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Garcia-Souza 2018 Life Sciences Meeting 2018 Innsbruck AT - Revision history
2024-03-29T14:29:01Z
Revision history for this page on the wiki
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https://wiki.oroboros.at/index.php?title=Garcia-Souza_2018_Life_Sciences_Meeting_2018_Innsbruck_AT&diff=223759&oldid=prev
Gnaiger Erich at 17:10, 10 January 2022
2022-01-10T17:10:34Z
<p></p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 17:10, 10 January 2022</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The respirometric CCVP viability test was equally applied to platelets. Additionally, the CCVP protocol yields a measure of the integrity of the mtOM and CIV activity. Taken together, our results suggest that the CCVP offers a standard operating procedure for mitochondrial respiratory studies in blood cells.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The respirometric CCVP viability test was equally applied to platelets. Additionally, the CCVP protocol yields a measure of the integrity of the mtOM and CIV activity. Taken together, our results suggest that the CCVP offers a standard operating procedure for mitochondrial respiratory studies in blood cells.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|editor=[[Garcia-Souza LF]],</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|editor=[[Garcia-Souza LF]],</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=<del style="font-weight: bold; text-decoration: none;">AT Innsbruck Gnaiger E, </del>AT Innsbruck Oroboros</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck Oroboros</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Affiliations ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Affiliations ==</div></td></tr>
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Gnaiger Erich
https://wiki.oroboros.at/index.php?title=Garcia-Souza_2018_Life_Sciences_Meeting_2018_Innsbruck_AT&diff=175395&oldid=prev
Gnaiger Erich at 22:08, 17 March 2019
2019-03-17T22:08:44Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 22:08, 17 March 2019</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|abstract=Mitochondrial (mt) respiration of blood cells represents a powerful diagnostic model for evaluation of mt-function in health and disease. However, standard operating procedures have to be established. We developed a substrate-uncoupler-inhibitor titration protocol to measure respiratory control in intact cells and cell viability in an integrated assay: the coupling control and cell viability protocol (CCVP) using mitochondrial respiration medium MiR06 and cell culture media RPMI and M199.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|abstract=Mitochondrial (mt) respiration of blood cells represents a powerful diagnostic model for evaluation of mt-function in health and disease. However, standard operating procedures have to be established. We developed a substrate-uncoupler-inhibitor titration protocol to measure respiratory control in intact cells and cell viability in an integrated assay: the coupling control and cell viability protocol (CCVP) using mitochondrial respiration medium MiR06 and cell culture media RPMI and M199.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Respiration was determined in isolated peripheral blood mononuclear cells (PBMC) and platelets from healthy volunteers by <del style="font-weight: bold; text-decoration: none;">High</del>-<del style="font-weight: bold; text-decoration: none;">Resolution FluoRespirometry</del>. ROUTINE respiration of intact cells (ce) was measured before and after addition of pyruvate. Oligomycin (0.05 – 2.5 µM, inhibitor of F-ATPase) was added to obtain LEAK respiration. Electron transfer-capacity (''E'') was stimulated by titration of CCCP (uncoupler), followed by glucose addition (Crabtree effect). Malate was added to stimulate respiration of cells without intact plasma membrane (dead cells, dce). Rotenone inhibits intact cells to the level of residual oxygen consumption (''Rox''), after which succinate stimulates respiration of dce. The entire cell population was permeabilized by titration of digitonin, providing a reference level of succinate respiration (permeabilized cells, pce). Cytochrome ''c'' was added to test the intactness of the mt-outer membrane (mtOM). CIII was inhibited by antimycin A, to measure CIV activity by addition of ascorbate and TMPD and later inhibition by azide.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Respiration was determined in isolated peripheral blood mononuclear cells (PBMC) and platelets from healthy volunteers by <ins style="font-weight: bold; text-decoration: none;">high</ins>-<ins style="font-weight: bold; text-decoration: none;">resolution respirometry</ins>. ROUTINE respiration of intact cells (ce) was measured before and after addition of pyruvate. Oligomycin (0.05 – 2.5 µM, inhibitor of F-ATPase) was added to obtain LEAK respiration. Electron transfer-capacity (''E'') was stimulated by titration of CCCP (uncoupler), followed by glucose addition (Crabtree effect). Malate was added to stimulate respiration of cells without intact plasma membrane (dead cells, dce). Rotenone inhibits intact cells to the level of residual oxygen consumption (''Rox''), after which succinate stimulates respiration of dce. The entire cell population was permeabilized by titration of digitonin, providing a reference level of succinate respiration (permeabilized cells, pce). Cytochrome ''c'' was added to test the intactness of the mt-outer membrane (mtOM). CIII was inhibited by antimycin A, to measure CIV activity by addition of ascorbate and TMPD and later inhibition by azide.