Garnier 2005 FASEB J

Garnier A, Fortin D, Zoll J, N'Guessan B, Mettauer B, Lampert E, Veksler V, Ventura-Clapier R (2005) Coordinated changes in mitochondrial function and biogenesis in healthy and diseased human skeletal muscle. FASEB J 19:43-52.

» PMID: 15629894

Garnier A, Fortin D, Zoll J, N'Guessan B, Mettauer B, Lampert E, Veksler V, Ventura-Clapier R (2005) FASEB J

Abstract: We examined the transcriptional signaling cascade involved in the changes of mitochondrial biogenesis and mitochondrial function of skeletal muscle and of the exercise capacity of humans in response to long-term physical activity and chronic heart failure (CHF). Biopsy samples of vastus lateralis muscle were obtained from 18 healthy subjects with different fitness levels (assessed by maximal oxygen uptake, VO2 peak). We compared 9 sedentary subjects with 10 CHF patients undergoing transplantation. Muscle oxidative capacity was measured in permeabilized fibers (Vmax). Transcript levels of target genes were quantified by RT-PCR. In healthy subjects, VO2 peak was linearly related to Vmax (P<0.01) and to the gene expression of mitochondrial proteins and of the coactivator PGC-1alpha and its downstream transcription factors. A coordinate increase in PGC-1alpha and mRNA levels of proteins involved in degradation, fusion, and fission of mitochondria was observed associated with calcineurin activation. Despite decreased VO2 peak, in CHF patients skeletal muscles showed preserved Vmax in accordance with preserved markers and transcription factors of mitochondrial biogenesis and dynamics, with no calcineurin activation. The results provide strong support for a central role for PGC-1alpha and calcineurin activation in mitochondrial biogenesis in healthy and diseased human skeletal muscles.

• Bioblast editor: Gnaiger E

Labels: MiParea: Respiration 

Organism: Human  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue 

Coupling state: OXPHOS  Pathway: N 

MitoEAGLE BME 

MitoEAGLE V_O2max/BME data base

• Human vastus lateralis
• 1 female & 8 males
• 52 years
• Sedentary, not engaged in leisure or professional physical activity
• h = 1.74 m
• m = 86.6 kg
• BME = 1.40
• BMI = 28.6 kg·m^-2
• V_O2max/BM = 29.6 mL·min^-1·kg^-1 (= V_O2peak/BM/0.93)
• Permeabilized muscle fibres; 22 °C; GM_P; m_d; conversions: Gnaiger 2009 Int J Biochem Cell Biol
• J_O2,P(NS) = 63.5 µmol·s^-1·kg^-1 wet muscle mass (37 °C)

• J_O2,P(GM) = 46.3 µmol·s^-1·kg^-1 wet muscle mass (37 °C)
• J_O2,P(NS) = J_O2,P(GM)/0.73
• Fiber wet mass to dry mass ratio = 3.5 (N'Guessan 2004 Mol Cell Biochem)

---