Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "Flux control ratio"

From Bioblast
Line 15: Line 15:
|type=Respiration
|type=Respiration
}}
}}
* Compare [[flux control capacity]].
* Compare [[flux control factor]].

Revision as of 12:11, 14 September 2013


high-resolution terminology - matching measurements at high-resolution


Flux control ratio

Description

Flux control ratios (FCR), are ratios of oxygen flux in different respiratory control states, normalized for maximum flux in a common reference state, to obtain theoretical lower and upper limits of 0.0 and 1.0 (0% and 100%).

For a given protocol or set of respiratory protocols, flux control ratios provide a fingerprint of coupling and substrate control independent of (i) mt-content of cells or tissues, (ii) purification in preparations of isolated mitochondria, and (iii) assay conditions for determination of tissue mass or mt-markers external to a respiratory protocol (CS, protein, stereology, etc.). FCR obtained from a single respirometric incubation (sequential protocol; SUIT protocol) provide an internal normalization, expressing respiratory control independent of mitochondrial content and thus independent of a marker for mitochondrial amount. FCR obtained from separate (parallel) protocols depend on equal distribution of subsamples obtained from a homogenous mt-preparation or determination of a common mitochondrial marker.

Abbreviation: FCR

Reference: Gnaiger 2012 MitoPathways, Gnaiger 2009 Int J Biochem Cell Biol, Pesta 2012 Methods Mol Biol


MitoPedia methods: Respirometry 


MitoPedia topics: "Respiratory control ratio" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property. Respiratory control ratio"Respiratory control ratio" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.