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>ROUTINE respiration in MiR06 was higher in PBMCs but lower in platelets compared to cell culture media. Pyruvate stimulated respiration (''p''<0.02) in both cell types and respiration was higher in MiR06 than in cell culture media containing glucose after uncoupling. 0.01 µM oligomycin inhibited LEAK respiration as much as 2.5 µM, without or only slight inhibitory effect on ''E'' in PBMCs and platelets, respectively. In PBMCs, the CCVP viability test, 1-(dce-Rox)/(pce-Rox), resulted in 94% viability which correlated with 96% obtained with acridine orange/propidium iodide staining.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>ROUTINE respiration in MiR06 was higher in PBMCs but lower in platelets compared to cell culture media. Pyruvate stimulated respiration (''p''<0.02) in both cell types and respiration was higher in MiR06 than in cell culture media containing glucose after uncoupling. 0.01 µM oligomycin inhibited LEAK respiration as much as 2.5 µM, without or only slight inhibitory effect on ''E'' in PBMCs and platelets, respectively. In PBMCs, the CCVP viability test, 1-(dce-Rox)/(pce-Rox), resulted in 94% viability which correlated with 96% obtained with acridine orange/propidium iodide staining.</div></td></tr>
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Gnaiger Erich
https://wiki.oroboros.at/index.php?title=Garcia-Souza_2018_Life_Sciences_Meeting_2018_Innsbruck_AT&diff=165231&oldid=prev
Gnaiger Erich at 10:08, 3 November 2018
2018-11-03T10:08:36Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 10:08, 3 November 2018</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck Gnaiger E, AT Innsbruck Oroboros</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck Gnaiger E, AT Innsbruck Oroboros</div></td></tr>
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<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">== Affiliations ==</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">:::: Garcia-Souza LF(1,2), Cizmarova B(1,3), Volani C(4), Sumbalova Z(1), Doerrier C(5), Gnaiger E(1,5)</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">::::# Daniel Swarovski Research Lab, Dept Visceral, Transplant Thoracic Surgery, Medical Univ Innsbruck, Austria</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">::::# Inst Sport Science, Univ Innsbruck, Austria</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">::::# Dept Medical Clinical Biochem, Fac Medicine, Pavol Jozef Šafárik Univ Košice, Slovakia</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">::::# Dept Internal Medicine II, Medical Univ Innsbruck, Austria</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">::::# Oroboros Instruments, Innsbruck, Austria. - luiz.garcia@student.i-med.ac.at</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"></ins></div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|area=Respiration, Instruments;methods</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|area=Respiration, Instruments;methods</div></td></tr>
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<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|additional=MitoEAGLE<del style="font-weight: bold; text-decoration: none;">,</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|additional=MitoEAGLE</div></td></tr>
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<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">== Affiliations ==</del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">:::: Garcia-Souza LF(1,2), Cizmarova B(1,3), Volani C(4), Sumbalova Z(1), Doerrier C(5), Gnaiger E(1,5)</del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">::::# Daniel Swarovski Research Lab, Dept Visceral, Transplant Thoracic Surgery, Medical Univ Innsbruck, Austria</del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">::::# Inst Sport Science, Univ Innsbruck, Austria</del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">::::# Dept Medical Clinical Biochem, Fac Medicine, Pavol Jozef Šafárik Univ Košice, Slovakia</del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">::::# Dept Internal Medicine II, Medical Univ Innsbruck, Austria</del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">::::# Oroboros Instruments, Innsbruck, Austria. - luiz.garcia@student.i-med.ac.at</del></div></td><td colspan="2"></td></tr>
</table>
Gnaiger Erich
https://wiki.oroboros.at/index.php?title=Garcia-Souza_2018_Life_Sciences_Meeting_2018_Innsbruck_AT&diff=165230&oldid=prev
Gnaiger Erich at 10:07, 3 November 2018
2018-11-03T10:07:46Z
<p></p>
<table style="background-color: #fff; color: #202122;" data-mw="interface">
