View source for Melo Hanchuk 2015 Cell Signal

{{Publication
|title=Melo Hanchuk TD, Papa PF, La Guardia PG, Vercesi AE, Kobarg J (2015) Nek5 interacts with mitochondrial proteins and interferes negatively in mitochondrial mediated cell death and respiration. Cell Signal 27:1168-77.
|info=[http://www.ncbi.nlm.nih.gov/pubmed/25725288 PMID:25725288]
|authors=Melo Hanchuk TD, Papa PF, La Guardia PG, Vercesi AE, Kobarg J
|year=2015
|journal=Cell Signal
|abstract=Mitochondria are involved in energy supply, signaling, cell death and cellular differentiation and have been implicated in several human diseases. Neks (NIMA-related kinases) represent a family of mammal protein kinases that play essential roles in cell-cycle progression, but other functions have recently been related. A yeast two-hybrid (Y2H) screen was performed to identify and characterize Nek5 interaction partners and the mitochondrial proteins Cox11, MTX-2 and BCLAF1 were retrieved. Apoptosis assay showed protective effects of stable hNek5 expression from Hek293-T's cell death after thapsigargin treatment (2 μM). Nek5 silenced cells as well as cells expressing a "kinase dead" version of Nek5, displayed an increase in ROS formation after 4h of thapsigargin treatment. Mitochondrial respiratory chain activity was found decreased upon stable hNek5expression. Cells silenced for hNek5 on the other hand presented 1.7 fold increased basal rates of respiration, especially at the electrons transfer steps from TMPD to cytochrome c and at the complex II. In conclusion, our data suggest for the first time mitochondrial localization and functions for Nek5 and its participation in cell death and cell respiration regulation. Stable expression of hNek5 in Hek293T cells resulted in enhanced cell viability, decreased cell death and drug resistance, while depletion of hNek5by shRNA overcame cancer cell drug resistance and induced apoptosis ''in vitro''. Stable expression of hNek5 also inhibits thapsigargin promoted apoptosis and the respiratory chain complex IV in HEK293T cells.
|keywords=Mitochondria, Reactive oxygen species, Respiratory chain, hNek5
|mipnetlab=BR Campinas Vercesi AE
}}
{{Labeling
|area=Respiration
|organism=Human
|tissues=HEK
|preparations=Permeabilized cells
|couplingstates=LEAK, OXPHOS
|pathways=N, S, CIV, ROX
|instruments=Oxygraph-2k
}}