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Difference between revisions of "GM-pathway control state"

From Bioblast
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|description=[[File:GM.jpg|left|200px|GM]] '''GM''': [[Glutamate]] & [[Malate]].
|description=[[File:GM.jpg|left|200px|GM]] '''GM''': [[Glutamate]] & [[Malate]].


'''MitoPathway control state:''' [[N|NADH Electron transfer-pathway state]]
'''MitoPathway control state:''' [[N|NADH electron transfer-pathway state]]


The '''GM-pathway control state''' (glutamate-malate pathway control state) is established when glutamate&malate are added to isolated mitochondria, permeabilized cells and other mitochondrial preparations. Glutamate and transaminase are responsible for the metabolism of [[oxaloacetate]], comparable to the metabolism with acetyl-CoA and citrate synthase.
The '''GM-pathway control state''' (glutamate-malate pathway control state) is established when glutamate&malate are added to isolated mitochondria, permeabilized cells and other mitochondrial preparations. Glutamate and transaminase are responsible for the metabolism of [[oxaloacetate]], comparable to the metabolism with acetyl-CoA and citrate synthase.
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::: ''More details'' Β 
::: ''More details'' Β 
::::Β» [[NADH Electron transfer-pathway state]]
::::Β» [[NADH electron transfer-pathway state]]





Revision as of 13:34, 26 August 2020


high-resolution terminology - matching measurements at high-resolution


GM-pathway control state

Description

GM

GM: Glutamate & Malate.

MitoPathway control state: NADH electron transfer-pathway state

The GM-pathway control state (glutamate-malate pathway control state) is established when glutamate&malate are added to isolated mitochondria, permeabilized cells and other mitochondrial preparations. Glutamate and transaminase are responsible for the metabolism of oxaloacetate, comparable to the metabolism with acetyl-CoA and citrate synthase.

Abbreviation: GM

Reference: Gnaiger 2014 MitoPathways - Chapter 3.4


MitoPedia concepts: SUIT state 


More details
Β» NADH electron transfer-pathway state


GML

GM pathway in the LEAK state can be evaluated in the following SUIT protocols:

GMP

GM pathway in the OXPHOS state can be evaluated in the following SUIT protocols:

  • DL-Protocol for permeabilized cells (pce):

GME

GM pathway in the ET state can be evaluated in the following SUIT protocols:

  • DL-Protocol for permeabilized cells (pce):

Discussion

In human skeletal muscle mitochondria, respiration with GM in the presence of ADP (GMP) is identical or 10 % higher than with PM (PMP). These results on isolated mitochondria agree with permeabilized fibres, although there are reports on respiratory capacity for PMP being 16% to 25% higher than for GMP. In fibroblasts, GMP supports a higher respiratory flux than PMP.