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 10:07, 3 November 2018</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l6">Line 6:</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|abstract=Mitochondrial (mt) respiration of blood cells represents a powerful diagnostic model for evaluation of mt-function in health and disease. However, standard operating procedures have to be established. We developed a substrate-uncoupler-inhibitor titration protocol to measure respiratory control in intact cells and cell viability in an integrated assay: the coupling control and cell viability protocol (CCVP) using mitochondrial respiration medium MiR06 and cell culture media RPMI and M199.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|abstract=Mitochondrial (mt) respiration of blood cells represents a powerful diagnostic model for evaluation of mt-function in health and disease. However, standard operating procedures have to be established. We developed a substrate-uncoupler-inhibitor titration protocol to measure respiratory control in intact cells and cell viability in an integrated assay: the coupling control and cell viability protocol (CCVP) using mitochondrial respiration medium MiR06 and cell culture media RPMI and M199.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Respiration was determined in isolated peripheral blood mononuclear cells (PBMC) and platelets from healthy volunteers by High-Resolution FluoRespirometry. ROUTINE respiration of intact cells (ce) was measured before and after addition of pyruvate. Oligomycin (0.05 – 2.5 µM, inhibitor of F-ATPase) was added to obtain LEAK respiration. Electron transfer-capacity (E) was stimulated by titration of CCCP (uncoupler), followed by glucose addition (Crabtree effect). Malate was added to stimulate respiration of cells without intact plasma membrane (dead cells, dce). Rotenone inhibits intact cells to the level of residual oxygen consumption (Rox), after which succinate stimulates respiration of dce. The entire cell population was permeabilized by titration of digitonin, providing a reference level of succinate respiration (permeabilized cells, pce). Cytochrome c was added to test the intactness of the mt-outer membrane (mtOM). CIII was inhibited by antimycin A, to measure CIV activity by addition of ascorbate and TMPD and later inhibition by azide.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Respiration was determined in isolated peripheral blood mononuclear cells (PBMC) and platelets from healthy volunteers by High-Resolution FluoRespirometry. ROUTINE respiration of intact cells (ce) was measured before and after addition of pyruvate. Oligomycin (0.05 – 2.5 µM, inhibitor of F-ATPase) was added to obtain LEAK respiration. Electron transfer-capacity (<ins style="font-weight: bold; text-decoration: none;">''</ins>E<ins style="font-weight: bold; text-decoration: none;">''</ins>) was stimulated by titration of CCCP (uncoupler), followed by glucose addition (Crabtree effect). Malate was added to stimulate respiration of cells without intact plasma membrane (dead cells, dce). Rotenone inhibits intact cells to the level of residual oxygen consumption (<ins style="font-weight: bold; text-decoration: none;">''</ins>Rox<ins style="font-weight: bold; text-decoration: none;">''</ins>), after which succinate stimulates respiration of dce. The entire cell population was permeabilized by titration of digitonin, providing a reference level of succinate respiration (permeabilized cells, pce). Cytochrome <ins style="font-weight: bold; text-decoration: none;">''</ins>c<ins style="font-weight: bold; text-decoration: none;">'' </ins>was added to test the intactness of the mt-outer membrane (mtOM). CIII was inhibited by antimycin A, to measure CIV activity by addition of ascorbate and TMPD and later inhibition by azide.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>ROUTINE respiration in MiR06 was higher in PBMCs but lower in platelets compared to cell culture media. Pyruvate stimulated respiration (p<0.02) in both cell types and respiration was higher in MiR06 than in cell culture media containing glucose after uncoupling. 0.01 µM oligomycin inhibited LEAK respiration as much as 2.5 µM, without or only slight inhibitory effect on E in PBMCs and platelets, respectively. In PBMCs, the CCVP viability test, 1-(dce-Rox)/(pce-Rox), resulted in 94% viability which correlated with 96% obtained with acridine orange/propidium iodide staining.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>ROUTINE respiration in MiR06 was higher in PBMCs but lower in platelets compared to cell culture media. Pyruvate stimulated respiration (<ins style="font-weight: bold; text-decoration: none;">''</ins>p<ins style="font-weight: bold; text-decoration: none;">''</ins><0.02) in both cell types and respiration was higher in MiR06 than in cell culture media containing glucose after uncoupling. 0.01 µM oligomycin inhibited LEAK respiration as much as 2.5 µM, without or only slight inhibitory effect on <ins style="font-weight: bold; text-decoration: none;">''</ins>E<ins style="font-weight: bold; text-decoration: none;">'' </ins>in PBMCs and platelets, respectively. In PBMCs, the CCVP viability test, 1-(dce-Rox)/(pce-Rox), resulted in 94% viability which correlated with 96% obtained with acridine orange/propidium iodide staining.