Jacobs 2013 J Gerontol A Biol Sci Med Sci

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Jacobs R, Diaz V, Soldini L, Haider T, Thomassen M, Nordsborg NB, Gassmann M, Lundby C (2013) Fast-twitch glycolytic skeletal muscle is predisposed to age-induced impairments in mitochondrial function. J Gerontol A Biol Sci Med Sci 68:1010-22.

» PMID: 23371970

Jacobs R, Diaz V, Soldini L, Haider T, Thomassen M, Nordsborg NB, Gassmann M, Lundby C (2013) J Gerontol A Biol Sci Med Sci

Abstract: The etiology of mammalian senescence is suggested to involve the progressive impairment of mitochondrial function; however, direct observations of age-induced alterations in actual respiratory chain function are lacking. Accordingly, we assessed mitochondrial function via high-resolution respirometry and mitochondrial protein expression in soleus, quadricep, and lateral gastrocnemius skeletal muscles, which represent type 1 slow-twitch oxidative muscle (soleus) and type 2 fast-twitch glycolytic muscle (quadricep and gastrocnemius), respectively, in young (10-12 weeks) and mature (74-76 weeks) mice. Electron transport through mitochondrial complexes I and III increases with age in quadricep and gastrocnemius, which is not observed in soleus. Mitochondrial coupling efficiency during respiration through complex I also deteriorates with age in gastrocnemius and shows a tendency (p = .085) to worsen in quadricep. These data demonstrate actual alterations in electron transport function that occurs with age and are dependent on skeletal muscle type. Keywords: Senescence, Slow-twitch oxidative muscle, Type 2 fast-twitch glycolytic muscle, Function theory of aging.; Mitochondria; Respiratory chain

O2k-Network Lab: CH Zurich Lundby C, CH Zurich Gassmann M

Labels: MiParea: Respiration  Pathology: Aging;senescence 

Organism: Mouse  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue  Enzyme: Complex I, Complex III 

Coupling state: LEAK, OXPHOS, ET  Pathway: F, N, S, NS, ROX  HRR: Oxygraph-2k