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Difference between revisions of "Flux control ratio"

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For a given protocol or set of respiratory protocols, flux control ratios provide a fingerprint of coupling and substrate control independent of (i) mt-content in cells or tissues, (ii) purification in preparations of isolated mitochondria, and (iii) assay conditions for determination of tissue mass or mt-markers external to a respiratory protocol (CS, protein, stereology, etc.). ''FCR'' obtained from a single respirometric incubation (sequential protocol; [[SUIT|SUIT protocol]]) provide an internal normalization, expressing respiratory control independent of mitochondrial content and thus independent of a marker for mitochondrial amount. ''FCR'' obtained from separate (parallel) protocols depend on equal distribution of subsamples obtained from a homogenous mt-preparation or determination of a common [[mitochondrial marker]].
For a given protocol or set of respiratory protocols, flux control ratios provide a fingerprint of coupling and substrate control independent of (i) mt-content in cells or tissues, (ii) purification in preparations of isolated mitochondria, and (iii) assay conditions for determination of tissue mass or mt-markers external to a respiratory protocol (CS, protein, stereology, etc.). ''FCR'' obtained from a single respirometric incubation (sequential protocol; [[SUIT|SUIT protocol]]) provide an internal normalization, expressing respiratory control independent of mitochondrial content and thus independent of a marker for mitochondrial amount. ''FCR'' obtained from separate (parallel) protocols depend on equal distribution of subsamples obtained from a homogenous mt-preparation or determination of a common [[mitochondrial marker]].
|info=[[Gnaiger 2014 MitoPathways]], [[Gnaiger 2009 Int J Biochem Cell Biol]], [[Pesta 2012 Methods Mol Biol]], [[MiPNet19.18E O2 flux analysis]]
|info=[[Gnaiger 2014 MitoPathways]], [[Gnaiger 2009 Int J Biochem Cell Biol]], [[Pesta 2012 Methods Mol Biol]], [[MiPNet19.18E O2-flux analysis]]
}}
}}
{{MitoPedia concepts
{{MitoPedia concepts

Revision as of 11:41, 14 August 2016


high-resolution terminology - matching measurements at high-resolution


Flux control ratio

Description

Flux control ratios (FCR), are ratios of oxygen flux in different respiratory control states, normalized for maximum flux in a common reference state, to obtain theoretical lower and upper limits of 0.0 and 1.0 (0% and 100%).

For a given protocol or set of respiratory protocols, flux control ratios provide a fingerprint of coupling and substrate control independent of (i) mt-content in cells or tissues, (ii) purification in preparations of isolated mitochondria, and (iii) assay conditions for determination of tissue mass or mt-markers external to a respiratory protocol (CS, protein, stereology, etc.). FCR obtained from a single respirometric incubation (sequential protocol; SUIT protocol) provide an internal normalization, expressing respiratory control independent of mitochondrial content and thus independent of a marker for mitochondrial amount. FCR obtained from separate (parallel) protocols depend on equal distribution of subsamples obtained from a homogenous mt-preparation or determination of a common mitochondrial marker.

Abbreviation: FCR

Reference: Gnaiger 2014 MitoPathways, Gnaiger 2009 Int J Biochem Cell Biol, Pesta 2012 Methods Mol Biol, MiPNet19.18E O2-flux analysis


MitoPedia concepts: Respiratory control ratio, SUIT concept 


MitoPedia methods: Respirometry 


MitoPedia O2k and high-resolution respirometry: DatLab 

Flux control factor: normalization of mitochondrial respiration

Β» More details: Flux control factor

DatLab

The entire plot of oxygen flux can be transformed to a FCR. Click on 'Slope' in the pull-down menu. - MiPNet19.18E O2 flux analysis.