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The respirometric CCVP viability test was equally applied to platelets. Additionally, the CCVP protocol yields a measure of the integrity of the mtOM and CIV activity. Taken together, our results suggest that the CCVP offers a standard operating procedure for mitochondrial respiratory studies in blood cells.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The respirometric CCVP viability test was equally applied to platelets. Additionally, the CCVP protocol yields a measure of the integrity of the mtOM and CIV activity. Taken together, our results suggest that the CCVP offers a standard operating procedure for mitochondrial respiratory studies in blood cells.</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|event=Poster</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|event=Poster</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Affiliations ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Affiliations ==</div></td></tr>
</table>
Gnaiger Erich
https://wiki.oroboros.at/index.php?title=Garcia-Souza_2018_Life_Sciences_Meeting_2018_Innsbruck_AT&diff=165229&oldid=prev
Gnaiger Erich at 10:04, 3 November 2018
2018-11-03T10:04:59Z
<p></p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 10:04, 3 November 2018</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Abstract</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Abstract</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|title=A respirometric cell viability test for peripheral-blood mononuclear cells and platelets.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|title=A respirometric cell viability test for peripheral-blood mononuclear cells and platelets.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|authors=Garcia-Souza LF, Cizmarova B, Volani C, Sumbalova Z, Gnaiger E</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|authors=Garcia-Souza LF, Cizmarova B, Volani C, Sumbalova Z<ins style="font-weight: bold; text-decoration: none;">, Doerrier C</ins>, Gnaiger E</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|year=2018</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|year=2018</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|event=Life Sciences Meeting 2018 Innsbruck AT</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|event=Life Sciences Meeting 2018 Innsbruck AT</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Affiliations ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Affiliations ==</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::Garcia-Souza LF(1,2), Cizmarova B(1,3), Volani C(4), Sumbalova Z(1), Gnaiger E(1,5)</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:::: Garcia-Souza LF(1,2), Cizmarova B(1,3), Volani C(4), Sumbalova Z(1<ins style="font-weight: bold; text-decoration: none;">), Doerrier C(5</ins>), Gnaiger E(1,5)</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::#Daniel Swarovski Research Lab, Dept Visceral, Transplant Thoracic Surgery, Medical Univ Innsbruck, Austria</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::# Daniel Swarovski Research Lab, Dept Visceral, Transplant Thoracic Surgery, Medical Univ Innsbruck, Austria</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::#Inst Sport Science, Univ Innsbruck, Austria</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::# Inst Sport Science, Univ Innsbruck, Austria</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::#Dept Medical Clinical Biochem, Fac Medicine, Pavol Jozef Šafárik Univ Košice, Slovakia</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::# Dept Medical Clinical Biochem, Fac Medicine, Pavol Jozef Šafárik Univ Košice, Slovakia</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::#Dept Internal Medicine II, Medical Univ Innsbruck, Austria</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::# Dept Internal Medicine II, Medical Univ Innsbruck, Austria</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::#Oroboros Instruments, Innsbruck, Austria. - luiz.garcia@student.i-med.ac.at</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::# Oroboros Instruments, Innsbruck, Austria. - luiz.garcia@student.i-med.ac.at</div></td></tr>
</table>
Gnaiger Erich
https://wiki.oroboros.at/index.php?title=Garcia-Souza_2018_Life_Sciences_Meeting_2018_Innsbruck_AT&diff=163262&oldid=prev
Beno Marija at 13:23, 1 October 2018
2018-10-01T13:23:51Z
<p></p>
<table style="background-color: #fff; color: #202122;" data-mw="interface">
