Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "Gain"

From Bioblast
(Created page with "{{MitoPedia |description=An amplification factor of input signal to increase the output signal. }} {{MitoPedia concepts}} {{MitoPedia methods}} {{MitoPedia O2k and high-resolu...")
 
(added technical support banner)
 
(10 intermediate revisions by 3 users not shown)
Line 1: Line 1:
{{MitoPedia
{{Technical support}}
|description=An amplification factor of input signal to increase the output signal.
{{MitoPedia without banner
|description=The '''gain''' is an amplification factor applied to an input signal to increase the output signal.
}}
}}
{{MitoPedia concepts}}
__TOC__
{{MitoPedia methods}}
{{Technical support integrated}}
{{MitoPedia O2k and high-resolution respirometry
 
|mitopedia O2k and high-resolution respirometry=O2k hardware, DatLab
== Gain: O2 channel of the O2k ==
}}
 
{{MitoPedia topics}}
:::: For most applications between 25 and 37 °C the pre-set gain of 1 (default) is adequate and does not have to be changed.
{{Technical support}}
 
:::: The gain of the signal of the O<sub>2</sub> channel can be adjusted in DatLab by selecting [Oroboros O2k] / [O2k Control] / Tab [Oxygen, O2] from the menu. When the gain setting is too high for any given signal, it will reach a constant maximum value of 10 V (often displayed as 9.99 V) and the displayed slope will therefore apparently be constant at zero.


== Gain: O2 channel ==
:::: [[File:Gain setting too high.png|600px|MitoFit-QCS]]


:::: The gain of the signal of the O2 channel  oxygen sensors can be adjusted in DatLab by selecting “Oxygraph” / “O2k Control” from the menue. The gain has to be adjusted to the oxygen level in your experiment, for most applications at 37°C the pre-set gain of 1 is adequate. When the gain setting is too high for your experiment the signal will reach a constant maximum value (10 V, often displayed as 9.99 V) and the flux will therefore apparently drop to zero.


For each gain setting a separate calibration of the oxygen signal is required. Therefore, after changing the gain you should not only perform a calibration at air saturation (as always before an experiment), but also a new zero calibration, see also [[Air calibration]]. If you frequently switch between gains, the easiest way is to store zereo calibrations in different DatLab files and use the "read from file" function in the calibration window. Alternatively, values can be entered manually.
:::: For each gain setting a separate calibration of the oxygen signal is required. Therefore, after changing the gain you should not only perform a calibration at air saturation (as always before an experiment) but also a new zero calibration, see [[Air calibration]]. If for any reason you frequently switch between gains, the easiest way is to store the calibrations in different DatLab files and use the "read from file" function in the calibration window. Alternatively, values can be entered manually.


Oxygen signals that have been obtained after proper calibration are independent of the gain setting. Therefore oxygen concentrations (and fluxes) that have been recorded at different gain settings can of course be used together.
:::: Oxygen signals that have been obtained after proper calibration are independent of the gain setting. Therefore oxygen concentrations (and fluxes) that have been recorded at different gain settings can of course be used together, as long as the proper calibration is applied for these files.


see also:
:::: See also »[[Oxygen signal]]
[[Oxygen signal]]  


== Gain: pX channel of the O2k ==


{{#set:Technical service=O2 sensor|Technical service=O2 signal}}
== Gain: NO channel of the O2k ==


[[Gain (pX channel)]]
{{MitoPedia O2k and high-resolution respirometry
[[Gain (NO channel)]]
|mitopedia O2k and high-resolution respirometry=O2k hardware, DatLab
}}

Latest revision as of 10:50, 25 March 2022


                  


O2k-Open Support

Gain



Template NextGen-O2k.jpg


MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



Description

The gain is an amplification factor applied to an input signal to increase the output signal.


Template NextGen-O2k.jpg


MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



Gain: O2 channel of the O2k

For most applications between 25 and 37 °C the pre-set gain of 1 (default) is adequate and does not have to be changed.
The gain of the signal of the O2 channel can be adjusted in DatLab by selecting [Oroboros O2k] / [O2k Control] / Tab [Oxygen, O2] from the menu. When the gain setting is too high for any given signal, it will reach a constant maximum value of 10 V (often displayed as 9.99 V) and the displayed slope will therefore apparently be constant at zero.
MitoFit-QCS


For each gain setting a separate calibration of the oxygen signal is required. Therefore, after changing the gain you should not only perform a calibration at air saturation (as always before an experiment) but also a new zero calibration, see Air calibration. If for any reason you frequently switch between gains, the easiest way is to store the calibrations in different DatLab files and use the "read from file" function in the calibration window. Alternatively, values can be entered manually.
Oxygen signals that have been obtained after proper calibration are independent of the gain setting. Therefore oxygen concentrations (and fluxes) that have been recorded at different gain settings can of course be used together, as long as the proper calibration is applied for these files.
See also »Oxygen signal

Gain: pX channel of the O2k

Gain: NO channel of the O2k

MitoPedia O2k and high-resolution respirometry: O2k hardware, DatLab