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Difference between revisions of "MiPNet06.03 POS-calibration-SOP"

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{{OROBOROS header page name}}
{{Template:OROBOROS support page name}}
{{Publication
{{Publication
|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration.
|title=[[Image:O2k-Manual.jpg|right|70px|link=O2k-Manual|O2k-Manual]] O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration.
|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/7/77/MiPNet06.03_POS-Calibration-SOP.pdf |Bioblast pdf]] |authors=Oroboros
|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/7/77/MiPNet06.03_POS-Calibration-SOP.pdf |Bioblast pdf]] Β» [http://www.bioblast.at/index.php/File:MiPNet06.03_POS-Calibration-SOP.pdf Versions]
|year=2018-01-07
|authors=Oroboros
|year=2020-02-16
|journal=Mitochondr Physiol Network
|journal=Mitochondr Physiol Network
|abstract='''Gnaiger E (2018) O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration. Mitochondr Physiol Network 06.03(17):1-20.''' Β» [http://www.bioblast.at/index.php/File:MiPNet06.03_POS-Calibration-SOP.pdf Versions]
|abstract=Gnaiger E (2020) O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration. Mitochondr Physiol Network 06.03(18):1-21.
Β 
{{MiPNet pdf page linking to MitoPedia}}
'''O2k-Protocols SOP''': High-resolution respirometry (HRR; Oroboros O2k) critically depends on maintenance POS-service and accurate calibration of the polarographic oxygen sensors ([[OroboPOS]], POS). Standard operating procedures (O2k-SOP) are described as part of the [[MitoFit Quality Control System]]. (1) Clean and ready-to-use O2k-Chambers are a basic requirement. (2) Quality control for evaluation of proper POS function (SOP: O2 sensor test including the [[stirrer test]]). (3) Accurate POS calibration ([[air calibration]] and [[zero calibration]]). This is part 1 of O2k Quality Control.
Β 
:Β» O2k-Protocols SOP: O2k Quality Control 2 Β»[[MiPNet14.06 Instrumental O2 background]]
:Β» O2k-Manual: [[MiPNet19.18D_O2k-calibration]]
:Β» Product: [[OroboPOS]], [[Oroboros O2k]], [[Oroboros O2k-Catalogue |O2k-Catalogue]]
:Β» Template: [[File:O2 calibration.xlsx|O2 calibration.xlsx]]
|mipnetlab=AT_Innsbruck_Oroboros
|mipnetlab=AT_Innsbruck_Oroboros
}}
}}
{{Labeling
{{Labeling
|area=Instruments;methods
|area=Respiration, Instruments;methods
|instruments=Oxygraph-2k, O2k-Protocol
|instruments=Oxygraph-2k, O2k-Manual
|additional=O2k-SOP
|additional=DatLab, O2k-SOP, DL7, Oxygen solubility, MitoFit 2021 PT, MitoFit 2021 Dark respiration, MitoFit 2021 Photosynthesis
}}
}}
<br />
[[File:Expand.png|right|45px |Click to expand or collaps]]
<div class="toccolours mw-collapsible mw-collapsed">
:::[[File:Questions.jpg|left|24px |Explanation of terms]] '''Β» Keywords'''
<div class="mw-collapsible-content">
::: '''Instrument'''
::::Β» [[O2k-Core]]
::::Β» [[MiPNet19.18D O2k-calibration |O2k-Manual: O2k-calibration]]
::::Β» [[MiPNet19.18B POS-service |O2k-Manual: POS-service]]
::::Β» [[MiPNet19.03 O2k-cleaning and ISS |O2k-Protocols SOP: O2k-cleaning]]
::::Β» [[OroboPOS]]
::::Β» [[Polarographic oxygen sensor]]
::::Β» [[Stirrer test]]
::: '''Concept'''
::::Β» [[Air calibration]]
::::Β» [[Concentration]]
::::Β» [[High-resolution respirometry]]
::::Β» [[MiPNet06.03 POS-calibration-SOP |O2k-Protocols SOP: POS-calibration]]
::::Β» [[MitoFit Quality Control System]]
::::Β» [[O2k-SOP |O2k-Protocols SOP]]
::::Β» [[Oxygen |Oxygen, dioxygen, O<sub>2</sub>]]
::::Β» [[Oxygen sensor test]]
::::Β» [[Zero calibration]]
</div>
</div>
<br />
__TOC__
{{Technical support integrated}}
<br>
== Standard operating procedure ==
::::The O2 sensor test does not only serve to evaluate the function of the OroboPOS, but many other components of the O2k have to function according to specifications for a high-resolution oxygen signal to be obtained:
::::# [[USB-Cable 2.0\Type A-B]] not properly connected to the O2k and PC or Laptop.
::::# [[O2k-Chamber]] not properly positioned, such that O2 sensors are not connected to the medium.
::::# [[OroboPOS-Membranes]] defective or not properly applied.
::::# [[OroboPOS-Connector]] contaminated gold contacts; plugs not properly connected to the sockets of the [[O2k-Main Unit]].
::::# OroboPOS contacts not cleaned. In rare cases, [[Pen-Contact Oil]] might be used.
::::# [[OroboPOS-Electrolyte Powder]] contaminated, inappropriate water used for dissolving the powder.