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 13:23, 1 October 2018</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Abstract</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Abstract</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|title=A respirometric cell viability test for peripheral-blood mononuclear cells and platelets.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|title=A respirometric cell viability test for peripheral-blood mononuclear cells and platelets.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|authors=Garcia-Souza LF, <del style="font-weight: bold; text-decoration: none;">Velika </del>B, Volani C, Sumbalova Z, Gnaiger E</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|authors=Garcia-Souza LF, <ins style="font-weight: bold; text-decoration: none;">Cizmarova </ins>B, Volani C, Sumbalova Z, Gnaiger E</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|year=2018</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|year=2018</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|event=Life Sciences Meeting 2018 Innsbruck AT</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|event=Life Sciences Meeting 2018 Innsbruck AT</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Affiliations ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Affiliations ==</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::Garcia-Souza LF(1,2), <del style="font-weight: bold; text-decoration: none;">Velika </del>B(1,3), Volani C(4), Sumbalova Z(1), Gnaiger E(1,5)</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::Garcia-Souza LF(1,2), <ins style="font-weight: bold; text-decoration: none;">Cizmarova </ins>B(1,3), Volani C(4), Sumbalova Z(1), Gnaiger E(1,5)</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::#Daniel Swarovski Research Lab, Dept Visceral, Transplant Thoracic Surgery, Medical Univ Innsbruck, Austria</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::#Daniel Swarovski Research Lab, Dept Visceral, Transplant Thoracic Surgery, Medical Univ Innsbruck, Austria</div></td></tr>
</table>
Beno Marija
https://wiki.oroboros.at/index.php?title=Garcia-Souza_2018_Life_Sciences_Meeting_2018_Innsbruck_AT&diff=156017&oldid=prev
Garcia Luiz at 15:05, 5 April 2018
2018-04-05T15:05:31Z
<p></p>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Respiration was determined in isolated peripheral blood mononuclear cells (PBMC) and platelets from healthy volunteers by High-Resolution FluoRespirometry. ROUTINE respiration of intact cells (ce) was measured before and after addition of pyruvate. Oligomycin (0.05 – 2.5 µM, inhibitor of F-ATPase) was added to obtain LEAK respiration. Electron transfer-capacity (E) was stimulated by titration of CCCP (uncoupler), followed by glucose addition (Crabtree effect). Malate was added to stimulate respiration of cells without intact plasma membrane (dead cells, dce). Rotenone inhibits intact cells to the level of residual oxygen consumption (Rox), after which succinate stimulates respiration of dce. The entire cell population was permeabilized by titration of digitonin, providing a reference level of succinate respiration (permeabilized cells, pce). Cytochrome c was added to test the intactness of the mt-outer membrane (mtOM). CIII was inhibited by antimycin A, to measure CIV activity by addition of ascorbate and TMPD and later inhibition by azide.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Respiration was determined in isolated peripheral blood mononuclear cells (PBMC) and platelets from healthy volunteers by High-Resolution FluoRespirometry. ROUTINE respiration of intact cells (ce) was measured before and after addition of pyruvate. Oligomycin (0.05 – 2.5 µM, inhibitor of F-ATPase) was added to obtain LEAK respiration. Electron transfer-capacity (E) was stimulated by titration of CCCP (uncoupler), followed by glucose addition (Crabtree effect). Malate was added to stimulate respiration of cells without intact plasma membrane (dead cells, dce). Rotenone inhibits intact cells to the level of residual oxygen consumption (Rox), after which succinate stimulates respiration of dce. The entire cell population was permeabilized by titration of digitonin, providing a reference level of succinate respiration (permeabilized cells, pce). Cytochrome c was added to test the intactness of the mt-outer membrane (mtOM). CIII was inhibited by antimycin A, to measure CIV activity by addition of ascorbate and TMPD and later inhibition by azide.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>ROUTINE respiration in MiR06 was higher in PBMCs but lower in platelets compared to cell culture media. Pyruvate stimulated respiration (p<0.02) in both cell types and respiration was higher in MiR06 than in cell culture media containing glucose after uncoupling. 0.<del style="font-weight: bold; text-decoration: none;">1 </del>µM oligomycin inhibited LEAK respiration as much as 2.5 µM, without or only slight inhibitory effect on E in PBMCs and platelets, respectively. In PBMCs, the CCVP viability test, 1-(dce-Rox)/(pce-Rox), resulted in 94% viability which correlated with 96% obtained with acridine orange/propidium iodide staining.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>ROUTINE respiration in MiR06 was higher in PBMCs but lower in platelets compared to cell culture media. Pyruvate stimulated respiration (p<0.02) in both cell types and respiration was higher in MiR06 than in cell culture media containing glucose after uncoupling. 0.<ins style="font-weight: bold; text-decoration: none;">01 </ins>µM oligomycin inhibited LEAK respiration as much as 2.5 µM, without or only slight inhibitory effect on E in PBMCs and platelets, respectively. In PBMCs, the CCVP viability test, 1-(dce-Rox)/(pce-Rox), resulted in 94% viability which correlated with 96% obtained with acridine orange/propidium iodide staining.