R-L net ROUTINE capacity

high-resolution terminology - matching measurements at high-resolution

R-L net ROUTINE capacity

Description

The free ROUTINE activity, ≈R, is ROUTINE respiration corrected for LEAK respiration, ≈R = R-L. ≈R is the respiratory activity available for phosphorylation of ADP to ATP. Oxygen consumption in the ROUTINE state of respiration measured in intact cells, therefore, is partitioned into the free ROUTINE activity, ≈R, strictly coupled to phosphorylation, ~P, and nonphosphorylating LEAK respiration, L_R, compensating for proton leaks, slip and cation cycling: R = ≈R+L_R. It is frequently assumed that LEAK respiration, L, as measured in the LEAK state, overestimates the LEAK component of respiration, L_R, as measured in the ROUTINE state, particularly if the protonmotive force is not adjusted to equivalent levels in L and L_R. However, if the LEAK component increases with enzyme turnover during R, the low enzyme turnover during L may counteract the effect of the higher Δp_mt.

Abbreviation: ≈R

Reference: Gnaiger 2014 MitoPathways

MitoPedia concepts: Respiratory state, Recommended 

MitoPedia topics: EAGLE 

Communicated by Gnaiger E 2014-09-21, edited 2015-01-19.

The Blue Book 2014: Fig. 2.4.

Compare

• Free OXPHOS capacity (mt-preparations)
• Free ET capacity