Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "Makrecka-Kuka 2018 MiP2018"

From Bioblast
(Created page with "{{Abstract |title=left|90px|Marina Makrecka-Kuka |info=MiP2018 |year=2018 |event=MiP2018 |abstract=Image:MITOEAGLE-logo.jpg|left|100px|link=http:...")
 
Line 1: Line 1:
{{Abstract
{{Abstract
|title=[[Image:MakreckaM.jpg|left|90px|Marina Makrecka-Kuka]]
|title=[[Image:MakreckaM.jpg|left|90px|Marina Makrecka-Kuka]] Fatty acid oxidation in brain: from aging to ischemia and sepsis.
|info=[[MiP2018]]
|info=[[MiP2018]]
|authors=Makrecka-Kuka M, Doerrier C, Korzh S, Zvejniece L, Gnaiger E, Dambrova M, Liepinsh E
|year=2018
|year=2018
|event=MiP2018
|event=MiP2018
|abstract=[[Image:MITOEAGLE-logo.jpg|left|100px|link=http://www.mitoglobal.org/index.php/MITOEAGLE|COST Action MitoEAGLE]]
|abstract=[[Image:MITOEAGLE-logo.jpg|left|100px|link=http://www.mitoglobal.org/index.php/MITOEAGLE|COST Action MitoEAGLE]] The brain tissues have a high energy demand that is almost exclusively satisfied by metabolizing glucose. Recent studies have demonstrated that also fatty acids are utilized by brain mitochondria. However, the role of fatty acid oxidation (FAO) in brain tissues is still unclear. The aim of the present study was to evaluate the fatty acid oxidation in brain under physiological and pathological conditions.
 
Mitochondrial respiration was measured using High-Resolution Fluoespirometry in brain tissue homogenates. The measurements were performed at OXPHOS state using palmitoylcarnitine (PC) or octanoylcarnitine (OC) as substrates. The FAO was evaluated in brain tissues of adult (8 months) and old (20 months) Wistar male rats, as well as in tissues after endothelin-1 (ET-1) induced stroke. In addition, measurements were done using high fat diet-induced insulin resistance and LPS-induced endotoxic shock experimental mice models.
 
The measurements in control mice tissues demonstrated that FA-dependent O<sub>2</sub> flux is higher using PC than OC. Moreover, PC, but not OC, inhibits pyruvate metabolism in brain. The PC-dependent O<sub>2</sub> flux was 2.3 times lower in old rat brain compared to adult rat group. In ET-1 induced experimental model of stroke we observed 31% and 66% decrease in PC-dependent O<sub>2</sub> flux 2h and 24h post- ET-1 injection in affected brain area compared to sham control.  The high-fat diet for 18 weeks resulted in a 56% reduction of PC-dependent O<sub>2</sub> flux in mice brain. Similarly, the PC-dependent O<sub>2</sub> flux was by 52% lower in LPS-treated mice compared to control group.
 
Taking into account FAO biochemical pathway and obtained results, the measurements using PC are more straight-forward to characterize FAO in mitochondria. All studied conditions induced an inhibition of FAO in brain, indicating that FAO and availability of FA metabolites could regulate physiological process as well as play a significant role in the development of neurological disorders. Targeting FAO and availability of FA metabolites provide a novel therapeutic approach for treatment of neuropathological states.
|editor=[[Plangger M]], [[Kandolf G]]
|editor=[[Plangger M]], [[Kandolf G]]
|mipnetlab=LV Riga Makrecka-Kuka M, AT Innsbruck Oroboros
}}
{{Labeling
|area=Exercise physiology;nutrition;life style
|diseases=Aging;senescence, Neurodegenerative, Sepsis
|injuries=Ischemia-reperfusion
|organism=Mouse, Rat
|tissues=Nervous system
|preparations=Homogenate
|topics=Fatty acid
|couplingstates=OXPHOS
|pathways=F
}}
}}
{{Labeling}}
== Affiliations ==
== Affiliations ==
:::Makrecka-Kuka Marina(1), Doerrier C(2), Korzh S(1), Zvejniece L(1), Gnaiger E(2,3), Dambrova M(1), Liepinsh E(1)
::::#Latvian Inst Organic Synthesis, Riga, Latvia. - makrecka@farm.osi.lv
::::#Oroboros Instruments, Innsbruck, Austria
::::#Medical Univ Innsbruck, Austria