::::# [[O2-Zero Powder]] not properly handled; confused with [[OroboPOS-Polishing Powder]].
::::# [[OroboPOS]] not properly serviced; not properly mounted to the [[OroboPOS-Connector]]; or defective POS head.
::::# Room temperature not sufficiently stable.
::::# [[Stirrer-Bar\white PVDF\15x6 mm]] not added to the chamber, or stuck and not rotating.
::::# [[O2k-Barometric Pressure Transducer]] not properly calibrated.
::::# [[O2k-Peltier Temperature Control]] defective electronics.
::::# [[O2k-Electromagnetic Stirrer Twin-Control]] defective electronics.
::::# [[O2k-Main Basic]] not properly connected; other defective hardware.
== Trouble shooting ==
:::'''If the signal remains off scale''' (9.99 V) or very low (< 1 V) at air saturation (25 to 37 Β°C; lower signals at lower temperatures): Many components may be responsible, and an electronic defect of the [[O2k-Main Unit]] must be carefully excluded.
::::# Check settings for [[Gain]] (use Gain 1) and [[Polarization voltage]] (use 800 mV). If these settings were incorrect, the off-scale problem may be solved simply by using the standard settings.
::::# Empty the chamber with the O2k running and connected to DatLab. Switch the stirrer off. Remove the [[OroboPOS-Connector]] with the attached [[OroboPOS]]. Leave the sensor attached to the POS connector, and the POS connector plugged into the O2k-Main Unit. Record the signal for some minutes. The raw signal should normalize to a value >1 V and <3 V (Gain 1). If so, the O2k-Chamber assembly was problematic (application problem), and re-assembly will solve the problem. Β» [https://www.youtube.com/watch?v=1JffCpapX6A O2k-Videosupport: Insert O2k-Chamber].
::::# Remove the sensor head from the sensor connector, which remains plugged into the O2k-Main Unit. If the raw signal is not stable at 0 V, a defect of the [[OroboPOS-Connector]] is indicated.
::::# Remove the plug of the [[OroboPOS-Connector]] from the [[O2k-Main Unit]]. A signal of 0.4 V, which remains stable when changing the gain, is a strong indication of an electronic problem in the O2k-Main Unit.
::::## For O2k-Series D and higher: The signal should be 0 V, otherwise an electronic problem of the O2k-Main Unit is indicated.
::::## For O2k-Series A to C: The signal should be off-scale (+/- 9.99 V), otherwise an electronic problem of the O2k-Main Unit is indicated.
:::'''If specifications given in the [[MiPNet06.03 POS-calibration-SOP |POS-SOP]] are not obtained''': switch components for locating the problem.
::::# Switch the [[OroboPOS]] between O2k-Chambers A and B. This is a good opportunity to clean the gold contacts and apply [[Contact oil]] to the gold pin and thread connecting the [[POS connector]]s and sensors. It is not necessary to remove the seal tip and the mounted membrane from the OroboPOS. When disconnecting a sensor from the POS connector, prevent damage by electrostatic discharge by following the guidelines: [[MiPNet14.01 ESD-damage]].
::::# Repeat the O2 sensor test. If the problem moved together with the sensor from one side to the other, the problem is located in the [[OroboPOS]] sensor.
::::# If the problem remained on the same side, switch the [[POS connector]] together with the attached sensors between right and left side (A and B).
::::# Repeat the O2 sensor test. If the problem moved together with the POS connector, it is located at the [[OroboPOS-Connector]].
== Oxygen solubility factor ==
[[Image:MiPNet06.03_Table.jpg|centre|frame|<sup>c</sup>O2* is the oxygen concentration at thermodynamic equilibrium with air under standard conditions of 100 kPa barometric pressure. ''F''<sub>M</sub> has been determined for MiR05 at 30 Β°C and 37 Β°C, and is assumed to be identical at 25 Β°C.]]
== Instrumental O2 DLP ==
Instrumental [[DL-Protocols]] (DLP) are used for calibrations and instrumental quality control, typically without experimental sample in the incubation medium.
{| class="wikitable" border="1"
|-
! DL-Protocol
! Description
! DLP File
|-
| O2 calibration air
| O2 sensor test and calibration at air saturation.
| [[Media:O2-calibration_air.DLP|O2 calibration air.DLP]]
|-
| O2 calibration air and zero
| O2 sensor test and calibration at air saturation and zero oxygen.Β 
| [[Media:O2 calibration air and zero.DLP|O2 calibration air and zero.DLP]]
|}
A full list of DL-Protocols (Instrumental O2 DL-Protocols and SUIT O2 DL-Protocols) is displayedΒ  in [[DL-Protocols]].


