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Glutamate

From Bioblast


high-resolution terminology - matching measurements at high-resolution


Glutamate

Description

Glutamate as the sole substrate is transported by the electroneutral glutamate-/OH- exchanger, and is oxidized in the mitochondrial matrix via glutamate dehydrogenase to ฮฑ-Ketoglutarate. Ammonia (the byproduct of the reaction) can pass freely through the mitochondrial membrane. See also Glutamate-aspartate carrier.

Abbreviation: G

Reference: MiPNet11.04; MiPNet09.12


MitoPedia topics: Substrate and metabolite 

Application in HRR

G: Glutamate (L-Glutamic acid, sodium salt, C5H8NO4Na; contains 1 mol H2O /mol); Sigma G 1626, 100 g, store at R.T.; FW = 187.1 or 169.1 anhydrous)

Preparation of 2 M stock solution (dissolved in H2O):

1) weigh 1.871 g (or 1.691 g of anhydrous) L-Glutamic acid and transfer to 5 mL volumetric glass flask
2) add 3 ml H2O (pH = 6)
3) neutralize with 5 N KOH
4) check pH and adjust to 7 if necessary
5) adjust final volume to 5 ml and divide into 0.5 ml portions.
6) store frozen at -20 ยฐC


Oxygraph-2k Manual Titrations: MiPNet09.12

  • Titration volume: 10 ยตl using a 25 ยตl syringe
  • Final conc. in 2 ml O2k-chamber: 10 mM