== References ==
== Acknowledgments ==
This study was supported by “Post-doctoral Research Aid” programme project Nr.1.1.1.2/VIAA/1/16/246.

Revision as of 10:43, 24 August 2018

Marina Makrecka-Kuka
Fatty acid oxidation in brain: from aging to ischemia and sepsis.

Link: MiP2018

Makrecka-Kuka M, Doerrier C, Korzh S, Zvejniece L, Gnaiger E, Dambrova M, Liepinsh E (2018)

Event: MiP2018

COST Action MitoEAGLE

The brain tissues have a high energy demand that is almost exclusively satisfied by metabolizing glucose. Recent studies have demonstrated that also fatty acids are utilized by brain mitochondria. However, the role of fatty acid oxidation (FAO) in brain tissues is still unclear. The aim of the present study was to evaluate the fatty acid oxidation in brain under physiological and pathological conditions.

Mitochondrial respiration was measured using High-Resolution Fluoespirometry in brain tissue homogenates. The measurements were performed at OXPHOS state using palmitoylcarnitine (PC) or octanoylcarnitine (OC) as substrates. The FAO was evaluated in brain tissues of adult (8 months) and old (20 months) Wistar male rats, as well as in tissues after endothelin-1 (ET-1) induced stroke. In addition, measurements were done using high fat diet-induced insulin resistance and LPS-induced endotoxic shock experimental mice models.

The measurements in control mice tissues demonstrated that FA-dependent O2 flux is higher using PC than OC. Moreover, PC, but not OC, inhibits pyruvate metabolism in brain. The PC-dependent O2 flux was 2.3 times lower in old rat brain compared to adult rat group. In ET-1 induced experimental model of stroke we observed 31% and 66% decrease in PC-dependent O2 flux 2h and 24h post- ET-1 injection in affected brain area compared to sham control. The high-fat diet for 18 weeks resulted in a 56% reduction of PC-dependent O2 flux in mice brain. Similarly, the PC-dependent O2 flux was by 52% lower in LPS-treated mice compared to control group.

Taking into account FAO biochemical pathway and obtained results, the measurements using PC are more straight-forward to characterize FAO in mitochondria. All studied conditions induced an inhibition of FAO in brain, indicating that FAO and availability of FA metabolites could regulate physiological process as well as play a significant role in the development of neurological disorders. Targeting FAO and availability of FA metabolites provide a novel therapeutic approach for treatment of neuropathological states.


Bioblast editor: Plangger M, Kandolf G O2k-Network Lab: LV Riga Makrecka-Kuka M, AT Innsbruck Oroboros


Labels: MiParea: Exercise physiology;nutrition;life style  Pathology: Aging;senescence, Neurodegenerative, Sepsis  Stress:Ischemia-reperfusion  Organism: Mouse, Rat  Tissue;cell: Nervous system  Preparation: Homogenate 

Regulation: Fatty acid  Coupling state: OXPHOS  Pathway:



Affiliations

Makrecka-Kuka Marina(1), Doerrier C(2), Korzh S(1), Zvejniece L(1), Gnaiger E(2,3), Dambrova M(1), Liepinsh E(1)
  1. Latvian Inst Organic Synthesis, Riga, Latvia. - makrecka@farm.osi.lv
  2. Oroboros Instruments, Innsbruck, Austria
  3. Medical Univ Innsbruck, Austria


Acknowledgments

This study was supported by “Post-doctoral Research Aid” programme project Nr.1.1.1.2/VIAA/1/16/246.