Difference between revisions of "Least squares method"
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|description=This method makes use of all of the data points of the spectrum in order to quantify a measured spectrum with a reference spectrum of known concentration using a '''least squares method''' to match the measured spectrum with the reference spectrum. The technique results in improved accuracy compared with the use of only a few characteristic wavelengths. | |description=This method makes use of all of the data points of the spectrum in order to quantify a measured spectrum with a reference spectrum of known concentration using a '''least squares method''' to match the measured spectrum with the reference spectrum. The technique results in improved accuracy compared with the use of only a few characteristic wavelengths. | ||
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{{MitoPedia methods | {{MitoPedia methods | ||
|mitopedia method=Fluorometry, Spectrophotometry | |mitopedia method=Fluorometry, Spectrophotometry | ||
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Latest revision as of 14:31, 8 February 2016
- high-resolution terminology - matching measurements at high-resolution
Least squares method
Description
This method makes use of all of the data points of the spectrum in order to quantify a measured spectrum with a reference spectrum of known concentration using a least squares method to match the measured spectrum with the reference spectrum. The technique results in improved accuracy compared with the use of only a few characteristic wavelengths.
MitoPedia methods: Fluorometry, Spectrophotometry
