Cited by Gnaiger 2021 MitoFit BCA}}</div></td></tr>
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</table>
Gnaiger Erich
https://wiki.oroboros.at/index.php?title=Doerrier_2018_Methods_Mol_Biol&diff=225099&oldid=prev
Gnaiger Erich at 22:37, 4 March 2022
2022-03-04T22:37:10Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 22:37, 4 March 2022</td>
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<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|additional=SUIT-001, SUIT-001 O2 mt D001, SUIT-001 O2 pfi D002, SUIT-001 O2 ce-pce D003, SUIT-001 O2 ce-pce D004, SUIT-002, SUIT-002 O2 mt D005, SUIT-002 O2 pfi D006, SUIT-002 O2 ce-pce D007, SUIT-002 O2 ce-pce D007a, SUIT-003 O2 ce-pce D020, SUIT-010, SUIT-010 O2 pce D008, SUIT-010, SUIT-010 O2 ce-pce D050, SUIT-010 O2 ce-pce D008, MitoPathways, MitoFitPublication, MitoEAGLEPublication, O2k-chemicals and media, MitoEAGLE blood cells reviews, O2k-brief, Flux control ratio, BEC 2020.1, BEC 2020.2, MitoFit 2021 MgG, MitoFit 2021 CoQ, MitoFit 2021.5 PB, MitoFit 2021 AmR-O2, MitoFit 2021 AmR, MitoFit 2021 Tissue normoxia, MitoFit 2022 NADH, MitoFit 2021 BCA, MitoFit 2021 PLT</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|additional=SUIT-001, SUIT-001 O2 mt D001, SUIT-001 O2 pfi D002, SUIT-001 O2 ce-pce D003, SUIT-001 O2 ce-pce D004, SUIT-002, SUIT-002 O2 mt D005, SUIT-002 O2 pfi D006, SUIT-002 O2 ce-pce D007, SUIT-002 O2 ce-pce D007a, SUIT-003 O2 ce-pce D020, SUIT-010, SUIT-010 O2 pce D008, SUIT-010, SUIT-010 O2 ce-pce D050, SUIT-010 O2 ce-pce D008, MitoPathways, MitoFitPublication, MitoEAGLEPublication, O2k-chemicals and media, MitoEAGLE blood cells reviews, O2k-brief, Flux control ratio, BEC 2020.1, BEC 2020.2, MitoFit 2021 MgG, MitoFit 2021 CoQ, MitoFit 2021.5 PB, MitoFit 2021 AmR-O2, MitoFit 2021 AmR, MitoFit 2021 Tissue normoxia, MitoFit 2022 NADH, MitoFit 2021 BCA, MitoFit 2021 PLT<ins style="font-weight: bold; text-decoration: none;">, PLoSONE2022ace-sce</ins></div></td></tr>
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</table>
Gnaiger Erich
https://wiki.oroboros.at/index.php?title=Doerrier_2018_Methods_Mol_Biol&diff=224105&oldid=prev
Doerrier Carolina at 15:45, 31 January 2022
2022-01-31T15:45:09Z
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|instruments=Oxygraph-2k, TIP2k, O2k-Protocol</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|instruments=Oxygraph-2k, TIP2k, O2k-Protocol</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|additional=SUIT-001, SUIT-001 O2 mt D001, SUIT-001 O2 pfi D002, SUIT-001 O2 ce-pce D003, SUIT-001 O2 ce-pce D004, SUIT-002, SUIT-002 O2 mt D005, SUIT-002 O2 pfi D006, SUIT-002 O2 ce-pce D007, SUIT-002 O2 ce-pce D007a, SUIT-003 O2 ce-pce D020, SUIT-010, SUIT-010 O2 pce D008, SUIT-010, SUIT-010 O2 ce-pce D050, SUIT-010 O2 ce-pce D008, MitoPathways, MitoFitPublication, MitoEAGLEPublication, O2k-chemicals and media, MitoEAGLE blood cells reviews, O2k-brief, Flux control ratio, BEC 2020.1, BEC 2020.2, MitoFit 2021 MgG, MitoFit 2021 CoQ, MitoFit 2021.5 PB, MitoFit 2021 AmR-O2, MitoFit 2021 AmR, MitoFit 2021 Tissue normoxia, MitoFit <del style="font-weight: bold; text-decoration: none;">2021 </del>NADH, MitoFit 2021 BCA, MitoFit 2021 PLT</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|additional=SUIT-001, SUIT-001 O2 mt D001, SUIT-001 O2 pfi D002, SUIT-001 O2 ce-pce D003, SUIT-001 O2 ce-pce D004, SUIT-002, SUIT-002 O2 mt D005, SUIT-002 O2 pfi D006, SUIT-002 O2 ce-pce D007, SUIT-002 O2 ce-pce D007a, SUIT-003 O2 ce-pce D020, SUIT-010, SUIT-010 O2 pce D008, SUIT-010, SUIT-010 O2 ce-pce D050, SUIT-010 O2 ce-pce D008, MitoPathways, MitoFitPublication, MitoEAGLEPublication, O2k-chemicals and media, MitoEAGLE blood cells reviews, O2k-brief, Flux control ratio, BEC 2020.1, BEC 2020.2, MitoFit 2021 MgG, MitoFit 2021 CoQ, MitoFit 2021.5 PB, MitoFit 2021 AmR-O2, MitoFit 2021 AmR, MitoFit 2021 Tissue normoxia, MitoFit <ins style="font-weight: bold; text-decoration: none;">2022 </ins>NADH, MitoFit 2021 BCA, MitoFit 2021 PLT</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td></tr>
