Difference between revisions of "Glutamate"
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|description='''Glutamate''' as the sole substrate is transported by the electroneutral glutamate-/OH- exchanger, and is oxidized in the mitochondrial matrix via [http://en.wikipedia.org/wiki/Glutamate_dehydrogenase glutamate dehydrogenase] to α-Ketoglutarate. Ammonia (the byproduct of the reaction) can pass freely through the mitochondrial membrane. See also [[Glutamate-aspartate carrier]]. | |description='''Glutamate''' as the sole substrate is transported by the electroneutral glutamate-/OH- exchanger, and is oxidized in the mitochondrial matrix via [http://en.wikipedia.org/wiki/Glutamate_dehydrogenase glutamate dehydrogenase] to α-Ketoglutarate. Ammonia (the byproduct of the reaction) can pass freely through the mitochondrial membrane. See also [[Glutamate-aspartate carrier]]. | ||
|info=[[MiPNet11.04]]; [[MiPNet09.12]] | |info=[[MiPNet11.04]]; [[MiPNet09.12]] | ||
|type=Respiration | |||
}} | |||
{{MitoPedia topics | |||
|mitopedia topic=Substrate and metabolite | |||
|type=Respiration | |type=Respiration | ||
}} | }} | ||
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|type=Respiration | |type=Respiration | ||
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
Revision as of 14:28, 9 March 2011
- high-resolution terminology - matching measurements at high-resolution
Glutamate
Description
Glutamate as the sole substrate is transported by the electroneutral glutamate-/OH- exchanger, and is oxidized in the mitochondrial matrix via glutamate dehydrogenase to α-Ketoglutarate. Ammonia (the byproduct of the reaction) can pass freely through the mitochondrial membrane. See also Glutamate-aspartate carrier.
Abbreviation: G
Reference: MiPNet11.04; MiPNet09.12
MitoPedia topics:
Substrate and metabolite
Labels:
Enzyme: Inner mtMembrane Transporter"Inner mtMembrane Transporter" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property. Regulation: Substrate; Glucose; TCA Cycle"Substrate; Glucose; TCA Cycle" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Chemicals; Media"Chemicals; Media" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
Application in HRR
G: Glutamate (L-Glutamic acid, sodium salt, C5H8NO4Na; contains 1 mol/mol H2O); Sigma G 1626, 100 g, store at R.T.: FW = 187.1 or 169.1 anhydrous)
Preparation of 2 M stock solution (dissolved in H2O):
- 1) weigh 1.871 g of L-Glutamic acid and transfer to 5 mL volumetric glass flask
- 2) add 3 ml H2O (pH = 6)
- 3) neutralize with 5 N KOH (approx. 50 µl)
- 4) check pH and adjust to 7 if necessary
- 5) adjust final volume to 5 ml and divide into 0.5 ml portions.
- 6) store frozen at -20 °C
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 10 µl using a 25 µl syringe
- Final conc. in 2 ml O2k-chamber: 10 mM
