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Difference between revisions of "Gain"

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== Gain: O2 channel ==
== Gain: O2 channel ==


:::: The gain of the signal of the O2 channel oxygen sensors can be adjusted in DatLab by selecting “Oxygraph” / “O2k Control” from the menue. The gain has to be adjusted to the oxygen level in your experiment, for most applications at 37°C the pre-set gain of 1 is adequate. When the gain setting is too high for your experiment the signal will reach a constant maximum value (10 V, often displayed as 9.99 V) and the flux will therefore apparently drop to zero.  
:::: For most applications between 25 and 37 °C the pre-set gain of 1 (default) is adequate and does not have to be changed.
 
:::: The gain of the signal of the O<sub>2</sub> channel can be adjusted in DatLab by selecting [Oxygraph-2k] / [O2k Control] from the menue. When the gain setting is too high for any given signal, it will reach a constant maximum value of 10 V (often displayed as 9.99 V) and the displayed slope will therefore apparently be constant at zero.  


:::: [[File:Gain setting too high.png|600px|MitoFit-QCS]]
:::: [[File:Gain setting too high.png|600px|MitoFit-QCS]]




:::: For each gain setting a separate calibration of the oxygen signal is required. Therefore, after changing the gain you should not only perform a calibration at air saturation (as always before an experiment), but also a new zero calibration, see also [[Air calibration]]. If you frequently switch between gains, the easiest way is to store zereo calibrations in different DatLab files and use the "read from file" function in the calibration window. Alternatively, values can be entered manually.
:::: For each gain setting a separate calibration of the oxygen signal is required. Therefore, after changing the gain you should not only perform a calibration at air saturation (as always before an experiment), but also a new zero calibration, see also [[Air calibration]]. If for any reasons you frequently switch between gains, the easiest way is to store the calibrations in different DatLab files and use the "read from file" function in the calibration window. Alternatively, values can be entered manually.


:::: Oxygen signals that have been obtained after proper calibration are independent of the gain setting. Therefore oxygen concentrations (and fluxes) that have been recorded at different gain settings can of course be used together.
:::: Oxygen signals that have been obtained after proper calibration are independent of the gain setting. Therefore oxygen concentrations (and fluxes) that have been recorded at different gain settings can of course be used together.

Revision as of 09:29, 5 November 2019


high-resolution terminology - matching measurements at high-resolution


Gain

Description

The gain is an amplification factor applied to an input signal to increase the output signal.


MitoPedia O2k and high-resolution respirometry: O2k hardware, DatLab 



Template NextGen-O2k.jpg


MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



Gain: O2 channel

For most applications between 25 and 37 °C the pre-set gain of 1 (default) is adequate and does not have to be changed.
The gain of the signal of the O2 channel can be adjusted in DatLab by selecting [Oxygraph-2k] / [O2k Control] from the menue. When the gain setting is too high for any given signal, it will reach a constant maximum value of 10 V (often displayed as 9.99 V) and the displayed slope will therefore apparently be constant at zero.
MitoFit-QCS


For each gain setting a separate calibration of the oxygen signal is required. Therefore, after changing the gain you should not only perform a calibration at air saturation (as always before an experiment), but also a new zero calibration, see also Air calibration. If for any reasons you frequently switch between gains, the easiest way is to store the calibrations in different DatLab files and use the "read from file" function in the calibration window. Alternatively, values can be entered manually.
Oxygen signals that have been obtained after proper calibration are independent of the gain setting. Therefore oxygen concentrations (and fluxes) that have been recorded at different gain settings can of course be used together.
See also: Oxygen signal

Gain: pX channel

Gain: NO channel