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Friederich-Persson 2018 Acta Physiol (Oxf)

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Friederich-Persson M, Persson P, Hansell P, Palm F (2018) Deletion of Uncoupling Protein-2 reduces renal mitochondrial leak respiration, intrarenal hypoxia and proteinuria in a mouse model of type 1 diabetes. Acta Physiol (Oxf) 223:e13058.

Β» PMID: 29480974

Friederich-Persson M, Persson P, Hansell P, Palm F (2018) Acta Physiol (Oxf)

Abstract: Uncoupling protein-2 (UCP-2) can induce mitochondrial uncoupling in the diabetic kidney. Although mitochondrial uncoupling reduces oxidative stress originating from the mitochondria and can be regarded as a protective mechanism, the increased oxygen consumption occurring secondarily to increased mitochondria uncoupling, that is leak respiration, may contribute to kidney tissue hypoxia. Using UCP-2-/- mice, we tested the hypothesis that UCP-2-mediated leak respiration is important for the development of diabetes-induced intrarenal hypoxia and proteinuria.

Kidney function, in vivo oxygen metabolism, urinary protein leakage and mitochondrial function were determined in wild-type and UCP-2-/- mice during normoglycaemia and 2 weeks after diabetes induction.

Diabetic wild-type mice displayed mitochondrial leak respiration, pronounced intrarenal hypoxia, proteinuria and increased urinary KIM-1 excretion. However, diabetic UCP-2-/- mice did not develop increased mitochondrial leak respiration and presented with normal intrarenal oxygen levels, urinary protein and KIM-1 excretion.

Although functioning as an antioxidant system, mitochondria uncoupling is always in co-occurrence with increased oxygen consumption, that is leak respiration; a potentially detrimental side effect as it can result in kidney tissue hypoxia; an acknowledged unifying pathway to nephropathy. Indeed, this study demonstrates a novel mechanism in which UCP-2-mediated mitochondrial leak respiration is necessary for the development of diabetes-induced intrarenal tissue hypoxia and proteinuria.

Β© 2018 Scandinavian Physiological Society. Published by John Wiley & Sons Ltd. β€’ Keywords: Diabetic nephropathy, Kidney, Kidney injury molecule-1, Mitochondria, Oxygen consumption β€’ Bioblast editor: Kandolf G β€’ O2k-Network Lab: SE Uppsala Liss P

Labels: MiParea: Respiration  Pathology: Diabetes  Stress:Hypoxia  Organism: Mouse  Tissue;cell: Kidney  Preparation: Isolated mitochondria  Enzyme: Uncoupling protein 

Coupling state: LEAK, OXPHOS  Pathway:HRR: Oxygraph-2k 

Labels, 2018-04