Fernandez-Ortiz Marisol

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BEC 2020.1 Mitochondrial physiology
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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping

Fernandez-Ortiz Marisol

MitoPedia topics: EAGLE 



Name Fernandez-Ortiz Marisol, PhD student
Institution Biomedical Research Center
Fernandez Ortiz M.jpg

University of Granada Parque Tecnológico de Ciencias de la Salud

Visiting scientist

  • Harmonization of respirometric protocols comparing isolated mitochondria and permeabilized muscle fibers

Marisol Fernandez-Ortiz was a visiting scientist at Oroboros Instruments from October to November 2017.

Address Avenida del Conocimiento, s/n, 18100
City Armilla (Granada)
Country Spain
Email marisol.fdez.ortiz@gmail.com
O2k-Network Lab ES Granada Acuna-Castroviejo D

MitoEAGLE Short-Term Scientific Mission

Motivation letter
My name is Marisol Fernández Ortiz and I completed a Master of Molecular Biology applied to Biotechnology Companies (BioEnterprise) at the University of Granada with the maximal qualification of High Honors last year. Prior to this, I studied the Degree in Biochemistry at the same University. In this period, I had the opportunity to collaborate with the Research Group ‘Biomembranes CTS-236’ in the Department of Biochemistry and Molecular Biology I of the University of Granada. Here, I acquired knowledge in the field of antitumor compounds through a Contract of Practice and a Fellowship of Introduction to Research within the Project of Excellence entitled ‘Development and evaluation of new antitumor alkylphospholipids that modify intracellular cholesterol homeostasis’. This whole experience was very useful to join to the Research Group ‘CTS-101: Intercellular Communication’, of which I am a member since January of 2016 and where I am currently performing my PhD under the supervision of the Prof. Dario Acuña Castroviejo. My doctoral thesis is supported by a FPU fellowship granted by Spanish Ministry of Economy and Competitiveness, and focused on the study of the mechanism of sarcopenia during aging and the relationship between chronodisruption, melatonin deficit, innate immunity and mitochondrial failure in skeletal and myocardial muscle during aging in mice.
The applied STSM MitoEAGLE is expected to be a great opportunity to learn new techniques that will be essential for my work. It will be performed according to the MitoFit Coaching Days Innsbruck. Oroboros-team will provide training for quality control in high-resolution respirometry, aimed at standardization of SUIT protocols and fostering proficiency to generate experimental data of assured high quality as required for a data base on mitochondrial physiology. This will highly contribute to the scientific objectives of MitoEAGLE regarding to Research Coordination and Capacity Building. Specifically, the proposed activities will intensify the dissemination of updated knowledge and know-how among partners and improve the collaborative relationships among the participating groups of the Action. The STSM will aid in the standardization and harmonization of protocols that increase the value and reduce the noise in mitochondrial research. The performed experiments will also generate experimental data of assured high quality, as required for a data base on mitochondrial physiology, and provide practical guidelines for students, scientist and stakeholders.
STSM request: download pdf

Participated at

Visiting scientist in the Oroboros O2k-Laboratory


Marisol Fernandez-Ortiz: Visiting scientist at the Oroboros O2k-Laboratory

  • October 01 to November 12 2017

Labels: Field of research: Basic 


BEC 2020.1 doi10.26124bec2020-0001.v12020Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1
Chabi 2019 MitoFit Preprint Arch EA2019Chabi Béatrice, Ost M, Gama-Perez P, Dahdah N, Lemieux H, Holody CD, Carpenter RG, Tepp K, Puurand M, Kaambre T, Dubouchaud H, Cortade F, Pesta D, Calabria E, Casado M, Fernandez-Ortiz M, Acuña-Castroviejo D, Villena JA, Grefte S, Keijer J, O'Brien K, Sowton A, Murray AJ, Campbell MD, Marcinek DJ, Nollet E, Wüst R, Dayanidhi S, Gnaiger E, Doerrier C, Garcia-Roves PM (2019) Generating reference values on mitochondrial respiration in permeabilized muscle fibers. https://doi.org/10.26124/mitofit:ea19.MitoEAGLE.0001
Lopez 2017 PLOS ONE2017López A, Ortiz F, Doerrier C, Venegas C, Fernández-Ortiz M, Aranda P, Díaz-Casado ME, Fernández-Gil B, Barriocanal-Casado E, Escames G, López L, Acuña-Castroviejo D (2017) Mitochondrial impairment and melatonin protection in parkinsonian mice do not depend of inducible or neuronal nitric oxide synthases. PLOS ONE 12:e0183090.


Doerrier 2020 PaduaMuscleDays Poster2020Doerrier C, Gama-Perez P, Chabi B, Ost M, Distefano G, Pesta D, Dahdah N, Lemieux H, Holody C, Carpenter RG, Tepp K, Puurand M, Kaambre T, Dubouchaud H, Cortade F, Calabria E, Casado M, Fernandez-Ortiz M, Acuna-Castroviejo D, Villena JA, Grefte S, Keijer J, O'Brien K, Sowton A, Murray AJ, Campbell MD, Marcinek DJ, Nollet E, Wuest R, Dayanidhi S, Soendergaard SD, Chroeis KM, Gonzalez-Franquesa A, Goodpaster BH, Coen PM, Larsen S, Gnaiger E, Garcia-Roves PM (2020) Commitment to reproducibility in mitochondrial respiration studies with permeabilized muscle fibers. PaduaMuscleDays.
Garcia-Roves 2019 MiPschool Coimbra2019
Pablo Garcia-Roves
Generating reference values on mitochondrial respiration in permeabilized muscle fibers.
Chabi 2019 MiP20192019
Béatrice Chabi
Generating reference values on mitochondrial respiration in permeabilized muscle fibers.
Acuna-Castroviejo 2018 MiP20182018
Dario Acuna-Castroviejo
Mitochondria, melatonin, and neuroinflammation in Parkinson's disease. Acuna-Castroviejo_Presentation
Garcia-Roves 2018 MiP2018b2018
Pablo Garcia-Roves
Generating mitochondrial respirometry reference values from permeabilized mouse soleus muscle fibers. Working Group 2 report. Garcia-Roves_Presentation
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