</table>
Doerrier Carolina
https://wiki.oroboros.at/index.php?title=Doerrier_2018_Methods_Mol_Biol&diff=223129&oldid=prev
Gnaiger Erich at 10:37, 27 December 2021
2021-12-27T10:37:57Z
<p></p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 10:37, 27 December 2021</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Cited by ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Cited by ==</div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">::* 37 articles in PubMed (2021-12-27) https://pubmed.ncbi.nlm.nih.gov/29850993/</ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Template:Cited by Gnaiger 2021 MitoFit BCA}}</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Template:Cited by Gnaiger 2021 MitoFit BCA}}</div></td></tr>
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</table>
Gnaiger Erich
https://wiki.oroboros.at/index.php?title=Doerrier_2018_Methods_Mol_Biol&diff=220707&oldid=prev
Gnaiger Erich at 19:45, 24 September 2021
2021-09-24T19:45:45Z
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Template:Cited by Silva 2021 MitoFit Etomoxir}}</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Template:Cited by Silva 2021 MitoFit Etomoxir}}</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|instruments=Oxygraph-2k, TIP2k, O2k-Protocol</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|instruments=Oxygraph-2k, TIP2k, O2k-Protocol</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|additional=SUIT-001, SUIT-001 O2 mt D001, SUIT-001 O2 pfi D002, SUIT-001 O2 ce-pce D003, SUIT-001 O2 ce-pce D004, SUIT-002, SUIT-002 O2 mt D005, SUIT-002 O2 pfi D006, SUIT-002 O2 ce-pce D007, SUIT-002 O2 ce-pce D007a, SUIT-003 O2 ce-pce D020, SUIT-010, SUIT-010 O2 pce D008, SUIT-010, SUIT-010 O2 ce-pce D050, SUIT-010 O2 ce-pce D008, MitoPathways, MitoFitPublication, MitoEAGLEPublication, O2k-chemicals and media, MitoEAGLE blood cells reviews, O2k-brief, Flux control ratio, BEC 2020.1, BEC 2020.2, MitoFit 2021 MgG, MitoFit 2021 CoQ, MitoFit 2021.5 PB, MitoFit 2021 <del style="font-weight: bold; text-decoration: none;">Yeast</del>, MitoFit 2021 AmR, MitoFit 2021 Tissue normoxia, MitoFit 2021 NADH, MitoFit 2021 BCA, MitoFit 2021 PLT</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|additional=SUIT-001, SUIT-001 O2 mt D001, SUIT-001 O2 pfi D002, SUIT-001 O2 ce-pce D003, SUIT-001 O2 ce-pce D004, SUIT-002, SUIT-002 O2 mt D005, SUIT-002 O2 pfi D006, SUIT-002 O2 ce-pce D007, SUIT-002 O2 ce-pce D007a, SUIT-003 O2 ce-pce D020, SUIT-010, SUIT-010 O2 pce D008, SUIT-010, SUIT-010 O2 ce-pce D050, SUIT-010 O2 ce-pce D008, MitoPathways, MitoFitPublication, MitoEAGLEPublication, O2k-chemicals and media, MitoEAGLE blood cells reviews, O2k-brief, Flux control ratio, BEC 2020.1, BEC 2020.2, MitoFit 2021 MgG, MitoFit 2021 CoQ, MitoFit 2021.5 PB, MitoFit 2021 <ins style="font-weight: bold; text-decoration: none;">AmR-O2</ins>, MitoFit 2021 AmR, MitoFit 2021 Tissue normoxia, MitoFit 2021 NADH, MitoFit 2021 BCA, MitoFit 2021 PLT</div></td></tr>
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Gnaiger Erich