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The respirometric CCVP viability test was equally applied to platelets. Additionally, the CCVP protocol yields a measure of the integrity of the mtOM and CIV activity. Taken together, our results suggest that the CCVP offers a standard operating procedure for mitochondrial respiratory studies in blood cells.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The respirometric CCVP viability test was equally applied to platelets. Additionally, the CCVP protocol yields a measure of the integrity of the mtOM and CIV activity. Taken together, our results suggest that the CCVP offers a standard operating procedure for mitochondrial respiratory studies in blood cells.</div></td></tr>
</table>
Garcia Luiz
https://wiki.oroboros.at/index.php?title=Garcia-Souza_2018_Life_Sciences_Meeting_2018_Innsbruck_AT&diff=155959&oldid=prev
Kandolf Georg at 11:39, 4 April 2018
2018-04-04T11:39:31Z
<p></p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 11:39, 4 April 2018</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The respirometric CCVP viability test was equally applied to platelets. Additionally, the CCVP protocol yields a measure of the integrity of the mtOM and CIV activity. Taken together, our results suggest that the CCVP offers a standard operating procedure for mitochondrial respiratory studies in blood cells.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The respirometric CCVP viability test was equally applied to platelets. Additionally, the CCVP protocol yields a measure of the integrity of the mtOM and CIV activity. Taken together, our results suggest that the CCVP offers a standard operating procedure for mitochondrial respiratory studies in blood cells.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|editor=[[Garcia-Souza LF]],</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|editor=[[Garcia-Souza LF]],</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck Oroboros</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=<ins style="font-weight: bold; text-decoration: none;">AT Innsbruck Gnaiger E, </ins>AT Innsbruck Oroboros</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Labeling</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Labeling</div></td></tr>
</table>
Kandolf Georg
https://wiki.oroboros.at/index.php?title=Garcia-Souza_2018_Life_Sciences_Meeting_2018_Innsbruck_AT&diff=155958&oldid=prev
Kandolf Georg at 11:25, 4 April 2018
2018-04-04T11:25:42Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 11:25, 4 April 2018</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Abstract</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Abstract</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|title=A respirometric cell viability test for peripheral-blood mononuclear cells and platelets</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|title=A respirometric cell viability test for peripheral-blood mononuclear cells and platelets<ins style="font-weight: bold; text-decoration: none;">.</ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|authors=Garcia-Souza LF, Velika B, Volani C, Sumbalova Z, Gnaiger E</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|authors=Garcia-Souza LF, Velika B, Volani C, Sumbalova Z, Gnaiger E</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|year=2018</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|year=2018</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|abstract=Mitochondrial (mt) respiration of blood cells represents a powerful diagnostic model for evaluation of mt-function in health and disease. However, standard operating procedures have to be established. We developed a substrate-uncoupler-inhibitor titration protocol to measure respiratory control in intact cells and cell viability in an integrated assay: the coupling control and cell viability protocol (CCVP) using mitochondrial respiration medium MiR06 and cell culture media RPMI and M199.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|abstract=Mitochondrial (mt) respiration of blood cells represents a powerful diagnostic model for evaluation of mt-function in health and disease. However, standard operating procedures have to be established. We developed a substrate-uncoupler-inhibitor titration protocol to measure respiratory control in intact cells and cell viability in an integrated assay: the coupling control and cell viability protocol (CCVP) using mitochondrial respiration medium MiR06 and cell culture media RPMI and M199.