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Difference between revisions of "MiPNet09.12 O2k-Titrations"

From Bioblast
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|abstract='''Oroboros (2018) O2k manual titrations: SUIT protocols with mitochondrial preparations. Mitochondr Physiol Network 09.12(17): 1-2.'''   
|abstract='''Oroboros (2018) O2k manual titrations: SUIT protocols with mitochondrial preparations. Mitochondr Physiol Network 09.12(17): 1-2.'''   


A list of substrates, uncouplers, and inhibitors used in [[SUIT]] protocols in [[High-Resolution FluoRespirometry]], with information on abbreviations, stock concentrations, final experimental concentrations, volumes titrated into the 2 ml O2k-chamber, and syringes used for manual titrations.
A list of substrates, uncouplers, and inhibitors used in [[SUIT]] protocols in [[high-resolution respirometry]], with information on abbreviations, stock concentrations, final experimental concentrations, volumes titrated into the 2 mL O2k-chamber, and syringes used for manual titrations.
:» [[O2k-Protocols]]
:» [[O2k-Protocols]]
:» Product: [[Oroboros O2k]], [[Oroboros O2k-Catalogue |O2k-Catalogue]]
:» Product: [[Oroboros O2k]], [[Oroboros O2k-Catalogue |O2k-Catalogue]]

Revision as of 10:04, 17 March 2019

Publications in the MiPMap
O2k-Protocols
O2k manual titrations: SUIT protocols with mitochondrial preparations.

» Bioblast pdf »Versions

Oroboros (2018-10-01) Mitochondr Physiol Network

Abstract: Oroboros (2018) O2k manual titrations: SUIT protocols with mitochondrial preparations. Mitochondr Physiol Network 09.12(17): 1-2.

A list of substrates, uncouplers, and inhibitors used in SUIT protocols in high-resolution respirometry, with information on abbreviations, stock concentrations, final experimental concentrations, volumes titrated into the 2 mL O2k-chamber, and syringes used for manual titrations.

» O2k-Protocols
» Product: Oroboros O2k, O2k-Catalogue


O2k-Network Lab: AT_Innsbruck_Oroboros


Labels: MiParea: Instruments;methods 




Regulation: ADP, Inhibitor, Substrate, Uncoupler, Amino acid 


HRR: O2k-Protocol 

O2k-chemicals and media 


Note: Titration of chemicals in respirometry

Overshoots or short interruptions of the flux after the injection of chemicals do not have a meaning in respirometric measurements. A disturbance of the signal is only observed due to the injected chemical and does not represent mitochondrial respiration. With the addition of a chemical solved in ethanol a little bit of oxygen is always introduced, since ethanol has higher oxygen solubility than water.


Further information

» MitoPedia: SUIT - Substrate-uncoupler-inhibitor-titration (SUIT) protocols for respirometry
» MiPNet03.02 - Chemicals and media for respirometry
» MiPNet19.14 - SOP for manual O2k-titrations with Hamilton microsyringes.