Difference between revisions of "Flux control ratio"
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|description='''Flux control ratios''' (''FCR''), are ratios of oxygen flux in different respiratory control states, normalized for maximum flux in a common reference state, to obtain theoretical lower and upper limits of 0.0 and 1.0 (0% and 100%). | |description='''Flux control ratios''' (''FCR''), are ratios of oxygen flux in different respiratory control states, normalized for maximum flux in a common reference state, to obtain theoretical lower and upper limits of 0.0 and 1.0 (0% and 100%). | ||
For a given protocol or set of respiratory protocols, flux control ratios provide a fingerprint of coupling and substrate control independent of (i) mt-content | For a given protocol or set of respiratory protocols, flux control ratios provide a fingerprint of coupling and substrate control independent of (i) mt-content in cells or tissues, (ii) purification in preparations of isolated mitochondria, and (iii) assay conditions for determination of tissue mass or mt-markers external to a respiratory protocol (CS, protein, stereology, etc.). ''FCR'' obtained from a single respirometric incubation with sequential titrations (sequential protocol; [[SUIT|SUIT protocol]]) provide an internal normalization, expressing respiratory control independent of mitochondrial content and thus independent of a marker for mitochondrial amount. ''FCR'' obtained from separate (parallel) protocols depend on equal distribution of subsamples obtained from a homogenous mt-preparation or determination of a common [[mitochondrial marker]]. | ||
|info=[ | |info=[[Gnaiger 2014 MitoPathways]], [[Gnaiger 2009 Int J Biochem Cell Biol]], [[Doerrier 2018 Methods Mol Biol]] | ||
| | }} | ||
== Flux control factor: normalization of mitochondrial respiration == | |||
::::» ''More details:'' [[Flux control factor]] | |||
== DatLab == | |||
=== Unknown sample concentration and normalization per unit sample [x] === | |||
:::* In the DatLab 7.4 Excel template for oxygen flux analysis (O2 analysis template DL7.4): | |||
:::: If the sample concentration is not yet known, the box ‘Known sample concentration’ can be unchecked, and the concentration will be considered by default as 1, with units [x·mL<sup>-1</sup>]. In this way, flux can be normalized and ''FCR''s can be obtained even if the sample concentration is unknown. | |||
::::» ''Read also:'' [[Extensive quantity]]; [[BEC 2020.1 doi10.26124bec2020-0001.v1|BEC 2020.1]] | |||
::::» ''More details:'' [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4]] | |||
=== ''FCR'' in DatLab plot === | |||
:::* The entire plot of oxygen flux can be converted to a ''FCR''. Click on 'Flux/Slope' in the DatLab pull-down menu. Select chamber A or B 'O2 slope'. Select 'Flux control ratio, FCR' and select the mark that corresponds to the reference state. Change the scale under 'Graph/Scaling' (or press F6). | |||
::::» ''More details:'' [[Flux_/_Slope#Slope_configuration_menu|Flux/Slope in DatLab]] | |||
== References == | |||
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{{Template:Keywords: Normalization}} | |||
{{MitoPedia concepts | |||
|mitopedia concept=Respiratory control ratio, SUIT concept | |||
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{{MitoPedia methods | {{MitoPedia methods | ||
|mitopedia method=Respirometry | |mitopedia method=Respirometry | ||
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{{MitoPedia | {{MitoPedia O2k and high-resolution respirometry | ||
|mitopedia | |mitopedia O2k and high-resolution respirometry=DatLab | ||
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Revision as of 16:19, 15 October 2020
Description
Flux control ratios (FCR), are ratios of oxygen flux in different respiratory control states, normalized for maximum flux in a common reference state, to obtain theoretical lower and upper limits of 0.0 and 1.0 (0% and 100%).
For a given protocol or set of respiratory protocols, flux control ratios provide a fingerprint of coupling and substrate control independent of (i) mt-content in cells or tissues, (ii) purification in preparations of isolated mitochondria, and (iii) assay conditions for determination of tissue mass or mt-markers external to a respiratory protocol (CS, protein, stereology, etc.). FCR obtained from a single respirometric incubation with sequential titrations (sequential protocol; SUIT protocol) provide an internal normalization, expressing respiratory control independent of mitochondrial content and thus independent of a marker for mitochondrial amount. FCR obtained from separate (parallel) protocols depend on equal distribution of subsamples obtained from a homogenous mt-preparation or determination of a common mitochondrial marker.
Abbreviation: FCR
Reference: Gnaiger 2014 MitoPathways, Gnaiger 2009 Int J Biochem Cell Biol, Doerrier 2018 Methods Mol Biol
Flux control factor: normalization of mitochondrial respiration
- » More details: Flux control factor
DatLab
Unknown sample concentration and normalization per unit sample [x]
- In the DatLab 7.4 Excel template for oxygen flux analysis (O2 analysis template DL7.4):
- If the sample concentration is not yet known, the box ‘Known sample concentration’ can be unchecked, and the concentration will be considered by default as 1, with units [x·mL-1]. In this way, flux can be normalized and FCRs can be obtained even if the sample concentration is unknown.
- » Read also: Extensive quantity; BEC 2020.1
- » More details: MiPNet24.06 Oxygen flux analysis - DatLab 7.4
FCR in DatLab plot
- The entire plot of oxygen flux can be converted to a FCR. Click on 'Flux/Slope' in the DatLab pull-down menu. Select chamber A or B 'O2 slope'. Select 'Flux control ratio, FCR' and select the mark that corresponds to the reference state. Change the scale under 'Graph/Scaling' (or press F6).
- » More details: Flux/Slope in DatLab
References
Bioblast link | Reference | Year |
---|---|---|
Doerrier 2018 Methods Mol Biol | Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. https://doi.org/10.1007/978-1-4939-7831-1_3 | 2018 |
Gnaiger 2009 Int J Biochem Cell Biol | Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. https://doi.org/10.1016/j.biocel.2009.03.013 | 2009 |
Gnaiger 2020 BEC MitoPathways | Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002 | 2020 |
BEC 2020.1 doi10.26124bec2020-0001.v1 | Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1 | 2020 |
- Bioblast links: Normalization - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Quantities for normalization
- » Count in contrast to Number
- » Mitochondrial marker
- » O2k-Protocols: mitochondrial and marker-enzymes
- » Citrate synthase activity
- Quantities for normalization
- General
- Related keyword lists
MitoPedia concepts:
Respiratory control ratio,
SUIT concept
MitoPedia methods:
Respirometry
MitoPedia O2k and high-resolution respirometry:
DatLab