== References ==
::::* Forstner H, Gnaiger E (1983) Calculation of equilibrium oxygen concentration. In: Polarographic Oxygen Sensors. Aquatic and Physiological Applications. Gnaiger E, Forstner H (eds), Springer, Berlin, Heidelberg, New York:321-33. - [[Forstner_1983_POS |Β»Bioblast link]]
::::* [[Gnaiger 2008 POS]]
::::* [[MiPNet19.18D O2k-calibration]]


::: '''This article is cited in'''
::::* Rodriguez JV, Pizarro MD, Scandizzi AL, Guibert EE, Almada LL, Mamprin ME (2008) Construction and performance of a minibioreactor suitable as experimental bioartificial liver. Artif Organs 32:323-8. [http://www.ncbi.nlm.nih.gov/pubmed/18370948 Β»PubMed 18370948]


{{Template:Keywords: Oxygen signal}}


::::Β» [[Oxygen calibration - DatLab]]


[[File:O2k-QCS.jpg|350px|O2k-QCS]] Next step - O2k Quality Control 2: [[MiPNet14.06 Instrumental O2 background]]




[[Category:Technical service]]
== Cited by ==
{{Template:Cited by Huete-Ortega M 2021 MitoFit Dark respiration}}
{{Template:Cited by Huete-Ortega M 2021 MitoFit Photosynthesis protocols}}

Revision as of 15:38, 25 February 2021


                  


O2k-Open Support

MiPNet06.03 POS-calibration-SOP


Publications in the MiPMap
O2k-Manual
O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration.

Β» Bioblast pdf  Β» Versions

Oroboros (2020-02-16) Mitochondr Physiol Network

Abstract: Gnaiger E (2020) O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration. Mitochondr Physiol Network 06.03(18):1-21.

O2k-technical support and open innovation
Open the pdf document above.
Β» Current O2k-series: NextGen-O2k Series XB and O2k Series J
Β» Current software versions DatLab 8.0: MitoPedia: DatLab


β€’ O2k-Network Lab: AT_Innsbruck_Oroboros


Labels: MiParea: Respiration, Instruments;methods 





HRR: Oxygraph-2k, O2k-Manual 

DatLab, O2k-SOP, DL7, Oxygen solubility, MitoFit 2021 PT, MitoFit 2021 Dark respiration, MitoFit 2021 Photosynthesis 




Questions.jpg


Click to expand or collaps


Β» Oxygen calibration - DatLab


Cited by

  • Huete-Ortega et al (2021) Substrate-uncoupler-inhibitor-titration protocols for dark respiration in Chlamydomonas reinhardtii. MitoFit Preprints 2021 (in prep).
  • Huete-Ortega et al (2021) Substrate-uncoupler-inhibitor-titration protocols for photosynthesis in Chlamydomonas reinhardtii. MitoFit Preprints 2021 (in prep).