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Difference between revisions of "R-L net ROUTINE capacity"

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{{MitoPedia
{{MitoPedia
|abbr=''β‰ˆR''
|abbr=''β‰ˆR''
|description=The '''free ROUTINE activity''', ''β‰ˆR'', is [[ROUTINE respiration]] corrected for [[LEAK respiration]], ''β‰ˆR'' = ''R-L''. ''β‰ˆR'' is the respiratory activity available for phosphorylation of ADP to ATP. Oxygen consumption in the ROUTINE state of respiration measured in intact cells, therefore, is partitioned into the free ROUTINE activity, ''β‰ˆR'', strictly coupled to phosphorylation, ''~P'', and nonphosphorylating LEAK respiration, ''L<sub>R</sub>'', compensating for proton leaks, slip and cation cycling: ''R'' = ''β‰ˆR''+''L<sub>R</sub>''. It is frequently assumed that [[LEAK respiration]], ''L'', as measured in the LEAK state, overestimates the LEAK component of respiration, ''L<sub>R</sub>'', as measured in the ROUTINE state, particularly if the protonmotive force is not adjusted to equivalent levels in ''L'' and ''L<sub>R</sub>''. However, if the LEAK component increases with enzyme turnover during ''R'', the low enzyme turnover during ''L'' may counteract the effect of the higher Ξ”''p''<sub>mt</sub>.
|description=[[Image:R-L.jpg|50 px|link=Free ROUTINE activity|Free ROUTINE activity]]
The '''free ROUTINE activity''', ''β‰ˆR'', is [[ROUTINE respiration]] corrected for [[LEAK respiration]], ''β‰ˆR'' = ''R-L''. ''β‰ˆR'' is the respiratory activity available for phosphorylation of ADP to ATP. Oxygen consumption in the ROUTINE state of respiration measured in intact cells, therefore, is partitioned into the free ROUTINE activity, ''β‰ˆR'', strictly coupled to phosphorylation, ''~P'', and nonphosphorylating LEAK respiration, ''L<sub>R</sub>'', compensating for proton leaks, slip and cation cycling: ''R'' = ''β‰ˆR''+''L<sub>R</sub>''. It is frequently assumed that [[LEAK respiration]], ''L'', as measured in the LEAK state, overestimates the LEAK component of respiration, ''L<sub>R</sub>'', as measured in the ROUTINE state, particularly if the protonmotive force is not adjusted to equivalent levels in ''L'' and ''L<sub>R</sub>''. However, if the LEAK component increases with enzyme turnover during ''R'', the low enzyme turnover during ''L'' may counteract the effect of the higher Ξ”''p''<sub>mt</sub>.
|info=[[Gnaiger 2014 MitoPathways]]
|info=[[Gnaiger 2014 MitoPathways]]
}}
}}
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|mitopedia topic=Respiratory state
|mitopedia topic=Respiratory state
}}
}}
[[File:EPL-free and excess.jpg|right|240px|thumb|Blue Book 2014: Fig. 2.4.]]
[[File:EPL-free and excess.jpg|right|240px|thumb|[[Gnaiger 2014 MitoPathways |The Blue Book 2014]]: Fig. 2.4.]]
== Compare ==
== Compare ==
* [[Free OXPHOS capacity]] (mt-preparations)
* [[Free OXPHOS capacity]] (mt-preparations)
* [[Free ETS capacity]]
* [[Free ETS capacity]]

Revision as of 11:30, 18 January 2015


high-resolution terminology - matching measurements at high-resolution


R-L net ROUTINE capacity

Description

Free ROUTINE activity The free ROUTINE activity, β‰ˆR, is ROUTINE respiration corrected for LEAK respiration, β‰ˆR = R-L. β‰ˆR is the respiratory activity available for phosphorylation of ADP to ATP. Oxygen consumption in the ROUTINE state of respiration measured in intact cells, therefore, is partitioned into the free ROUTINE activity, β‰ˆR, strictly coupled to phosphorylation, ~P, and nonphosphorylating LEAK respiration, LR, compensating for proton leaks, slip and cation cycling: R = β‰ˆR+LR. It is frequently assumed that LEAK respiration, L, as measured in the LEAK state, overestimates the LEAK component of respiration, LR, as measured in the ROUTINE state, particularly if the protonmotive force is not adjusted to equivalent levels in L and LR. However, if the LEAK component increases with enzyme turnover during R, the low enzyme turnover during L may counteract the effect of the higher Ξ”pmt.

Abbreviation: β‰ˆR

Reference: Gnaiger 2014 MitoPathways


MitoPedia methods: Respirometry 


MitoPedia topics: "Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property. Respiratory state"Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property. 

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