Glutamate

high-resolution terminology - matching measurements at high-resolution

Glutamate

Description

Glutamate as the sole substrate is transported by the electroneutral glutamate-/OH- exchanger, and is oxidized in the mitochondrial matrix via glutamate dehydrogenase to α-Ketoglutarate. Ammonia (the byproduct of the reaction) can pass freely through the mitochondrial membrane. See also Glutamate-aspartate carrier.

Abbreviation: G

Reference: MiPNet11.04; MiPNet09.12

MitoPedia topics: Substrate and metabolite 

Application in HRR

G: Glutamate (L-Glutamic acid, sodium salt, C₅H₈NO₄Na; contains 1 mol/mol H₂O); Sigma G 1626, 100 g, store at R.T.: FW = 187.1 or 169.1 anhydrous)

Preparation of 2 M stock solution (dissolved in H₂O):

1) weigh 1.871 g of L-Glutamic acid and transfer to 5 mL volumetric glass flask

2) add 3 ml H₂O (pH = 6)

3) neutralize with 5 N KOH (approx. 50 µl)

4) check pH and adjust to 7 if necessary

5) adjust final volume to 5 ml and divide into 0.5 ml portions.

6) store frozen at -20 °C

Oxygraph-2k Manual Titrations: MiPNet09.12

• Titration volume: 10 µl using a 25 µl syringe
• Final conc. in 2 ml O2k-chamber: 10 mM