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MiPNet11.05 imt-pti-pce

From Bioblast
Publications in the MiPMap
Gnaiger E (2005) Isolated mitochondria or permeabilized tissues and cells. Mitochondr Physiol Network 11.05.

ยป Open Access.MiPNet11.05.pdf, Versions

O2k-Protocols contents

OROBOROS (2005) Mitochondr Physiol Network

Abstract: O2k-Protocol for Isolated mitochondria or permeabilized tissues and cells

Summary: Whereas isolated mitochondria remain one of the gold-standards in studies of bioenergetics and mitochondrial physiology, permeabilized tissues and cells have become an alternative with several advantages. But some disadvantages have to be considered, too, for optimum experimental design and critical evaluation of results.

>> O2k-Protocols:Overall contents
>> Product: OROBOROS Oxygraph-2k, O2k-Catalogue


โ€ข O2k-Network Lab: AT_Innsbruck_OROBOROS


Labels: MiParea: mt-Biogenesis;mt-density 



Preparation: Permeabilized cells, Permeabilized tissue, Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property. 



HRR: Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property. 


Further information

We recommend the new METTLER TOLEDO microbalances XS105DU or XS205DU for determination of tissue wet weight (1 to 3 mg wet weight per chamber). We offer these microbalances as part of our systems-approach to high-resolution respirometry.


  • Version 5: 2011-12-03
  • Version 1: 2005