Difference between revisions of "Jorgensen 2009 Am J Physiol Endocrinol Metab"

Latest revision as of 20:26, 12 November 2019

Jørgensen W, Gam C, Andersen JL, Schjerling P, Scheibye-Knudsen M, Mortensen OH, Grunnet N, Nielsen MO, Quistorff B (2009) Changed mitochondrial function by pre- and/or postpartum diet alterations in sheep. Am J Physiol Endocrinol Metab 297:E1349-57.

» PMID: 19826104 Open Access

Joergensen W, Gam C, Andersen JL, Schjerling P, Scheibye-Knudsen M, Mortensen OH, Grunnet N, Nielsen MO, Quistorff B (2009) Am J Physiol Endocrinol Metab

Abstract: In a sheep model, we investigated diet effects on skeletal muscle mitochondria to look for fetal programming. During pregnancy, ewes were fed normally (N) or were 50 % food restricted (L) during the last trimester, and lambs born to these ewes received a normal (N) or a high-fat diet (H) for the first 6 mo of life. We examined mitochondrial function in permeabilized muscle fibers from the lambs at 6 mo of age (adolescence) and after 24 mo of age (adulthood). The postpartum H diet for the lambs induced an ~30 % increase (P < 0.05) of mitochondrial V_O2max and an ~50 % increase (P < 0.05) of the respiratory coupling ratio (RCR) combined with lower levels of UCP3 and PGC-1αmRNA levels (P < 0.05). These effects proved to be reversible by a normal diet from 6 to 24 mo of age. However, at 24 mo, a long-term effect of the maternal gestational diet restriction (fetal programming) became evident as a lower V_O2max (~40 %, P < 0.05), a lower state 4 respiration (~40 %, P < 0.05), and lower RCR (~15 %, P < 0.05). Both PGC-1α and UCP3 mRNA levels were increased (P < 0.05). Two analyzed muscles were affected differently, and muscle rich in type I fibers was more susceptible to fetal programming. We conclude that fetal programming, seen as a reduced V_O2max in adulthood, results from gestational undernutrition. Postnatal high-fat diet results in a pronounced RCR and V_O2max increase in adolescence. However, these effects are reversible by diet correction and are not maintained in adulthood. • Keywords: Metabolic syndrome, High-fat diet, Nutrient restriction, Maternal diet, Respiratory coupling control ratio

• O2k-Network Lab: DK Copenhagen Quistorff B

Labels: MiParea: Respiration

Organism: Other mammals Tissue;cell: Skeletal muscle Preparation: Permeabilized tissue

Coupling state: OXPHOS

HRR: Oxygraph-2k

@@ Line 5: / Line 5: @@
 |year=2009
 |journal=Am J Physiol Endocrinol Metab
-|abstract=In a sheep model, we investigated diet effects on skeletal muscle mitochondria to look for fetal programming. During pregnancy, ewes were fed normally (N) or were 50% food restricted (L) during the last trimester, and lambs born to these ewes received a normal (N) or a high-fat diet (H) for the first 6 mo of life. We examined mitochondrial function in permeabilized muscle fibers from the lambs at 6 mo of age (adolescence) and after 24 mo of age (adulthood). The postpartum H diet for the lambs induced an ~30% increase (''P'' < 0.05) of mitochondrial V<sub>O2max</sub> and an ~50% increase (''P'' < 0.05) of the respiratory coupling ratio (RCR) combined with lower levels of UCP3 and PGC-1αmRNA levels (P < 0.05). These effects proved to be reversible by a normal diet from 6 to 24 mo of age. However, at 24 mo, a long-term effect of the maternal gestational diet restriction (fetal programming) became evident as a lower VO2max (~40%, ''P'' < 0.05), a lower state 4 respiration (~40%, ''P'' < 0.05), and lower RCR (~15%, ''P'' < 0.05). Both [[PGC-1α]] and UCP3 mRNA levels were increased (''P'' < 0.05). Two analyzed muscles were affected differently, and muscle rich in type I fibers was more susceptible to fetal programming. We conclude that fetal programming, seen as a reduced V<sub>O2max</sub>   in adulthood, results from gestational undernutrition. Postnatal high-fat diet results in a pronounced RCR and V<sub>O2max</sub> increase in adolescence. However, these effects are reversible by diet correction and are not maintained in adulthood.
+|abstract=In a sheep model, we investigated diet effects on skeletal muscle mitochondria to look for fetal programming. During pregnancy, ewes were fed normally (N) or were 50 % food restricted (L) during the last trimester, and lambs born to these ewes received a normal (N) or a high-fat diet (H) for the first 6 mo of life. We examined mitochondrial function in permeabilized muscle fibers from the lambs at 6 mo of age (adolescence) and after 24 mo of age (adulthood). The postpartum H diet for the lambs induced an ~30 % increase (''P'' < 0.05) of mitochondrial ''V''<sub>O2max</sub> and an ~50 % increase (''P'' < 0.05) of the respiratory coupling ratio (RCR) combined with lower levels of UCP3 and PGC-1αmRNA levels (''P'' < 0.05). These effects proved to be reversible by a normal diet from 6 to 24 mo of age. However, at 24 mo, a long-term effect of the maternal gestational diet restriction (fetal programming) became evident as a lower ''V''<sub>O2max</sub> (~40 %, ''P'' < 0.05), a lower [[state 4]] respiration (~40 %, ''P'' < 0.05), and lower [[RCR]] (~15 %, ''P'' < 0.05). Both [[PGC-1α]] and UCP3 mRNA levels were increased (''P'' < 0.05). Two analyzed muscles were affected differently, and muscle rich in type I fibers was more susceptible to fetal programming. We conclude that fetal programming, seen as a reduced ''V''<sub>O2max</sub> in adulthood, results from gestational undernutrition. Postnatal high-fat diet results in a pronounced RCR and ''V''<sub>O2max</sub> increase in adolescence. However, these effects are reversible by diet correction and are not maintained in adulthood.
 |keywords=Metabolic syndrome, High-fat diet, Nutrient restriction, Maternal diet, Respiratory coupling control ratio
 |mipnetlab=DK Copenhagen Quistorff B