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Respiration was determined in isolated peripheral blood mononuclear cells (PBMC) and platelets from healthy volunteers by <del style="font-weight: bold; text-decoration: none;">high</del>-<del style="font-weight: bold; text-decoration: none;">resolution respirometry</del>. ROUTINE respiration of intact cells (ce) was measured before and after addition of pyruvate. Oligomycin (0.05 – 2.5 µM, inhibitor of F-ATPase) was added to obtain LEAK respiration. Electron transfer-capacity (E) was stimulated by titration of CCCP (uncoupler), followed by glucose addition (Crabtree effect). Malate was added to stimulate respiration of cells without intact plasma membrane (dead cells, dce). Rotenone inhibits intact cells to the level of residual oxygen consumption (Rox), after which succinate stimulates respiration of dce. The entire cell population was permeabilized by titration of digitonin, providing a reference level of succinate respiration (permeabilized cells, pce). Cytochrome c was added to test the intactness of the mt-outer membrane (mtOM). CIII was inhibited by antimycin A, to measure CIV activity by addition of ascorbate and TMPD and later inhibition by azide.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Respiration was determined in isolated peripheral blood mononuclear cells (PBMC) and platelets from healthy volunteers by <ins style="font-weight: bold; text-decoration: none;">High</ins>-<ins style="font-weight: bold; text-decoration: none;">Resolution FluoRespirometry</ins>. ROUTINE respiration of intact cells (ce) was measured before and after addition of pyruvate. Oligomycin (0.05 – 2.5 µM, inhibitor of F-ATPase) was added to obtain LEAK respiration. Electron transfer-capacity (E) was stimulated by titration of CCCP (uncoupler), followed by glucose addition (Crabtree effect). Malate was added to stimulate respiration of cells without intact plasma membrane (dead cells, dce). Rotenone inhibits intact cells to the level of residual oxygen consumption (Rox), after which succinate stimulates respiration of dce. The entire cell population was permeabilized by titration of digitonin, providing a reference level of succinate respiration (permeabilized cells, pce). Cytochrome c was added to test the intactness of the mt-outer membrane (mtOM). CIII was inhibited by antimycin A, to measure CIV activity by addition of ascorbate and TMPD and later inhibition by azide.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>ROUTINE respiration in MiR06 was higher in PBMCs but lower in platelets compared to cell culture media. Pyruvate stimulated respiration (p<0.02) in both cell types and respiration was higher in MiR06 than in cell culture media containing glucose after uncoupling. 0.1 µM oligomycin inhibited LEAK respiration as much as 2.5 µM, without or only slight inhibitory effect on E in PBMCs and platelets, respectively. In PBMCs, the CCVP viability test, 1-(dce-Rox)/(pce-Rox), resulted in 94% viability which correlated with 96% obtained with acridine orange/propidium iodide staining.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>ROUTINE respiration in MiR06 was higher in PBMCs but lower in platelets compared to cell culture media. Pyruvate stimulated respiration (p<0.02) in both cell types and respiration was higher in MiR06 than in cell culture media containing glucose after uncoupling. 0.1 µM oligomycin inhibited LEAK respiration as much as 2.5 µM, without or only slight inhibitory effect on E in PBMCs and platelets, respectively. In PBMCs, the CCVP viability test, 1-(dce-Rox)/(pce-Rox), resulted in 94% viability which correlated with 96% obtained with acridine orange/propidium iodide staining.</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::#<del style="font-weight: bold; text-decoration: none;">1Daniel </del>Swarovski Research Lab, Dept Visceral, Transplant Thoracic Surgery, Medical Univ Innsbruck, Austria</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::#<ins style="font-weight: bold; text-decoration: none;">Daniel </ins>Swarovski Research Lab, Dept Visceral, Transplant Thoracic Surgery, Medical Univ Innsbruck, Austria</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::#Inst Sport Science, Univ Innsbruck, Austria</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::#Inst Sport Science, Univ Innsbruck, Austria</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::#Dept Medical Clinical Biochem, Fac Medicine, Pavol Jozef Šafárik Univ Košice, Slovakia</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::#Dept Medical Clinical Biochem, Fac Medicine, Pavol Jozef Šafárik Univ Košice, Slovakia</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::#Dept Internal Medicine II, Medical Univ Innsbruck, Austria</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::#Dept Internal Medicine II, Medical Univ Innsbruck, Austria</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::#<del style="font-weight: bold; text-decoration: none;">5Oroboros </del>Instruments, Innsbruck, Austria</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::#<ins style="font-weight: bold; text-decoration: none;">Oroboros </ins>Instruments, Innsbruck, Austria<ins style="font-weight: bold; text-decoration: none;">. - luiz.garcia@student.i-med.ac.at</ins></div></td></tr>
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Kandolf Georg
https://wiki.oroboros.at/index.php?title=Garcia-Souza_2018_Life_Sciences_Meeting_2018_Innsbruck_AT&diff=155918&oldid=prev
Garcia Luiz at 10:56, 2 April 2018
2018-04-02T10:56:48Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 10:56, 2 April 2018</td>
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Garcia Luiz