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Difference between revisions of "Gellerich 2009 PLoS One"

From Bioblast
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{{Labeling
{{Labeling
|instruments=Oxygraph-2k
|instruments=Oxygraph-2k
|tissues=Neurons; Brain
|tissues=Nervous system
|couplingstates=OXPHOS
|couplingstates=OXPHOS
|enzymes=Inner mtMembrane Transporter
|enzymes=Inner mtMembrane Transporter

Revision as of 08:47, 27 February 2013

Publications in the MiPMap
Gellerich FN, Gizatullina Z, Arandarcikaite O, Jerzembek D, Vielhaber S, Seppet E, Striggow F (2009) Extramitochondrial Ca2+ in the nanomolar range regulates glutamate-dependent oxidative phosphorylation on demand. PloS One 4: e8181.

Β» PMID: 20011041 Open Access

Gellerich FN, Gizatullina Z, Arandarcikaite O, Jerzembek D, Vielhaber S, Seppet E, Striggow F (2009) PLoS One

Abstract: We present unexpected and novel results revealing that glutamate-dependent oxidative phosphorylation (OXPHOS) of brain mitochondria is exclusively and efficiently activated by extramitochondrial Ca2+ in physiological concentration ranges (S0.5= 360 nM Ca2+). This regulation was not affected by RR, an inhibitor of the mitochondrial Ca2+ uniporter. Active respiration is regulated by glutamate supply to mitochondria via aralar, a mitochondrial glutamate/aspartate carrier with regulatory Ca2+-binding sites in the mitochondrial intermembrane space providing full access to cytosolic Ca2+. At micromolar concentrations, Ca2+ can also enter the intramitochondrial matrix and activate specific dehydrogenases. However, the latter mechanism is less efficient than extramitochondrial Ca2+ regulation of respiration/OXPHOS via aralar. These results imply a new mode of glutamate-dependent OXPHOS regulation as a demand-driven regulation of mitochondrial function. This regulation involves the mitochondrial glutamate/aspartate carrier aralar which controls mitochondrial substrate supply according to the level of extramitochondrial Ca2+. β€’ Keywords: Glutamate/aspartate carrier aralar, Extramitochondrial Ca (2+), Brain mitochondria

β€’ O2k-Network Lab: EE_Tartu_Seppet EK, DE_Magdeburg_Gellerich FN, DE Magdeburg Klinik Neurologie


Labels:


Tissue;cell: Nervous system 

Enzyme: Inner mtMembrane Transporter"Inner mtMembrane Transporter" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property. 

Coupling state: OXPHOS 

HRR: Oxygraph-2k