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Difference between revisions of "Gellerich 2009 PLoS One"

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|abstract=We present unexpected and novel results revealing that glutamate-dependent oxidative phosphorylation (OXPHOS) of brain mitochondria is exclusively and efficiently activated by extramitochondrial Ca<sup>2+</sup> in physiological concentration ranges (S<sub>0.5</sub>= 360 nM Ca<sup>2+</sup>). This regulation was not affected by RR, an inhibitor of the mitochondrial Ca<sup>2+</sup> uniporter. Active respiration is regulated by glutamate supply to mitochondria via aralar, a mitochondrial glutamate/aspartate carrier with regulatory Ca<sup>2+</sup>-binding sites in the mitochondrial intermembrane space providing full access to cytosolic Ca<sup>2+</sup>. At micromolar concentrations, Ca<sup>2+</sup> can also enter the intramitochondrial matrix and activate specific dehydrogenases. However, the latter mechanism is less efficient than extramitochondrial Ca<sup>2+</sup> regulation of respiration/OXPHOS via aralar. These results imply a new mode of glutamate-dependent OXPHOS regulation as a demand-driven regulation of mitochondrial function. This regulation involves the mitochondrial glutamate/aspartate carrier aralar which controls mitochondrial substrate supply according to the level of extramitochondrial Ca<sup>2+</sup>.
|abstract=We present unexpected and novel results revealing that glutamate-dependent oxidative phosphorylation (OXPHOS) of brain mitochondria is exclusively and efficiently activated by extramitochondrial Ca<sup>2+</sup> in physiological concentration ranges (S<sub>0.5</sub>= 360 nM Ca<sup>2+</sup>). This regulation was not affected by RR, an inhibitor of the mitochondrial Ca<sup>2+</sup> uniporter. Active respiration is regulated by glutamate supply to mitochondria via aralar, a mitochondrial glutamate/aspartate carrier with regulatory Ca<sup>2+</sup>-binding sites in the mitochondrial intermembrane space providing full access to cytosolic Ca<sup>2+</sup>. At micromolar concentrations, Ca<sup>2+</sup> can also enter the intramitochondrial matrix and activate specific dehydrogenases. However, the latter mechanism is less efficient than extramitochondrial Ca<sup>2+</sup> regulation of respiration/OXPHOS via aralar. These results imply a new mode of glutamate-dependent OXPHOS regulation as a demand-driven regulation of mitochondrial function. This regulation involves the mitochondrial glutamate/aspartate carrier aralar which controls mitochondrial substrate supply according to the level of extramitochondrial Ca<sup>2+</sup>.
|keywords=Glutamate/aspartate carrier aralar, Extramitochondrial Ca (2+), Brain mitochondria
|keywords=Glutamate/aspartate carrier aralar, Extramitochondrial Ca (2+), Brain mitochondria
|mipnetlab=EE Tartu Seppet EK, DE Magdeburg Gellerich FN
|mipnetlab=EE_Tartu Paju K, DE Magdeburg Gellerich FN
|discipline=Mitochondrial Physiology, Biomedicine
|discipline=Mitochondrial Physiology, Biomedicine
}}
}}
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|couplingstates=OXPHOS
|couplingstates=OXPHOS
|instruments=Oxygraph-2k
|instruments=Oxygraph-2k
|additional=Ca-list
|discipline=Mitochondrial Physiology, Biomedicine
|discipline=Mitochondrial Physiology, Biomedicine
}}
}}

Latest revision as of 08:28, 7 May 2020

Publications in the MiPMap
Gellerich FN, Gizatullina Z, Arandarcikaite O, Jerzembek D, Vielhaber S, Seppet E, Striggow F (2009) Extramitochondrial Ca2+ in the nanomolar range regulates glutamate-dependent oxidative phosphorylation on demand. PloS One 4:e8181.

Β» PMID: 20011041 Open Access

Gellerich FN, Gizatullina Z, Arandarcikaite O, Jerzembek D, Vielhaber S, Seppet E, Striggow F (2009) PLoS One

Abstract: We present unexpected and novel results revealing that glutamate-dependent oxidative phosphorylation (OXPHOS) of brain mitochondria is exclusively and efficiently activated by extramitochondrial Ca2+ in physiological concentration ranges (S0.5= 360 nM Ca2+). This regulation was not affected by RR, an inhibitor of the mitochondrial Ca2+ uniporter. Active respiration is regulated by glutamate supply to mitochondria via aralar, a mitochondrial glutamate/aspartate carrier with regulatory Ca2+-binding sites in the mitochondrial intermembrane space providing full access to cytosolic Ca2+. At micromolar concentrations, Ca2+ can also enter the intramitochondrial matrix and activate specific dehydrogenases. However, the latter mechanism is less efficient than extramitochondrial Ca2+ regulation of respiration/OXPHOS via aralar. These results imply a new mode of glutamate-dependent OXPHOS regulation as a demand-driven regulation of mitochondrial function. This regulation involves the mitochondrial glutamate/aspartate carrier aralar which controls mitochondrial substrate supply according to the level of extramitochondrial Ca2+. β€’ Keywords: Glutamate/aspartate carrier aralar, Extramitochondrial Ca (2+), Brain mitochondria

β€’ O2k-Network Lab: EE_Tartu Paju K, DE Magdeburg Gellerich FN


Labels: MiParea: Respiration 


Tissue;cell: Nervous system 

Enzyme: Inner mt-membrane transporter  Regulation: Calcium, Inhibitor  Coupling state: OXPHOS 

HRR: Oxygraph-2k 

Ca-list