Hoffmann 2018 Sci Rep

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Hoffmann C, Hรถckele S, Kappler L, Hrabฤ• de Angelis M, Hรคring HU, Weigert C (2018) The effect of differentiation and TGFฮฒ on mitochondrial respiration and mitochondrial enzyme abundance in cultured primary human skeletal muscle cells. Sci Rep 8:737.

ยป PMID: 29335583

Hoffmann C, Hoeckele S, Kappler L, Hrabe de Angelis M, Haering HU, Weigert C (2018) Sci Rep

Abstract: Measuring mitochondrial respiration in cultured cells is a valuable tool to investigate the influence of physiological and disease-related factors on cellular metabolism; however, the details of the experimental workflow greatly influence the informative value of the results. Working with primary cells and cell types capable of differentiation can be particularly challenging. We present a streamlined workflow optimised for investigation of primary human skeletal muscle cells. We applied the workflow to differentiated and undifferentiated cells and we investigated the effect of TGFฮฒ1 treatment. Differentiation of myoblasts to myotubes increased mitochondrial respiration and abundance of mitochondrial enzymes and mitochondrial marker proteins. Differentiation also induced qualitative changes in mitochondrial protein composition and respiration. TGFฮฒ1 reduced complex IV protein MTCO1 abundance in both myoblasts and myotubes. In myoblasts, spare electron transport system (ETS) capacity was reduced due to a reduction in maximal oxygen consumption. In TGFฮฒ1-treated myotubes, the reduction in spare ETS capacity is mainly a consequence of increased oxidative phosphorylation capacity and complex III protein UQCRC2. Taken together, our data shows that it is important to monitor muscle cell differentiation when mitochondrial function is studied. Our workflow is not only sensitive enough to detect physiological-sized differences, but also adequate to form mechanistic hypotheses.

โ€ข Bioblast editor: Kandolf G โ€ข O2k-Network Lab: DE Tuebingen Weigert C

Labels: MiParea: Respiration 

Organism: Human  Tissue;cell: Skeletal muscle  Preparation: Permeabilized cells 

Coupling state: OXPHOS, ET  Pathway: F, N, S, NS, ROX  HRR: Oxygraph-2k 

Labels, 2018-03 

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