Drehmer 2016 Stem Cell Res

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Drehmer DL, de Aguiar AM, Brandt AP, Petiz L, Cadena SM, Rebelatto CK, Brofman PR, Filipak Neto F, Dallagiovanna B, Abud AP (2016) Metabolic switches during the first steps of adipogenic stem cells differentiation. Stem Cell Res 17:413-421.

» PMID: 27653462

Drehmer DL, de Aguiar AM, Brandt AP, Petiz L, Cadena SM, Rebelatto CK, Brofman PR, Filipak Neto F, Dallagiovanna B, Abud AP (2016) Stem Cell Res

Abstract: The understanding of metabolism during cell proliferation and commitment provides a greater insight into the basic biology of cells, allowing future applications. Here we evaluated the energy and oxidative changes during the early adipogenic differentiation of human adipose tissue-derived stromal cells (hASCs). hASCs were maintained under differentiation conditions during 3 and 7days. Oxygen consumption, mitochondrial mass and membrane potential, reactive oxygen species (ROS) generation, superoxide dismutase (SOD) and catalase activities, non-protein thiols (NPT) concentration and lipid peroxidation were analyzed. We observed that 7days of adipogenic induction are required to stimulate cells to consume more oxygen and increase mitochondrial activity, indicating organelle maturation and a transition from glycolytic to oxidative energy metabolism. ROS production was only increased after 3days and may be involved in the differentiation commitment. ROS source was not only the mitochondria and we suggest that NOX proteins are related to ROS generation and therefore adipogenic commitment. ROS production did not change after 7days, but an increased activity of catalase and NPT concentration as well as a decreased lipid peroxidation were observed. Thus, a short period of differentiation induction is able to change the energetic and oxidative metabolic profile of hASCs and stimulate cytoprotection processes.

Copyright © 2016. Published by Elsevier B.V.

Keywords: Adipogenesis, Adult stem cells, Antioxidant activity, Cytoprotection, Metabolism, Reactive oxygen species (ROS)


Labels: MiParea: Respiration 


Organism: Human  Tissue;cell: Fat, Stem cells  Preparation: Intact cells 


Coupling state: LEAK, ROUTINE, ET  Pathway: ROX  HRR: Oxygraph-2k 

2016-11